Bin1 mediates apoptosis by c-Myc in transformed primary cells

J. B. DuHadaway, D. Sakamuro, D. L. Ewert, G. C. Prendergast

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Abstract

The Bin1 gene encodes a c-Myc-interacting adapter protein with tumor suppressor and cell death properties. In this study, we offer evidence that Bin1 participates in a mechanism through which c-Myc activates programmed cell death in transformed primary chick or rat cells. Antisense or dominant inhibitory Bin1 genes did not affect the ability of c-Myc to drive proliferation or transformation, but they did reduce the susceptibility of cells to c-Myc-induced apoptosis. Protein-protein interaction was implicated, suggesting that Bin1 mediates a death or death sensitization signal from c-Myc. Our findings offer direct support for the "dual signal" model of Myc apoptotic function, based on interactions with a binding protein. Loss of Bin1 in human tumors may promote malignant progression in part by helping to stanch the death penalty associated with c-Myc activation.

Original languageEnglish (US)
Pages (from-to)3151-3156
Number of pages6
JournalCancer Research
Volume61
Issue number7
Publication statusPublished - Apr 1 2001
Externally publishedYes

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

DuHadaway, J. B., Sakamuro, D., Ewert, D. L., & Prendergast, G. C. (2001). Bin1 mediates apoptosis by c-Myc in transformed primary cells. Cancer Research, 61(7), 3151-3156.