Autoradiography of ganglioside antigens separated by high-performance. Thin-layer chromatography vith their antibodies

Noriyuki Kasai, Masaharu Naiki, Robert K. Yu

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

An improved method is described for the detection of ganglioside antigens with the antibodies on tbin-layer.chromatograms. The method involves the following steps: Separation of gangliosides on a high-performance thin-layer chrornatographic plate, application of antibody solution, and detection of bound antibody with [125]staphy-lococcal protein A. Using specific antibodies against gangliosides GM4, GM1, GD3, and asialo GM1 ganglioside, the method allowed positive identification of these antigens on thin-layer plates. It also provides a convenient means of assessing the specificity of an ant i-glycolipid antibody.

Original languageEnglish (US)
Pages (from-to)261-264
Number of pages4
JournalJournal of Biochemistry
Volume96
Issue number1
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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