Attenuation of oxidative damage-associated cognitive decline by Withania somnifera in rat model of streptozotocin-induced cognitive impairment

Md Ejaz Ahmed, Hayate Javed, Mohd Moshahid Khan, Kumar Vaibhav, Ajmal Ahmad, Andleeb Khan, Rizwana Tabassum, Farah Islam, Mohammed M. Safhi, Fakhrul Islam

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Oxidative stress is a critical contributing factor to age-related neurodegenerative disorders. Therefore, the inhibition of oxidative damage, responsible for chronic detrimental neurodegeneration, is an important strategy for neuroprotective therapy. Withania somnifera (WS) extract has been reported to have potent antioxidant and free radical quenching properties in various disease conditions. The present study evaluated the hypothesis that WS extract would reduce oxidative stress-associated neurodegeneration after intracerebroventricular injection of streptozotocin (ICV-STZ) in rats. To test this hypothesis, male Wistar rats were pretreated with WS extract at doses of 100, 200, and 300 mg/kg body weight once daily for 3 weeks. On day 22nd, the rats were infused bilaterally with ICV-STZ injection (3 mg/kg body weight) in normal saline while sham group received only saline. Two weeks after the lesioning, STZ-infused rats showed cognitive impairment in the Morris water maze test. The rats were sacrificed after 3 weeks of the lesioning for the estimation of the contents of lipid peroxidation, reduced glutathione, and activities of glutathione reductase, glutathione peroxidase, and catalase. Pretreatment with WS extract attenuated behavioral, biochemical, and histological alterations significantly in dose-dependent manner in the hippocampus and cerebral cortex of ICV-STZ-infused rats. These results suggest that WS affords a beneficial effect on cognitive deficit by ameliorating oxidative damage induced by streptozotocin in a model of cognitive impairment.

Original languageEnglish (US)
Pages (from-to)1067-1078
Number of pages12
JournalProtoplasma
Volume250
Issue number5
DOIs
StatePublished - Jan 24 2013

Fingerprint

Withania
Withania somnifera
streptozotocin
Streptozocin
animal models
rats
Injections
injection
extracts
Oxidative Stress
oxidative stress
Ideal Body Weight
Glutathione Reductase
Age Factors
Glutathione Peroxidase
body weight
Neurodegenerative Diseases
Cerebral Cortex
cerebral cortex
Catalase

Keywords

  • Antioxidant
  • Cognitive impairment
  • Oxidative stress
  • Streptozotocin
  • Withania somnifera

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Attenuation of oxidative damage-associated cognitive decline by Withania somnifera in rat model of streptozotocin-induced cognitive impairment. / Ahmed, Md Ejaz; Javed, Hayate; Khan, Mohd Moshahid; Vaibhav, Kumar; Ahmad, Ajmal; Khan, Andleeb; Tabassum, Rizwana; Islam, Farah; Safhi, Mohammed M.; Islam, Fakhrul.

In: Protoplasma, Vol. 250, No. 5, 24.01.2013, p. 1067-1078.

Research output: Contribution to journalArticle

Ahmed, ME, Javed, H, Khan, MM, Vaibhav, K, Ahmad, A, Khan, A, Tabassum, R, Islam, F, Safhi, MM & Islam, F 2013, 'Attenuation of oxidative damage-associated cognitive decline by Withania somnifera in rat model of streptozotocin-induced cognitive impairment', Protoplasma, vol. 250, no. 5, pp. 1067-1078. https://doi.org/10.1007/s00709-013-0482-2
Ahmed, Md Ejaz ; Javed, Hayate ; Khan, Mohd Moshahid ; Vaibhav, Kumar ; Ahmad, Ajmal ; Khan, Andleeb ; Tabassum, Rizwana ; Islam, Farah ; Safhi, Mohammed M. ; Islam, Fakhrul. / Attenuation of oxidative damage-associated cognitive decline by Withania somnifera in rat model of streptozotocin-induced cognitive impairment. In: Protoplasma. 2013 ; Vol. 250, No. 5. pp. 1067-1078.
@article{241a618a0780428cbc1dc133b9ab09ea,
title = "Attenuation of oxidative damage-associated cognitive decline by Withania somnifera in rat model of streptozotocin-induced cognitive impairment",
abstract = "Oxidative stress is a critical contributing factor to age-related neurodegenerative disorders. Therefore, the inhibition of oxidative damage, responsible for chronic detrimental neurodegeneration, is an important strategy for neuroprotective therapy. Withania somnifera (WS) extract has been reported to have potent antioxidant and free radical quenching properties in various disease conditions. The present study evaluated the hypothesis that WS extract would reduce oxidative stress-associated neurodegeneration after intracerebroventricular injection of streptozotocin (ICV-STZ) in rats. To test this hypothesis, male Wistar rats were pretreated with WS extract at doses of 100, 200, and 300 mg/kg body weight once daily for 3 weeks. On day 22nd, the rats were infused bilaterally with ICV-STZ injection (3 mg/kg body weight) in normal saline while sham group received only saline. Two weeks after the lesioning, STZ-infused rats showed cognitive impairment in the Morris water maze test. The rats were sacrificed after 3 weeks of the lesioning for the estimation of the contents of lipid peroxidation, reduced glutathione, and activities of glutathione reductase, glutathione peroxidase, and catalase. Pretreatment with WS extract attenuated behavioral, biochemical, and histological alterations significantly in dose-dependent manner in the hippocampus and cerebral cortex of ICV-STZ-infused rats. These results suggest that WS affords a beneficial effect on cognitive deficit by ameliorating oxidative damage induced by streptozotocin in a model of cognitive impairment.",
keywords = "Antioxidant, Cognitive impairment, Oxidative stress, Streptozotocin, Withania somnifera",
author = "Ahmed, {Md Ejaz} and Hayate Javed and Khan, {Mohd Moshahid} and Kumar Vaibhav and Ajmal Ahmad and Andleeb Khan and Rizwana Tabassum and Farah Islam and Safhi, {Mohammed M.} and Fakhrul Islam",
year = "2013",
month = "1",
day = "24",
doi = "10.1007/s00709-013-0482-2",
language = "English (US)",
volume = "250",
pages = "1067--1078",
journal = "Protoplasma",
issn = "0033-183X",
publisher = "Springer Wien",
number = "5",

