BAPTI and BAPTISM birthdating of neurons in zebrafish

Y. Albert Pan, Sophie J.C. Caron, Alexander F. Schier

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

This protocol describes how the photoconvertible protein Kaede can be used to determine the birthdates of neurons in live zebrafish. The methods used are birthdating analysis by photoconverted fluorescent protein tracing in vivo (BAPTI) and BAPTI combined with subpopulation markers (BAPTISM). Because Kaede can be converted from green to red fluorescence at any developmental time point, it serves as a temporal landmark for cell birth. When it is used in combination with subpopulation markers, the eventual fate of a cell can be correlated with its birthdate. We describe how we used this method to study the development of trigeminal sensory neurons and discuss how the technique can be extended to the study of other organs.

Original languageEnglish (US)
Pages (from-to)87-92
Number of pages6
JournalCold Spring Harbor Protocols
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2012

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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