Binding of a Staphylococcus aureus bone pathogen to type I collagen

Thomas B. Buxton, John Peter Rissing, Jack A. Horner, Kent Plowman, David F. Scott, Terry J. Sprinkle, Gary K. Best

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We contrasted the collagen-binding potential of the experimental osteomyelitis pathogen, Staphylococcus aureus strain SMH, to several other strains. These included Cowan 1 (binder), Wood 46 (non-binder) and six capsular variants. These measurements were made using an 125I-collagen binding assay. Formalin-killed S. aureus SMH strongly bound commercial type I iodinated collagen (dissociation contant, Kd = 2 × 10-9 m). The extent of binding was similar to Cowan 1. Binding was saturable and not inhibited by 100 mm solutions of d-glucose, d-galactose, d-mannose, methyl-α-l-fucopyranoside, l-hydroxyproline or l-glycine. d-lactose gave moderate inhibition of binding to collagen, and l-fucose was strongly inhibitory. Trypsinized SMH did not bind collagen. None of four Ruthenium-red-staining staphylococci (encapsulated) avidly bound type I collagen. The encapsulated Smith strain, for example, did not bind to collagen but its capsule-negative variant, Smith compact, showed extensive binding. Three of five non-encapsulated S. aureus were strong collagen binders. These data suggest that the prototype bone pathogen binds to the major protein component of bone's extracellular matrix. Collagen-binding is promoted by protein adhesin(s), not capsule. The latter, in fact, appeared to interfere with this interaction. Binding was inhibited by solutions containing the simple monosaccharide, l-fucose.

Original languageEnglish (US)
Pages (from-to)441-448
Number of pages8
JournalMicrobial Pathogenesis
Volume8
Issue number6
DOIs
StatePublished - Jun 1990

Keywords

  • Staphylococcus aureus
  • collagen
  • collagen-binding
  • l-fucose
  • osteomyelitis

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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    Buxton, T. B., Rissing, J. P., Horner, J. A., Plowman, K., Scott, D. F., Sprinkle, T. J., & Best, G. K. (1990). Binding of a Staphylococcus aureus bone pathogen to type I collagen. Microbial Pathogenesis, 8(6), 441-448. https://doi.org/10.1016/0882-4010(90)90031-K