Bacterial chemoattractant synergizes with LPS to induce inflammation via upregulating TLR4

Yong Jiang, Laura Smith, Warren W. Pan, Xiaohuan Chen, Juan Wang, Xia Ying Lu, Shuang Huang, Zhixing K. Pan

Research output: Contribution to journalArticle

Abstract

The principal challenge for the host is to efficiently detect the invading pathogen and mount a rapid defensive response. Leukocytes recognize invading pathogens by directly interacting with pathogen-associated molecular patterns via Toll-like receptors (TLRs) expressed on the leukocyte surfaces. In the present study we show for the first time that fMLP synergized with LPS to induce an inflammatory response via up-regulation of TLR4 in vitro and in vivo. Moreover, we show that fMLP-induced TLR4 up-regulation was mediated by FPR and Gi/Go class of heterotrimeric G proteins. Interestingly, low molecular weight GTPase Rho A acted as a regulator for FPR-mediated TLR4 induction, thereby implying a critical role for Rho A GTPase in inflammatory response during bacterial infection. Our data indicate a possible important role of fMLP that bacterial chemoattractant fMLP not only attracts leukocytes but also contributes directly to inflammation by synergizing with bacterial LPS.

Original languageEnglish (US)
Pages (from-to)6005-6013
Number of pages9
JournalInternational Journal of Clinical and Experimental Medicine
Volume9
Issue number3
StatePublished - Mar 30 2016

Keywords

  • Bacterial chemoattractant
  • Gene expression
  • Inflammation
  • Receptor
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Jiang, Y., Smith, L., Pan, W. W., Chen, X., Wang, J., Lu, X. Y., Huang, S., & Pan, Z. K. (2016). Bacterial chemoattractant synergizes with LPS to induce inflammation via upregulating TLR4. International Journal of Clinical and Experimental Medicine, 9(3), 6005-6013.