Asymmetric dimethylarginine induces endothelial nitric-oxide synthase mitochondrial redistribution through the nitration-mediated activation of Akt1

Ruslan Rafikov, Olga Rafikova, Saurabh Aggarwal, Christine Gross, Xutong Sun, Julin Desai, David J Fulton, Stephen Matthew Black

Research output: Contribution to journalArticle

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Abstract

We have recently demonstrated that asymmetric dimethylarginine (ADMA) induces the translocation of endothelial nitric-oxide synthase (eNOS) to the mitochondrion via a mechanism that requires protein nitration. Thus, the goal of this study was elucidate how eNOS redistributes to mitochondria and to identify the nitrated protein responsible for this event. Our data indicate that exposure of pulmonary arterial endothelial cells to ADMA enhanced eNOS phosphorylation at the Akt1-dependent phosphorylation sites Ser617 and Ser1179. Mutation of these serine residues to alanine (S617A and S1179A) inhibited nitration-mediated eNOS translocation to the mitochondria, whereas the phosphormimic mutations (S617D and S1179D) exhibited increased mitochondrial redistribution in the absence of ADMA. The overexpression of a dominant-negative Akt1 also attenuated ADMA-mediated eNOS mitochondrial translocation. Furthermore, ADMA enhanced Akt1 nitration and increased its activity. Mass spectrometry identified a single nitration site in Akt1 located at the tyrosine residue (Tyr350) located within the client-binding domain. Replacement of Tyr350 with phenylalanine abolished peroxynitrite-mediated eNOS translocation to mitochondria. We also found that in the absence of ADMA, eNOS translocation decreased mitochondrial oxygen consumption and superoxide production without altering cellular ATP level. This suggests that under physiologic conditions, eNOS translocation enhances mitochondria coupling. In conclusion, we have identified a new mechanism by which eNOS translocation to mitochondria is regulated by the phosphorylation of eNOS at Ser617 and Ser1179 by Akt1 and that this is enhanced when Akt1 becomes nitrated at Tyr350.

Original languageEnglish (US)
Pages (from-to)6212-6226
Number of pages15
JournalJournal of Biological Chemistry
Volume288
Issue number9
DOIs
StatePublished - Mar 1 2013

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Nitration
Nitric Oxide Synthase Type III
Chemical activation
Mitochondria
Phosphorylation
N,N-dimethylarginine
Mutation
Peroxynitrous Acid
Endothelial cells
Phenylalanine
Oxygen Consumption
Superoxides
Alanine
Serine
Mass spectrometry
Tyrosine
Mass Spectrometry
Proteins
Thermodynamic properties
Endothelial Cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Asymmetric dimethylarginine induces endothelial nitric-oxide synthase mitochondrial redistribution through the nitration-mediated activation of Akt1. / Rafikov, Ruslan; Rafikova, Olga; Aggarwal, Saurabh; Gross, Christine; Sun, Xutong; Desai, Julin; Fulton, David J; Black, Stephen Matthew.

In: Journal of Biological Chemistry, Vol. 288, No. 9, 01.03.2013, p. 6212-6226.

Research output: Contribution to journalArticle

Rafikov, Ruslan ; Rafikova, Olga ; Aggarwal, Saurabh ; Gross, Christine ; Sun, Xutong ; Desai, Julin ; Fulton, David J ; Black, Stephen Matthew. / Asymmetric dimethylarginine induces endothelial nitric-oxide synthase mitochondrial redistribution through the nitration-mediated activation of Akt1. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 9. pp. 6212-6226.
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