Bid activation in kidney cells following ATP depletion in vitro and ischemia in vivo

QingQing Wei, Mohammad M. Alam, Mong-Heng Wang, Fushin Yu, Zheng Dong

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Bid is a proapoptotic Bcl-2 family protein, which on activation translocates to mitochondria and induces damage to the organelles. Activation of Bid depends on its proteolytic processing into truncated forms of tBid. Bid is highly expressed in the kidneys; however, little is known about its role in renal pathophysiology. In this study, we initially examined Bid activation in cultured rat kidney proximal tubular cells following ATP depletion. The cells were depleted of ATP by azide incubation in the absence of metabolic substrates and then returned to normal culture medium for recovery. Typical apoptosis developed during recovery of ATP-depleted cells. This was accompanied by Bid cleavage, releasing tBid of 15 and 13 kDa. Bid cleavage was abolished in cells overexpressing Bcl-2, an antiapoptotic gene. It was also suppressed by caspase inhibitors. Peptide inhibitors of caspase-9 were more effective in blocking Bid cleavage compared with inhibitors of caspase-8 and caspase-3. Provision of glucose, a glycolytic substrate, during azide incubation inhibited Bid cleavage as well, indicating that Bid cleavage was initiated by ATP depletion. Consistently, Bid cleavage was also induced following ATP depletion by hypoxia or mitochondrial uncoupling. Of significance, cleaved Bid translocated to mitochondria, suggesting a role for Bid in the development of mitochondrial defects in ATP-depleted cells. Finally, Bid cleavage was induced during renal ischemia-reperfusion in the rat. Together, these results provide the first evidence for Bid activation in kidney cells following ATP depletion in vitro and renal ischemia in vivo.

Original languageEnglish (US)
Pages (from-to)F803-F809
JournalAmerican Journal of Physiology - Renal Physiology
Volume286
Issue number4 55-4
DOIs
StatePublished - Apr 2004

Keywords

  • Acute renal failure
  • Apoptosis
  • Caspase
  • Ischemia-reperfusion

ASJC Scopus subject areas

  • Physiology
  • Urology

Fingerprint Dive into the research topics of 'Bid activation in kidney cells following ATP depletion in vitro and ischemia in vivo'. Together they form a unique fingerprint.

  • Cite this