BDNF-induced recruitment of TrkB receptor into neuronal lipid rafts

Roles in synaptic modulation

Shingo Suzuki, Tadahiro Numakawa, Kazuhiro Shimazu, Hisatsugu Koshimizu, Tomoko Hara, Hiroshi Hatanaka, Lin Mei, Bai Lu, Masami Kojima

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Brain-derived neurotrophic factor (BDNF) plays an important role in synoptic plasticity but the underlying signaling mechanisms remain unknown. Here, we show that BDNF rapidly recruits full-length TrkB (TrkB-FL) receptor into cholesterol-rich lipid rafts from nonraft regions of neuronal plasma membranes. Translocation of TrkB-FL was blocked by Trk inhibitors, suggesting a role of TrkB tyrosine kinase in the translocation. Disruption of lipid rafts by depleting cholesterol from cell surface blocked the ligand-induced translocation. Moreover, disruption of lipid rafts prevented potentiating effects of BDNF on transmitter release in cultured neurons and synoptic response to tetanus in hippocampal slices. In contrast, lipid rafts are not required for BDNF regulation of neuronal survival. Thus, ligand-induced TrkB translocation into lipid rafts may represent a signaling mechanism selective for synoptic modulation by BDNF in the central nervous system.

Original languageEnglish (US)
Pages (from-to)1205-1215
Number of pages11
JournalJournal of Cell Biology
Volume167
Issue number6
DOIs
StatePublished - Dec 20 2004

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trkB Receptor
Brain-Derived Neurotrophic Factor
Lipids
Cholesterol
Ligands
Tetanus
Protein-Tyrosine Kinases
Central Nervous System
Cell Membrane
Neurons

ASJC Scopus subject areas

  • Cell Biology

Cite this

Suzuki, S., Numakawa, T., Shimazu, K., Koshimizu, H., Hara, T., Hatanaka, H., ... Kojima, M. (2004). BDNF-induced recruitment of TrkB receptor into neuronal lipid rafts: Roles in synaptic modulation. Journal of Cell Biology, 167(6), 1205-1215. https://doi.org/10.1083/jcb.200404106

BDNF-induced recruitment of TrkB receptor into neuronal lipid rafts : Roles in synaptic modulation. / Suzuki, Shingo; Numakawa, Tadahiro; Shimazu, Kazuhiro; Koshimizu, Hisatsugu; Hara, Tomoko; Hatanaka, Hiroshi; Mei, Lin; Lu, Bai; Kojima, Masami.

In: Journal of Cell Biology, Vol. 167, No. 6, 20.12.2004, p. 1205-1215.

Research output: Contribution to journalArticle

Suzuki, S, Numakawa, T, Shimazu, K, Koshimizu, H, Hara, T, Hatanaka, H, Mei, L, Lu, B & Kojima, M 2004, 'BDNF-induced recruitment of TrkB receptor into neuronal lipid rafts: Roles in synaptic modulation', Journal of Cell Biology, vol. 167, no. 6, pp. 1205-1215. https://doi.org/10.1083/jcb.200404106
Suzuki S, Numakawa T, Shimazu K, Koshimizu H, Hara T, Hatanaka H et al. BDNF-induced recruitment of TrkB receptor into neuronal lipid rafts: Roles in synaptic modulation. Journal of Cell Biology. 2004 Dec 20;167(6):1205-1215. https://doi.org/10.1083/jcb.200404106
Suzuki, Shingo ; Numakawa, Tadahiro ; Shimazu, Kazuhiro ; Koshimizu, Hisatsugu ; Hara, Tomoko ; Hatanaka, Hiroshi ; Mei, Lin ; Lu, Bai ; Kojima, Masami. / BDNF-induced recruitment of TrkB receptor into neuronal lipid rafts : Roles in synaptic modulation. In: Journal of Cell Biology. 2004 ; Vol. 167, No. 6. pp. 1205-1215.
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