An improved method is described for the detection of ganglioside antigens with the antibodies on tbin-layer.chromatograms. The method involves the following steps: Separation of gangliosides on a high-performance thin-layer chrornatographic plate, application of antibody solution, and detection of bound antibody with staphy-lococcal protein A. Using specific antibodies against gangliosides GM4, GM1, GD3, and asialo GM1 ganglioside, the method allowed positive identification of these antigens on thin-layer plates. It also provides a convenient means of assessing the specificity of an ant i-glycolipid antibody.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of Biochemistry|
|State||Published - 1984|
ASJC Scopus subject areas
- Molecular Biology