Behavioral and histologic neuroprotection of aqueous garlic extract after reversible focal cerebral ischemia

Sofiyan Saleem, Muzamil Ahmad, Abdullah Shafique Ahmad, Seema Yousuf, Mubeen Ahmad Ansari, Mohammad Badruzzaman Khan, Tauheed Ishrat, Fakhrul Islam

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The objective of the present study was to investigate the effects of aqueous garlic extract (AGE) on neurobehavioral activities, malondialdehyde (MDA) and reduced glutathione (GSH) levels, glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), and sodium-potassium ATPase (Na +,K+-ATPase) activities, and glutamate and aspartate content in a middle cerebral artery (MCA) occlusion (MCAO) model of acute cerebral ischemia in rats. The right MCA of male Wistar rats was occluded for 2 hours using intraluminal 4-0 monofilament, and reperfusion was allowed for 22 hours. MCAO caused significant depletion in GSH and its dependent enzymes (GPx, GR, and GST) and significant elevation of MDA, glutamate, and aspartate. The activities of Na+,K+-ATPase, SOD, and CAT were decreased significantly by MCAO. The neurobehavioral activities (grip strength, spontaneous motor activity, and motor coordination) were also decreased significantly in the MCAO group. All of the alterations induced by ischemia were significantly attenuated by pretreatment with AGE (500 mg/mL/kg of body weight, i.p.) 30 minutes before the induction of MCAO and correlated well with histopathology by decreasing the neuronal cell death following MCAO and reperfusion. These findings suggest that AGE effectively modulates neurobehavioral and neurochemical changes in focal ischemia, most probably by virtue of its antioxidant properties.

Original languageEnglish (US)
Pages (from-to)537-544
Number of pages8
JournalJournal of Medicinal Food
Volume9
Issue number4
DOIs
StatePublished - Jan 1 2006
Externally publishedYes

Fingerprint

Garlic
Brain Ischemia
Glutathione Reductase
Glutathione Peroxidase
Glutathione Transferase
Malondialdehyde
Aspartic Acid
Catalase
Superoxide Dismutase
Reperfusion
Glutamic Acid
Ischemia
Sodium-Potassium-Exchanging ATPase
Middle Cerebral Artery Infarction
Middle Cerebral Artery
Hand Strength
Glutathione
Wistar Rats
Motor Activity
Cell Death

Keywords

  • Cerebral ischemia
  • Garlic
  • Herbal drugs
  • Neurobehavior
  • Neuroprotection
  • Oxidative stress

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Behavioral and histologic neuroprotection of aqueous garlic extract after reversible focal cerebral ischemia. / Saleem, Sofiyan; Ahmad, Muzamil; Ahmad, Abdullah Shafique; Yousuf, Seema; Ansari, Mubeen Ahmad; Khan, Mohammad Badruzzaman; Ishrat, Tauheed; Islam, Fakhrul.

In: Journal of Medicinal Food, Vol. 9, No. 4, 01.01.2006, p. 537-544.

Research output: Contribution to journalArticle

Saleem, Sofiyan ; Ahmad, Muzamil ; Ahmad, Abdullah Shafique ; Yousuf, Seema ; Ansari, Mubeen Ahmad ; Khan, Mohammad Badruzzaman ; Ishrat, Tauheed ; Islam, Fakhrul. / Behavioral and histologic neuroprotection of aqueous garlic extract after reversible focal cerebral ischemia. In: Journal of Medicinal Food. 2006 ; Vol. 9, No. 4. pp. 537-544.
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