Astrocytes modulate retinal vasculogenesis: Effects on fibronectin expression

B. Jiang, Gregory I Liou, M. A. Behzadian, Ruth B Caldwell

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Vasculogenesis is the formation of blood-vessels by differentiation of vascular precursor cells. Experiments using retinal models were designed to test the hypothesis that astrocytes influence this process by effects on the composition of the extracellular matrix. Retinal vasculogenesis was studied in relation to the migration of astrocytes and expression of the extracellular matrix proteins laminin and fibronectin by in vivo experiments in neonatal rats. The results show that astrocytes spread into the retina just ahead of the newly formed vessels, where they probably initiate vasculogenesis. They also establish that fibronectin, but not laminin, is expressed in the zone of vasculogenesis immediately prior to vessel formation. Increased amounts of fibronectin mRNA indicate that fibronectin is synthesized by cells within this same region during this same time period. Later, as the new vessels form, differentiation of endothelial cells is correlated with the appearance of pericytes in the vessel wall and laminin in the vascular basement membrane. In vitro experiments using conditioned medium approaches showed that astrocytes stimulate endothelial cell fibronectin expression. Taken together with the in vivo observations these in vitro results suggest that fibronectin expression is an essential component in the initiation of retinal vasculogenesis. This study is the first indication that astrocytes influence the fibronectin component of the extracellular matrix during retinal vasculogenesis and that expression of fibronectin precedes that of laminin in this process.

Original languageEnglish (US)
Pages (from-to)2499-2508
Number of pages10
JournalJournal of Cell Science
Volume107
Issue number9
StatePublished - Sep 1 1994

Fingerprint

Fibronectins
Astrocytes
Laminin
Blood Vessels
Extracellular Matrix
Endothelial Cells
Pericytes
Extracellular Matrix Proteins
Conditioned Culture Medium
Basement Membrane
Retina
Messenger RNA

Keywords

  • Astrocyte
  • Fibronectin
  • Gene expression
  • In situ hybridization
  • Laminin
  • Retina
  • Vascular development

ASJC Scopus subject areas

  • Cell Biology

Cite this

Astrocytes modulate retinal vasculogenesis : Effects on fibronectin expression. / Jiang, B.; Liou, Gregory I; Behzadian, M. A.; Caldwell, Ruth B.

In: Journal of Cell Science, Vol. 107, No. 9, 01.09.1994, p. 2499-2508.

Research output: Contribution to journalArticle

Jiang, B, Liou, GI, Behzadian, MA & Caldwell, RB 1994, 'Astrocytes modulate retinal vasculogenesis: Effects on fibronectin expression', Journal of Cell Science, vol. 107, no. 9, pp. 2499-2508.
Jiang, B. ; Liou, Gregory I ; Behzadian, M. A. ; Caldwell, Ruth B. / Astrocytes modulate retinal vasculogenesis : Effects on fibronectin expression. In: Journal of Cell Science. 1994 ; Vol. 107, No. 9. pp. 2499-2508.
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