Autocrine-paracrine inhibition of growth hormone and prolactin production by GH3 cell-conditioned medium

C. A. Lapp, J. M. Tyler, Y. S. Lee, M. E. Stachura

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In previous work we have shown that perifused GH3 cells exhibit spontaneously accelerating growth hormone (GH) and prolactin (PRL) secretory rates. This behavior contrasts with GH and PRL secretion rates that are decreasing or stable over the same 3-d period in static cell culture. We now report that GH3 cells maintained in serum-supplemented medium produce an autocrine-paracrine factor(s) which inhibits GH secretion in plate culture; PRL release is frequently reduced as well. The inhibitory effect of conditioned medium on GH secretion was concentration dependent, whereas PRL release was stimulated at low and inhibited at high concentrations over the same range. Extensive dialysis of conditioned medium using membranes with a molecular weight cut-off of 12 000-14 000 did not remove GH inhibition but produced a retentate that stimulated PRL secretion. Heat-inactivation of conditioned medium did not abolish inhibition of GH release but did remove the PRL-stimulatory effect. IGF-I added to fresh culture medium did not reproduce the GH-inhibitory effects of conditioned medium. We conclude that GH3 cell secretory behavior in perifusion and plate culture systems may be partially explained by the production of an autocrine-paracrine factor: its accumulation in plate culture inhibits GH and PRL secretion whereas its removal, by perifusing medium, allows GH and PRL secretion to accelerate.

Original languageEnglish (US)
Pages (from-to)528-534
Number of pages7
JournalIn Vitro Cellular & Developmental Biology
Volume25
Issue number6
DOIs
StatePublished - Jun 1 1989

Fingerprint

Conditioned Culture Medium
Prolactin
Growth Hormone
Secretory Rate
Dialysis
Insulin-Like Growth Factor I
Cell culture
Culture Media
Cell Culture Techniques
Hot Temperature
Molecular Weight
Molecular weight
Membranes
Serum

Keywords

  • GH cells
  • autocrine
  • conditioned medium
  • growth hormone (GH)
  • paracrine
  • perifusion
  • prolactin (PRL)

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Autocrine-paracrine inhibition of growth hormone and prolactin production by GH3 cell-conditioned medium. / Lapp, C. A.; Tyler, J. M.; Lee, Y. S.; Stachura, M. E.

In: In Vitro Cellular & Developmental Biology, Vol. 25, No. 6, 01.06.1989, p. 528-534.

Research output: Contribution to journalArticle

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