Azadirachta indica mitigates behavioral impairments, oxidative damage, histological alterations and apoptosis in focal cerebral ischemia-reperfusion model of rats

Kumar Vaibhav, Pallavi Shrivastava, Andleeb Khan, Hayate Javed, Rizwana Tabassum, Md Ejaz Ahmed, Mohammad Badruzzaman Khan, Fakhrul Islam, Mohd Moshahid Khan, Farah Islam, Sayeed Ahmad, M. Saeed Siddiqui, Mohammed M. Safhi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Azadirachta indica Linn. (Meliaceae) has been used from ancient times as a remedy for various ailments. The present study was designed to investigate the antioxidant and anti-apoptotic properties of A. indica seed extract (ASE) in transient middle cerebral artery occlusion (MCAO) rat model. Antioxidant potential of ASE was determined in vitro. Further, ASE was evaluated against neurological deficits, histological alterations (TTC, CV and H&E) and oxidative damage (TBARS, GSH and nitrite) in MCAO rats. Moreover, caspase-3 and -9 were analyzed to evaluate the anti-apoptotic activity of ASE. ASE has shown potent in vitro reducing power (126.2 mg AsAE/g extract) and free radical scavenging activities (DPPH 171.0 and NO 176.0 μg/ml). Furthermore, ASE inhibited oxidative stress and decreased the activities of caspase-3 (26.7 %, p < 0.05) and caspase-9 (31.2 %, p < 0.01) thus, reduced neuronal loss in MCAO rats. Our data revealed that ASE has potent antioxidant and anti-apoptotic properties, and may be explored for its active constituents against neurodegenerative diseases.

Original languageEnglish (US)
Pages (from-to)1321-1330
Number of pages10
JournalNeurological Sciences
Volume34
Issue number8
DOIs
StatePublished - Aug 1 2013

Fingerprint

Azadirachta
Brain Ischemia
Reperfusion
Seeds
Apoptosis
Middle Cerebral Artery Infarction
Caspase 9
Antioxidants
Caspase 3
Meliaceae
Nitrites
Neurodegenerative Diseases
Free Radicals
Oxidative Stress

Keywords

  • Antioxidant
  • Azadirachta indica
  • Behavioral deficit
  • Caspase-3
  • Oxidative stress

ASJC Scopus subject areas

  • Dermatology
  • Clinical Neurology
  • Psychiatry and Mental health

Cite this

Azadirachta indica mitigates behavioral impairments, oxidative damage, histological alterations and apoptosis in focal cerebral ischemia-reperfusion model of rats. / Vaibhav, Kumar; Shrivastava, Pallavi; Khan, Andleeb; Javed, Hayate; Tabassum, Rizwana; Ahmed, Md Ejaz; Khan, Mohammad Badruzzaman; Islam, Fakhrul; Moshahid Khan, Mohd; Islam, Farah; Ahmad, Sayeed; Siddiqui, M. Saeed; Safhi, Mohammed M.

In: Neurological Sciences, Vol. 34, No. 8, 01.08.2013, p. 1321-1330.

Research output: Contribution to journalArticle

Vaibhav, K, Shrivastava, P, Khan, A, Javed, H, Tabassum, R, Ahmed, ME, Khan, MB, Islam, F, Moshahid Khan, M, Islam, F, Ahmad, S, Siddiqui, MS & Safhi, MM 2013, 'Azadirachta indica mitigates behavioral impairments, oxidative damage, histological alterations and apoptosis in focal cerebral ischemia-reperfusion model of rats', Neurological Sciences, vol. 34, no. 8, pp. 1321-1330. https://doi.org/10.1007/s10072-012-1238-z
Vaibhav, Kumar ; Shrivastava, Pallavi ; Khan, Andleeb ; Javed, Hayate ; Tabassum, Rizwana ; Ahmed, Md Ejaz ; Khan, Mohammad Badruzzaman ; Islam, Fakhrul ; Moshahid Khan, Mohd ; Islam, Farah ; Ahmad, Sayeed ; Siddiqui, M. Saeed ; Safhi, Mohammed M. / Azadirachta indica mitigates behavioral impairments, oxidative damage, histological alterations and apoptosis in focal cerebral ischemia-reperfusion model of rats. In: Neurological Sciences. 2013 ; Vol. 34, No. 8. pp. 1321-1330.
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