ATP-Driven Exchange of Histone H2AZ Variant Catalyzed by SWR1 Chromatin Remodeling Complex

Gaku Mizuguchi, Xuetong Shen, Joe Landry, Wei-Hua Wu, Subhojit Sen, Carl Wu

    Research output: Contribution to journalArticle

    842 Citations (Scopus)

    Abstract

    The conserved histone variant H2AZ has an important role in the regulation of gene expression and the establishment of a buffer to the spread of silent heterochromatin. How histone variants such as H2AZ are incorporated into nucleosomes has been obscure. We have found that Swr1, a Swi2/Snf2-related adenosine triphosphatase, is the catalytic core of a multisubunit, histone-variant exchanger that efficiently replaces conventional histone H2A with histone H2AZ in nucleosome arrays. Swr1 is required for the deposition of histone H2AZ at specific chromosome locations in vivo, and Swr1 and H2AZ commonly regulate a subset of yeast genes. These findings define a previously unknown role for the adenosine triphosphate-dependent chromatin remodeling machinery.

    Original languageEnglish (US)
    Pages (from-to)343-348
    Number of pages6
    JournalScience
    Volume303
    Issue number5656
    DOIs
    StatePublished - Jan 16 2004

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    Chromatin Assembly and Disassembly
    Histones
    Adenosine Triphosphate
    Nucleosomes
    Heterochromatin
    Gene Expression Regulation
    Adenosine Triphosphatases
    Catalytic Domain
    Buffers
    Chromosomes
    Yeasts
    Genes

    ASJC Scopus subject areas

    • General

    Cite this

    Mizuguchi, G., Shen, X., Landry, J., Wu, W-H., Sen, S., & Wu, C. (2004). ATP-Driven Exchange of Histone H2AZ Variant Catalyzed by SWR1 Chromatin Remodeling Complex. Science, 303(5656), 343-348. https://doi.org/10.1126/science.1090701

    ATP-Driven Exchange of Histone H2AZ Variant Catalyzed by SWR1 Chromatin Remodeling Complex. / Mizuguchi, Gaku; Shen, Xuetong; Landry, Joe; Wu, Wei-Hua; Sen, Subhojit; Wu, Carl.

    In: Science, Vol. 303, No. 5656, 16.01.2004, p. 343-348.

    Research output: Contribution to journalArticle

    Mizuguchi, G, Shen, X, Landry, J, Wu, W-H, Sen, S & Wu, C 2004, 'ATP-Driven Exchange of Histone H2AZ Variant Catalyzed by SWR1 Chromatin Remodeling Complex', Science, vol. 303, no. 5656, pp. 343-348. https://doi.org/10.1126/science.1090701
    Mizuguchi, Gaku ; Shen, Xuetong ; Landry, Joe ; Wu, Wei-Hua ; Sen, Subhojit ; Wu, Carl. / ATP-Driven Exchange of Histone H2AZ Variant Catalyzed by SWR1 Chromatin Remodeling Complex. In: Science. 2004 ; Vol. 303, No. 5656. pp. 343-348.
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