Bicarbonate promotes dye coupling in the epithelium and endothelium of the rabbit cornea

K. Keven Williams, Mitchell Aaron Watsky

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Purpose. Examine the mechanism of bicarbonate maintenance of cell-to-cell coupling in rabbit corneal epithelium and endothelium. Methods. Carboxyfluorescein was microinjected into rabbit corneal epithelial and endothelial cells. Adjacent cells were observed for fluorescence. Bathing solutions were buffered with bicarbonate, HEPES, phosphate, or acetate-citrate. The influence of intracellular pH and transmembrane voltage (Vm) were examined. Results. Bicarbonate was the only buffer to increase dye coupling. Substitution of bicarbonate structural analogs bisulfite and carbamate in a HEPES-buffered solution increased dye coupling in both cell types. Intracellular pH and Vm alterations in corneal epithelial cells bathed in HEPES vs. bicarbonate buffered media had no significant effects on dye coupling. Conclusions. Bicarbonate increases intercellular communication in the corneal epithelium and endothelium. This effect appears to result from an interaction of the bicarbonate molecule (or one of its structural analogs) with either gap junction proteins or an intermediary. We also demonstrate the presence of Cx43 in the rabbit corneal endothelium.

Original languageEnglish (US)
Pages (from-to)109-120
Number of pages12
JournalCurrent Eye Research
Volume28
Issue number2
DOIs
StatePublished - Feb 1 2004
Externally publishedYes

Fingerprint

Bicarbonates
Cornea
Endothelium
Coloring Agents
Epithelium
Rabbits
HEPES
Corneal Endothelium
Corneal Epithelium
Epithelial Cells
Connexin 43
Connexins
Carbamates
Citric Acid
Buffers
Acetates
Endothelial Cells
Fluorescence
Phosphates
Maintenance

Keywords

  • Corneal endothelium
  • Corneal epithelium
  • Cx43, HCO
  • Gap junction

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Bicarbonate promotes dye coupling in the epithelium and endothelium of the rabbit cornea. / Williams, K. Keven; Watsky, Mitchell Aaron.

In: Current Eye Research, Vol. 28, No. 2, 01.02.2004, p. 109-120.

Research output: Contribution to journalArticle

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AB - Purpose. Examine the mechanism of bicarbonate maintenance of cell-to-cell coupling in rabbit corneal epithelium and endothelium. Methods. Carboxyfluorescein was microinjected into rabbit corneal epithelial and endothelial cells. Adjacent cells were observed for fluorescence. Bathing solutions were buffered with bicarbonate, HEPES, phosphate, or acetate-citrate. The influence of intracellular pH and transmembrane voltage (Vm) were examined. Results. Bicarbonate was the only buffer to increase dye coupling. Substitution of bicarbonate structural analogs bisulfite and carbamate in a HEPES-buffered solution increased dye coupling in both cell types. Intracellular pH and Vm alterations in corneal epithelial cells bathed in HEPES vs. bicarbonate buffered media had no significant effects on dye coupling. Conclusions. Bicarbonate increases intercellular communication in the corneal epithelium and endothelium. This effect appears to result from an interaction of the bicarbonate molecule (or one of its structural analogs) with either gap junction proteins or an intermediary. We also demonstrate the presence of Cx43 in the rabbit corneal endothelium.

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