BAPTI and BAPTISM birthdating of neurons in zebrafish

Yu Chin Albert Pan, Sophie J C Caron, Alexander F. Schier

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This protocol describes how the photoconvertible protein Kaede can be used to determine the birthdates of neurons in live zebrafish. The methods used are birthdating analysis by photoconverted fluorescent protein tracing in vivo (BAPTI) and BAPTI combined with subpopulation markers (BAPTISM). Because Kaede can be converted from green to red fluorescence at any developmental time point, it serves as a temporal landmark for cell birth. When it is used in combination with subpopulation markers, the eventual fate of a cell can be correlated with its birthdate. We describe how we used this method to study the development of trigeminal sensory neurons and discuss how the technique can be extended to the study of other organs.

Original languageEnglish (US)
Pages (from-to)87-92
Number of pages6
JournalCold Spring Harbor Protocols
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2012

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Zebrafish
Neurons
Sensory Receptor Cells
Fluorescent Antibody Technique
Proteins
Fluorescence
Parturition

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

BAPTI and BAPTISM birthdating of neurons in zebrafish. / Pan, Yu Chin Albert; Caron, Sophie J C; Schier, Alexander F.

In: Cold Spring Harbor Protocols, Vol. 7, No. 1, 01.01.2012, p. 87-92.

Research output: Contribution to journalArticle

Pan, Yu Chin Albert ; Caron, Sophie J C ; Schier, Alexander F. / BAPTI and BAPTISM birthdating of neurons in zebrafish. In: Cold Spring Harbor Protocols. 2012 ; Vol. 7, No. 1. pp. 87-92.
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