Association of the level of gγ chain in the fetal hemoglobin of normal adults with specific haplotypes

Y. Hattori, F. Kutlar, C. J. Mosley, S. M. Mayson, T. H.J. Huisman

Research output: Contribution to journalArticle

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Abstract

The levels of Gγ chain in the fetal hemoglobin of more than 40 Black and Caucasian females were determined with a sensitive high performance liquid chromatography procedure and were correlated with their haplotypes, defined by the presence or absence of 10 different restriction sites. Blood was collected during the 16th and 31st week of pregnancy because of a slightly elevated level of Hb F which facilitated the isolation of this protein from a relatively small sample. Four distinct Gγ levels were observed, each being associated with a specific haplotype. Homozygosity for sub-haplotype A [-+ +plus; +] is associated with high Gγ values (60-70%; that for sub-haplotype B [-ndash;plus;] with low levels (25-30%; and that for sub-haplotype C [+ndash;ndash;] with very low levels (10-15% (restriction sites listed are Hinc II at e; Xmn 1 5' to Gγ; Hind III at Gγ and Aγ; Hinc II at β and 3' to it). Sub-haplotype D [(14)-+ndash;plus;] with a rare polymorphism 5' is associated with extremely high Gγ values. Hb F levels were low (< 2.5% and were independent of the haplotype. It is speculated that, yet unknown, variations in the DNA of gene activity controlling regions are responsible for the differences in Gγ value.

Original languageEnglish (US)
Pages (from-to)185-204
Number of pages20
JournalHemoglobin
Volume10
Issue number2
DOIs
StatePublished - Jan 1 1986

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Fetal Hemoglobin
High performance liquid chromatography
Polymorphism
Haplotypes
Blood
Genes
DNA
Proteins
High Pressure Liquid Chromatography
Pregnancy

ASJC Scopus subject areas

  • Hematology
  • Clinical Biochemistry
  • Genetics(clinical)
  • Biochemistry, medical

Cite this

Hattori, Y., Kutlar, F., Mosley, C. J., Mayson, S. M., & Huisman, T. H. J. (1986). Association of the level of gγ chain in the fetal hemoglobin of normal adults with specific haplotypes. Hemoglobin, 10(2), 185-204. https://doi.org/10.3109/03630268609046444

Association of the level of gγ chain in the fetal hemoglobin of normal adults with specific haplotypes. / Hattori, Y.; Kutlar, F.; Mosley, C. J.; Mayson, S. M.; Huisman, T. H.J.

In: Hemoglobin, Vol. 10, No. 2, 01.01.1986, p. 185-204.

Research output: Contribution to journalArticle

Hattori, Y. ; Kutlar, F. ; Mosley, C. J. ; Mayson, S. M. ; Huisman, T. H.J. / Association of the level of gγ chain in the fetal hemoglobin of normal adults with specific haplotypes. In: Hemoglobin. 1986 ; Vol. 10, No. 2. pp. 185-204.
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