Atf3 deficiency promotes genome instability and spontaneous tumorigenesis in mice

Z. Wang, Yukai He, W. Deng, L. Lang, H. Yang, B. Jin, Ravindra Bharat Kolhe, Hanfei Ding, J. Zhang, T. Hai, Chunhong Yan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Mice lacking genes involving in the DNA-damage response (DDR) are often tumor prone owing to genome instability caused by oncogenic challenges. Previous studies demonstrate that activating transcription factor 3 (ATF3), a common stress sensor, can activate the tumor suppressor p53 and regulate expression of p53 target genes upon DNA damage. However, whether ATF3 contributes to the maintenance of genome stability and tumor suppression remains unknown. Here we report that Atf3-deficient (Atf3 ') mice developed spontaneous tumors, and died significantly earlier than wild-type (Atf3 +/+) mice. Consistent with these results, Atf3 ' mouse embryonic fibroblasts (MEFs) had more aberrant chromosomes and micronuclei, and were genetically unstable. Whereas we demonstrated that ATF3 activated p53 and promoted its pro-apoptotic activity in mouse thymi and small intestines, the chromosomal instability caused by Atf3 deficiency was largely dependent on the regulation of p53 by ATF3. Interestingly, loss of Atf3 also promoted spontaneous tumorigenesis in Trp53 +/ ' mice, but did not affect tumor formation in Trp53 /' mice. Our results thus provide the first genetic evidence linking ATF3 to the suppression of the early development of cancer, and underscore the importance of ATF3 in the maintenance of genome integrity.

Original languageEnglish (US)
Pages (from-to)18-27
Number of pages10
JournalOncogene
Volume37
Issue number1
DOIs
StatePublished - Jan 4 2018

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Activating Transcription Factor 3
Genomic Instability
Carcinogenesis
Neoplasms
DNA Damage
Maintenance
Chromosomal Instability
p53 Genes
Thymus Gland
Small Intestine
Fibroblasts
Chromosomes
Genome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Atf3 deficiency promotes genome instability and spontaneous tumorigenesis in mice. / Wang, Z.; He, Yukai; Deng, W.; Lang, L.; Yang, H.; Jin, B.; Kolhe, Ravindra Bharat; Ding, Hanfei; Zhang, J.; Hai, T.; Yan, Chunhong.

In: Oncogene, Vol. 37, No. 1, 04.01.2018, p. 18-27.

Research output: Contribution to journalArticle

Wang, Z. ; He, Yukai ; Deng, W. ; Lang, L. ; Yang, H. ; Jin, B. ; Kolhe, Ravindra Bharat ; Ding, Hanfei ; Zhang, J. ; Hai, T. ; Yan, Chunhong. / Atf3 deficiency promotes genome instability and spontaneous tumorigenesis in mice. In: Oncogene. 2018 ; Vol. 37, No. 1. pp. 18-27.
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