Association of the cocaine and amphetamine-regulated transcript gene with type 2 diabetes mellitus

Mao Fu, Hua Cheng, Lihong Chen, Bin Wu, Mengyin Cai, Ding Xie, Zuzhi Fu

Research output: Contribution to journalArticle

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Abstract

OBJECTIVE: To investigate whether genetic variation in cocaine and amphetamine-regulated transcript (CART) gene might contribute to the genesis of type 2 diabetes. METHODS: Screening for mutations in the entire coding region for the CART gene were performed with polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) in 180 normoglycemic control subjects and 221 patients with type 2 diabetes. RESULTS: (1) Adenine deletion was identified at position 1,457 nucleotide located at untranslation area of exon 3. In normal weight control, the frequencies of CART-A + and CART-A-alleles were 83.6% and 16.4% respectively. The frequencies of CART-A + A +, A + A-, A-A- genotype were 68.9%, 29.4% and 1.7% respectively. (2) In diabetic patients, the frequencies of CART-A + and A-alleles were 84.6% and 15.4% respectively; the frequencies of CART-A + A +, A + A-, A-A- genotype were 71.9%, 25.3% and 2.7% respectively. The frequency of A deletion of the CART gene in diabetic patients did not differ significantly from normoglycemic control subjects. (3) Diabetic patients with the A deletion had increased total cholesterol, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol. CONCLUSION: Polymorphism was found in the 3'-untranslated region (Delta A1457) of CART in Chinese. A deletion in CART is not associated with type 2 diabetes, but may contribute to dyslipidemia.

Original languageEnglish (US)
Pages (from-to)805-808
Number of pages4
JournalZhonghua nei ke za zhi [Chinese journal of internal medicine]
Volume41
Issue number12
StatePublished - Jan 1 2002
Externally publishedYes

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Amphetamine
Cocaine
Type 2 Diabetes Mellitus
Genes
Alleles
Genotype
3' Untranslated Regions
Adenine
Dyslipidemias
LDL Cholesterol
HDL Cholesterol
Exons
Nucleotides
Cholesterol
Weights and Measures
Polymerase Chain Reaction
Mutation

ASJC Scopus subject areas

  • Medicine(all)

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Association of the cocaine and amphetamine-regulated transcript gene with type 2 diabetes mellitus. / Fu, Mao; Cheng, Hua; Chen, Lihong; Wu, Bin; Cai, Mengyin; Xie, Ding; Fu, Zuzhi.

In: Zhonghua nei ke za zhi [Chinese journal of internal medicine], Vol. 41, No. 12, 01.01.2002, p. 805-808.

Research output: Contribution to journalArticle

Fu, Mao ; Cheng, Hua ; Chen, Lihong ; Wu, Bin ; Cai, Mengyin ; Xie, Ding ; Fu, Zuzhi. / Association of the cocaine and amphetamine-regulated transcript gene with type 2 diabetes mellitus. In: Zhonghua nei ke za zhi [Chinese journal of internal medicine]. 2002 ; Vol. 41, No. 12. pp. 805-808.
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abstract = "OBJECTIVE: To investigate whether genetic variation in cocaine and amphetamine-regulated transcript (CART) gene might contribute to the genesis of type 2 diabetes. METHODS: Screening for mutations in the entire coding region for the CART gene were performed with polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) in 180 normoglycemic control subjects and 221 patients with type 2 diabetes. RESULTS: (1) Adenine deletion was identified at position 1,457 nucleotide located at untranslation area of exon 3. In normal weight control, the frequencies of CART-A + and CART-A-alleles were 83.6{\%} and 16.4{\%} respectively. The frequencies of CART-A + A +, A + A-, A-A- genotype were 68.9{\%}, 29.4{\%} and 1.7{\%} respectively. (2) In diabetic patients, the frequencies of CART-A + and A-alleles were 84.6{\%} and 15.4{\%} respectively; the frequencies of CART-A + A +, A + A-, A-A- genotype were 71.9{\%}, 25.3{\%} and 2.7{\%} respectively. The frequency of A deletion of the CART gene in diabetic patients did not differ significantly from normoglycemic control subjects. (3) Diabetic patients with the A deletion had increased total cholesterol, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol. CONCLUSION: Polymorphism was found in the 3'-untranslated region (Delta A1457) of CART in Chinese. A deletion in CART is not associated with type 2 diabetes, but may contribute to dyslipidemia.",
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AU - Xie, Ding

AU - Fu, Zuzhi

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N2 - OBJECTIVE: To investigate whether genetic variation in cocaine and amphetamine-regulated transcript (CART) gene might contribute to the genesis of type 2 diabetes. METHODS: Screening for mutations in the entire coding region for the CART gene were performed with polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) in 180 normoglycemic control subjects and 221 patients with type 2 diabetes. RESULTS: (1) Adenine deletion was identified at position 1,457 nucleotide located at untranslation area of exon 3. In normal weight control, the frequencies of CART-A + and CART-A-alleles were 83.6% and 16.4% respectively. The frequencies of CART-A + A +, A + A-, A-A- genotype were 68.9%, 29.4% and 1.7% respectively. (2) In diabetic patients, the frequencies of CART-A + and A-alleles were 84.6% and 15.4% respectively; the frequencies of CART-A + A +, A + A-, A-A- genotype were 71.9%, 25.3% and 2.7% respectively. The frequency of A deletion of the CART gene in diabetic patients did not differ significantly from normoglycemic control subjects. (3) Diabetic patients with the A deletion had increased total cholesterol, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol. CONCLUSION: Polymorphism was found in the 3'-untranslated region (Delta A1457) of CART in Chinese. A deletion in CART is not associated with type 2 diabetes, but may contribute to dyslipidemia.

AB - OBJECTIVE: To investigate whether genetic variation in cocaine and amphetamine-regulated transcript (CART) gene might contribute to the genesis of type 2 diabetes. METHODS: Screening for mutations in the entire coding region for the CART gene were performed with polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) in 180 normoglycemic control subjects and 221 patients with type 2 diabetes. RESULTS: (1) Adenine deletion was identified at position 1,457 nucleotide located at untranslation area of exon 3. In normal weight control, the frequencies of CART-A + and CART-A-alleles were 83.6% and 16.4% respectively. The frequencies of CART-A + A +, A + A-, A-A- genotype were 68.9%, 29.4% and 1.7% respectively. (2) In diabetic patients, the frequencies of CART-A + and A-alleles were 84.6% and 15.4% respectively; the frequencies of CART-A + A +, A + A-, A-A- genotype were 71.9%, 25.3% and 2.7% respectively. The frequency of A deletion of the CART gene in diabetic patients did not differ significantly from normoglycemic control subjects. (3) Diabetic patients with the A deletion had increased total cholesterol, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol. CONCLUSION: Polymorphism was found in the 3'-untranslated region (Delta A1457) of CART in Chinese. A deletion in CART is not associated with type 2 diabetes, but may contribute to dyslipidemia.

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