Ataxia telangiectasia and rad3-related overexpressing cancer cells induce prolonged G2 arrest and develop resistance to ionizing radiation

Young Mee Kim, Yeo Myoung Lee, Soo Yeon Park, Hongryull Pyo

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

We investigated whether ataxia telangiectasia and rad3-related (ATR) kinases regulate prolongation of ionizing radiation (IR) induced-G2 arrest and radioresistance in ataxia telangiectasia mutated-intact cancer cells. ATR overexpressing cancer cells showed prolonged-G2 arrest after IR exposure and were significantly resistant to DNA damaging stresses. The phosphorylation of p-Ser15-p53, p-Ser345-Chk1, and p-Tyr15-Cdk1 phosphorylation was increased until 36 h after IR exposure in ATR-overexpressing cells, whereas p-Ser10-histone H3 decreased. ATR-overexpressing cells also showed rapid attenuation of increased γ-H2AX foci after IR exposure compared with control cells. In contrast, ATR knockdown cells had limited clearance of γ-H2AX foci after IR exposure. In conclusion, ATR overexpression seems to primarily induce prolonged G2 arrest after IR exposure, which increases IR resistance by enhancing DNA damage repair. These results may provide useful clues for understanding the function of ATR in controlling IR-induced G2 arrest and radiation response.

Original languageEnglish (US)
Pages (from-to)219-227
Number of pages9
JournalDNA and cell biology
Volume30
Issue number4
DOIs
StatePublished - Apr 1 2011

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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