Atypical roles for Campylobacter jejuni amino acid ATP binding cassette transporter components PaqP and PaqQ in bacterial stress tolerance and pathogen-host cell dynamics

Ann E. Lin, Kirsten Krastel, Rhonda I. Hobb, Stuart A Thompson, Dennis G. Cvitkovitch, Erin C. Gaynor

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Campylobacter jejuni is a human pathogen causing severe diarrheal disease; however, our understanding of the survival of C. jejuni during disease and transmission remains limited. Amino acid ATP binding cassette (AA-ABC) transporters in C. jejuni have been proposed as important pathogenesis factors. We have investigated a novel AA-ABC transporter system, encoded by cj0467 to cj0469, by generating targeted deletions of cj0467 (the membrane transport component) and cj0469 (the ATPase component) in C. jejuni 81-176. The analyses described here have led us to designate these genes paqP and paqQ, respectively (pathogenesis-associated glutamine [q] ABC transporter permease [P] and ATPase [Q]). We found that loss of either component resulted in amino acid uptake defects, most notably diminished glutamine uptake. Altered resistance to a series of environmental and in vivo stresses was also observed: both mutants were hyperresistant to aerobic and organic peroxide stress, and while the ΔpaqP mutant was also hyperresistant to heat and osmotic shock, the ΔpaqQ mutant was more susceptible than the wild type to the latter two stresses. The ΔpaqP and ΔpaqQ mutants also displayed a surprising but statistically significant increase in recovery from macrophages and epithelial cells in short-term intracellular survival assays. Annexin V, 4′,6-diamidino-2-phenylindole (DAPI), and Western blot analyses revealed that macrophages infected with the ΔpaqP or ΔpaqQ mutant exhibited transient but significant decreases in cell death and extracellular signal-regulated kinase-mitogen-activated protein kinase activation compared to levels in wild-type-infected cells. The ΔpaqP mutant was not defective in either short-term or longer-term mouse colonization, consistent with its increased stress survival and diminished host cell damage phenotypes. Collectively, these results demonstrate a unique correlation of an AA-ABC transporter with bacterial stress tolerances and host cell responses to pathogen infection.

Original languageEnglish (US)
Pages (from-to)4912-4924
Number of pages13
JournalInfection and Immunity
Volume77
Issue number11
DOIs
StatePublished - Nov 1 2009

Fingerprint

Campylobacter jejuni
ATP-Binding Cassette Transporters
Amino Acids
Glutamine
Adenosine Triphosphatases
Macrophages
Membrane Transport Proteins
Survival
Annexin A5
Extracellular Signal-Regulated MAP Kinases
Osmotic Pressure
Peroxides
Mitogen-Activated Protein Kinases
Cell Death
Hot Temperature
Western Blotting
Epithelial Cells
Phenotype
Membranes
Infection

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Atypical roles for Campylobacter jejuni amino acid ATP binding cassette transporter components PaqP and PaqQ in bacterial stress tolerance and pathogen-host cell dynamics. / Lin, Ann E.; Krastel, Kirsten; Hobb, Rhonda I.; Thompson, Stuart A; Cvitkovitch, Dennis G.; Gaynor, Erin C.

In: Infection and Immunity, Vol. 77, No. 11, 01.11.2009, p. 4912-4924.

Research output: Contribution to journalArticle

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abstract = "Campylobacter jejuni is a human pathogen causing severe diarrheal disease; however, our understanding of the survival of C. jejuni during disease and transmission remains limited. Amino acid ATP binding cassette (AA-ABC) transporters in C. jejuni have been proposed as important pathogenesis factors. We have investigated a novel AA-ABC transporter system, encoded by cj0467 to cj0469, by generating targeted deletions of cj0467 (the membrane transport component) and cj0469 (the ATPase component) in C. jejuni 81-176. The analyses described here have led us to designate these genes paqP and paqQ, respectively (pathogenesis-associated glutamine [q] ABC transporter permease [P] and ATPase [Q]). We found that loss of either component resulted in amino acid uptake defects, most notably diminished glutamine uptake. Altered resistance to a series of environmental and in vivo stresses was also observed: both mutants were hyperresistant to aerobic and organic peroxide stress, and while the ΔpaqP mutant was also hyperresistant to heat and osmotic shock, the ΔpaqQ mutant was more susceptible than the wild type to the latter two stresses. The ΔpaqP and ΔpaqQ mutants also displayed a surprising but statistically significant increase in recovery from macrophages and epithelial cells in short-term intracellular survival assays. Annexin V, 4′,6-diamidino-2-phenylindole (DAPI), and Western blot analyses revealed that macrophages infected with the ΔpaqP or ΔpaqQ mutant exhibited transient but significant decreases in cell death and extracellular signal-regulated kinase-mitogen-activated protein kinase activation compared to levels in wild-type-infected cells. The ΔpaqP mutant was not defective in either short-term or longer-term mouse colonization, consistent with its increased stress survival and diminished host cell damage phenotypes. Collectively, these results demonstrate a unique correlation of an AA-ABC transporter with bacterial stress tolerances and host cell responses to pathogen infection.",
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