Azadirachta indica mitigates behavioral impairments, oxidative damage, histological alterations and apoptosis in focal cerebral ischemia-reperfusion model of rats

Kumar Vaibhav; Pallavi Shrivastava; Andleeb Khan; Hayate Javed; Rizwana Tabassum; Md Ejaz Ahmed; Mohammad Badruzzaman Khan; Fakhrul Islam; Mohd Moshahid Khan; Farah Islam; Sayeed Ahmad; M. Saeed Siddiqui; Mohammed M. Safhi

doi:10.1007/s10072-012-1238-z

Azadirachta indica mitigates behavioral impairments, oxidative damage, histological alterations and apoptosis in focal cerebral ischemia-reperfusion model of rats

Kumar Vaibhav, Pallavi Shrivastava, Andleeb Khan, Hayate Javed, Rizwana Tabassum, Md Ejaz Ahmed, Mohammad Badruzzaman Khan, Fakhrul Islam, Mohd Moshahid Khan, Farah Islam, Sayeed Ahmad, M. Saeed Siddiqui, Mohammed M. Safhi

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Azadirachta indica Linn. (Meliaceae) has been used from ancient times as a remedy for various ailments. The present study was designed to investigate the antioxidant and anti-apoptotic properties of A. indica seed extract (ASE) in transient middle cerebral artery occlusion (MCAO) rat model. Antioxidant potential of ASE was determined in vitro. Further, ASE was evaluated against neurological deficits, histological alterations (TTC, CV and H&E) and oxidative damage (TBARS, GSH and nitrite) in MCAO rats. Moreover, caspase-3 and -9 were analyzed to evaluate the anti-apoptotic activity of ASE. ASE has shown potent in vitro reducing power (126.2 mg AsAE/g extract) and free radical scavenging activities (DPPH 171.0 and NO 176.0 μg/ml). Furthermore, ASE inhibited oxidative stress and decreased the activities of caspase-3 (26.7 %, p < 0.05) and caspase-9 (31.2 %, p < 0.01) thus, reduced neuronal loss in MCAO rats. Our data revealed that ASE has potent antioxidant and anti-apoptotic properties, and may be explored for its active constituents against neurodegenerative diseases.

Original language	English (US)
Pages (from-to)	1321-1330
Number of pages	10
Journal	Neurological Sciences
Volume	34
Issue number	8
DOIs	https://doi.org/10.1007/s10072-012-1238-z
State	Published - Aug 2013

Keywords

Antioxidant
Azadirachta indica
Behavioral deficit
Caspase-3
Oxidative stress

ASJC Scopus subject areas

Dermatology
Clinical Neurology
Psychiatry and Mental health

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10072-012-1238-z

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Vaibhav, K., Shrivastava, P., Khan, A., Javed, H., Tabassum, R., Ahmed, M. E., Khan, M. B., Islam, F., Moshahid Khan, M., Islam, F., Ahmad, S., Siddiqui, M. S., & Safhi, M. M. (2013). Azadirachta indica mitigates behavioral impairments, oxidative damage, histological alterations and apoptosis in focal cerebral ischemia-reperfusion model of rats. Neurological Sciences, 34(8), 1321-1330. https://doi.org/10.1007/s10072-012-1238-z

Vaibhav, K, Shrivastava, P, Khan, A, Javed, H, Tabassum, R, Ahmed, ME, Khan, MB, Islam, F, Moshahid Khan, M, Islam, F, Ahmad, S, Siddiqui, MS & Safhi, MM 2013, 'Azadirachta indica mitigates behavioral impairments, oxidative damage, histological alterations and apoptosis in focal cerebral ischemia-reperfusion model of rats', Neurological Sciences, vol. 34, no. 8, pp. 1321-1330. https://doi.org/10.1007/s10072-012-1238-z

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abstract = "Azadirachta indica Linn. (Meliaceae) has been used from ancient times as a remedy for various ailments. The present study was designed to investigate the antioxidant and anti-apoptotic properties of A. indica seed extract (ASE) in transient middle cerebral artery occlusion (MCAO) rat model. Antioxidant potential of ASE was determined in vitro. Further, ASE was evaluated against neurological deficits, histological alterations (TTC, CV and H&E) and oxidative damage (TBARS, GSH and nitrite) in MCAO rats. Moreover, caspase-3 and -9 were analyzed to evaluate the anti-apoptotic activity of ASE. ASE has shown potent in vitro reducing power (126.2 mg AsAE/g extract) and free radical scavenging activities (DPPH 171.0 and NO 176.0 μg/ml). Furthermore, ASE inhibited oxidative stress and decreased the activities of caspase-3 (26.7 %, p < 0.05) and caspase-9 (31.2 %, p < 0.01) thus, reduced neuronal loss in MCAO rats. Our data revealed that ASE has potent antioxidant and anti-apoptotic properties, and may be explored for its active constituents against neurodegenerative diseases.",

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AU - Vaibhav, Kumar

AU - Shrivastava, Pallavi

AU - Khan, Andleeb

AU - Javed, Hayate

AU - Tabassum, Rizwana

AU - Ahmed, Md Ejaz

AU - Khan, Mohammad Badruzzaman

AU - Islam, Fakhrul

AU - Moshahid Khan, Mohd

AU - Islam, Farah

AU - Ahmad, Sayeed

AU - Siddiqui, M. Saeed

AU - Safhi, Mohammed M.

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AB - Azadirachta indica Linn. (Meliaceae) has been used from ancient times as a remedy for various ailments. The present study was designed to investigate the antioxidant and anti-apoptotic properties of A. indica seed extract (ASE) in transient middle cerebral artery occlusion (MCAO) rat model. Antioxidant potential of ASE was determined in vitro. Further, ASE was evaluated against neurological deficits, histological alterations (TTC, CV and H&E) and oxidative damage (TBARS, GSH and nitrite) in MCAO rats. Moreover, caspase-3 and -9 were analyzed to evaluate the anti-apoptotic activity of ASE. ASE has shown potent in vitro reducing power (126.2 mg AsAE/g extract) and free radical scavenging activities (DPPH 171.0 and NO 176.0 μg/ml). Furthermore, ASE inhibited oxidative stress and decreased the activities of caspase-3 (26.7 %, p < 0.05) and caspase-9 (31.2 %, p < 0.01) thus, reduced neuronal loss in MCAO rats. Our data revealed that ASE has potent antioxidant and anti-apoptotic properties, and may be explored for its active constituents against neurodegenerative diseases.

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KW - Azadirachta indica

KW - Behavioral deficit

KW - Caspase-3

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Azadirachta indica mitigates behavioral impairments, oxidative damage, histological alterations and apoptosis in focal cerebral ischemia-reperfusion model of rats

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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