Bin1 mediates apoptosis by c-Myc in transformed primary cells

J. B. DuHadaway, Daitoku Sakamuro, D. L. Ewert, G. C. Prendergast

Research output: Contribution to journalArticle

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Abstract

The Bin1 gene encodes a c-Myc-interacting adapter protein with tumor suppressor and cell death properties. In this study, we offer evidence that Bin1 participates in a mechanism through which c-Myc activates programmed cell death in transformed primary chick or rat cells. Antisense or dominant inhibitory Bin1 genes did not affect the ability of c-Myc to drive proliferation or transformation, but they did reduce the susceptibility of cells to c-Myc-induced apoptosis. Protein-protein interaction was implicated, suggesting that Bin1 mediates a death or death sensitization signal from c-Myc. Our findings offer direct support for the "dual signal" model of Myc apoptotic function, based on interactions with a binding protein. Loss of Bin1 in human tumors may promote malignant progression in part by helping to stanch the death penalty associated with c-Myc activation.

Original languageEnglish (US)
Pages (from-to)3151-3156
Number of pages6
JournalCancer Research
Volume61
Issue number7
StatePublished - Apr 1 2001
Externally publishedYes

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Cell Death
Capital Punishment
Apoptosis
Tumor Suppressor Proteins
Genes
Carrier Proteins
Proteins
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

DuHadaway, J. B., Sakamuro, D., Ewert, D. L., & Prendergast, G. C. (2001). Bin1 mediates apoptosis by c-Myc in transformed primary cells. Cancer Research, 61(7), 3151-3156.

Bin1 mediates apoptosis by c-Myc in transformed primary cells. / DuHadaway, J. B.; Sakamuro, Daitoku; Ewert, D. L.; Prendergast, G. C.

In: Cancer Research, Vol. 61, No. 7, 01.04.2001, p. 3151-3156.

Research output: Contribution to journalArticle

DuHadaway, JB, Sakamuro, D, Ewert, DL & Prendergast, GC 2001, 'Bin1 mediates apoptosis by c-Myc in transformed primary cells', Cancer Research, vol. 61, no. 7, pp. 3151-3156.
DuHadaway JB, Sakamuro D, Ewert DL, Prendergast GC. Bin1 mediates apoptosis by c-Myc in transformed primary cells. Cancer Research. 2001 Apr 1;61(7):3151-3156.
DuHadaway, J. B. ; Sakamuro, Daitoku ; Ewert, D. L. ; Prendergast, G. C. / Bin1 mediates apoptosis by c-Myc in transformed primary cells. In: Cancer Research. 2001 ; Vol. 61, No. 7. pp. 3151-3156.
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