}

TY - JOUR

T1 - Attenuation of oxidative damage-associated cognitive decline by Withania somnifera in rat model of streptozotocin-induced cognitive impairment

AU - Ahmed, Md Ejaz

AU - Javed, Hayate

AU - Khan, Mohd Moshahid

AU - Vaibhav, Kumar

AU - Ahmad, Ajmal

AU - Khan, Andleeb

AU - Tabassum, Rizwana

AU - Islam, Farah

AU - Safhi, Mohammed M.

AU - Islam, Fakhrul

PY - 2013/1/24

Y1 - 2013/1/24

N2 - Oxidative stress is a critical contributing factor to age-related neurodegenerative disorders. Therefore, the inhibition of oxidative damage, responsible for chronic detrimental neurodegeneration, is an important strategy for neuroprotective therapy. Withania somnifera (WS) extract has been reported to have potent antioxidant and free radical quenching properties in various disease conditions. The present study evaluated the hypothesis that WS extract would reduce oxidative stress-associated neurodegeneration after intracerebroventricular injection of streptozotocin (ICV-STZ) in rats. To test this hypothesis, male Wistar rats were pretreated with WS extract at doses of 100, 200, and 300 mg/kg body weight once daily for 3 weeks. On day 22nd, the rats were infused bilaterally with ICV-STZ injection (3 mg/kg body weight) in normal saline while sham group received only saline. Two weeks after the lesioning, STZ-infused rats showed cognitive impairment in the Morris water maze test. The rats were sacrificed after 3 weeks of the lesioning for the estimation of the contents of lipid peroxidation, reduced glutathione, and activities of glutathione reductase, glutathione peroxidase, and catalase. Pretreatment with WS extract attenuated behavioral, biochemical, and histological alterations significantly in dose-dependent manner in the hippocampus and cerebral cortex of ICV-STZ-infused rats. These results suggest that WS affords a beneficial effect on cognitive deficit by ameliorating oxidative damage induced by streptozotocin in a model of cognitive impairment.

AB - Oxidative stress is a critical contributing factor to age-related neurodegenerative disorders. Therefore, the inhibition of oxidative damage, responsible for chronic detrimental neurodegeneration, is an important strategy for neuroprotective therapy. Withania somnifera (WS) extract has been reported to have potent antioxidant and free radical quenching properties in various disease conditions. The present study evaluated the hypothesis that WS extract would reduce oxidative stress-associated neurodegeneration after intracerebroventricular injection of streptozotocin (ICV-STZ) in rats. To test this hypothesis, male Wistar rats were pretreated with WS extract at doses of 100, 200, and 300 mg/kg body weight once daily for 3 weeks. On day 22nd, the rats were infused bilaterally with ICV-STZ injection (3 mg/kg body weight) in normal saline while sham group received only saline. Two weeks after the lesioning, STZ-infused rats showed cognitive impairment in the Morris water maze test. The rats were sacrificed after 3 weeks of the lesioning for the estimation of the contents of lipid peroxidation, reduced glutathione, and activities of glutathione reductase, glutathione peroxidase, and catalase. Pretreatment with WS extract attenuated behavioral, biochemical, and histological alterations significantly in dose-dependent manner in the hippocampus and cerebral cortex of ICV-STZ-infused rats. These results suggest that WS affords a beneficial effect on cognitive deficit by ameliorating oxidative damage induced by streptozotocin in a model of cognitive impairment.

KW - Antioxidant

KW - Cognitive impairment

KW - Oxidative stress

KW - Streptozotocin

KW - Withania somnifera

UR - http://www.scopus.com/inward/record.url?scp=84884907231&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884907231&partnerID=8YFLogxK

U2 - 10.1007/s00709-013-0482-2

DO - 10.1007/s00709-013-0482-2

M3 - Article

C2 - 23340606

AN - SCOPUS:84884907231

VL - 250

SP - 1067

EP - 1078

JO - Protoplasma

JF - Protoplasma

SN - 0033-183X

IS - 5

ER -