Biochemical and Serological Characteristics of Natural 9-O-Acetyl GD3 from Human Melanoma and Bovine Buttermilk and Chemically O-Acetylated GD3

Gerd Ritter, Erika Boosfeld, Ellen Markstein, Herbert F. Oettgen, Lloyd J. Old, Philip O. Livingston, Robert K Yu, Shunlin Ren, William B. Stallcup

Research output: Contribution to journalArticle

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Abstract

Because its expression appears to be largely restricted to human melanomas, 9–0-acetyl-GD3 is a candidate antigen for vaccine construction. Searching for potential sources, we compared chemically 0-acety-lated calf brain GD3 and 9–0-acetyl-GD3 extracted from bovine buttermilk with 9–0-acetyl-GD3 from human melanoma. Three fractions (Fl-F3) of chemically 0-acetylated GD3 differed in the number and position of 0-acetyl groups. 0-Acetylation sites were the lactose portion in Fl and lactose as well as sialic acid in F2 and F3. Natural (melanoma- or buttermilk-derived) 9–0-acetyl-GD3 was O-acetylated solely on the sialic acid moiety. While Fl was not reactive with monoclonal antibodies against 9–0-acetyl-GD3, F2 and F3 were as reactive as the natural products. Immunization with the natural products induced high-titer antibodies against natural 9–0-acetyl-GD3 as well as F2 and F3. In contrast, mice immunized with the synthetic fractions produced antibodies only against the immunogen but not against natural 9–0-acetyl-GD3. Only immunization with the natural products induced production of antibodies reactive with surface antigens of melanoma cells expressing 9–0-acetyl-GD3. The findings suggest (a) that C-9 of the subterminal sialic acid is the site of chemical 0-acetylation in F2 and F3, as opposed to C-9 of the terminal sialic acid in the natural products; (b) that 0-acetylation of both the terminal and subterminal sialic acid moieties of GD3 results in recognition by three murine monoclonal antibodies (D1.1, ME 311, and Jones) reactive with human melanoma cells; (c) that 0-acetylation of the terminal sialic acid is critical, on the other hand, for inducing an immune response against melanoma 9–0-acetyl-GD3; and (</) that 0-acetyl GD3 from bovine buttermilk can substitute as immunogen for inducing an immune response against human melanoma cell surface antigens in the mouse.

Original languageEnglish (US)
Pages (from-to)1403-1410
Number of pages8
JournalCancer Research
Volume50
Issue number5
StatePublished - Mar 1 1990
Externally publishedYes

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N-Acetylneuraminic Acid
Melanoma
Acetylation
Biological Products
Lactose
Surface Antigens
Immunization
Monoclonal Antibodies
Antibodies
Buttermilk
Antibody Formation
Vaccines
Antigens
Brain

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Ritter, G., Boosfeld, E., Markstein, E., Oettgen, H. F., Old, L. J., Livingston, P. O., ... Stallcup, W. B. (1990). Biochemical and Serological Characteristics of Natural 9-O-Acetyl GD3 from Human Melanoma and Bovine Buttermilk and Chemically O-Acetylated GD3. Cancer Research, 50(5), 1403-1410.

Biochemical and Serological Characteristics of Natural 9-O-Acetyl GD3 from Human Melanoma and Bovine Buttermilk and Chemically O-Acetylated GD3. / Ritter, Gerd; Boosfeld, Erika; Markstein, Ellen; Oettgen, Herbert F.; Old, Lloyd J.; Livingston, Philip O.; Yu, Robert K; Ren, Shunlin; Stallcup, William B.

In: Cancer Research, Vol. 50, No. 5, 01.03.1990, p. 1403-1410.

Research output: Contribution to journalArticle

Ritter, G, Boosfeld, E, Markstein, E, Oettgen, HF, Old, LJ, Livingston, PO, Yu, RK, Ren, S & Stallcup, WB 1990, 'Biochemical and Serological Characteristics of Natural 9-O-Acetyl GD3 from Human Melanoma and Bovine Buttermilk and Chemically O-Acetylated GD3', Cancer Research, vol. 50, no. 5, pp. 1403-1410.
Ritter G, Boosfeld E, Markstein E, Oettgen HF, Old LJ, Livingston PO et al. Biochemical and Serological Characteristics of Natural 9-O-Acetyl GD3 from Human Melanoma and Bovine Buttermilk and Chemically O-Acetylated GD3. Cancer Research. 1990 Mar 1;50(5):1403-1410.
Ritter, Gerd ; Boosfeld, Erika ; Markstein, Ellen ; Oettgen, Herbert F. ; Old, Lloyd J. ; Livingston, Philip O. ; Yu, Robert K ; Ren, Shunlin ; Stallcup, William B. / Biochemical and Serological Characteristics of Natural 9-O-Acetyl GD3 from Human Melanoma and Bovine Buttermilk and Chemically O-Acetylated GD3. In: Cancer Research. 1990 ; Vol. 50, No. 5. pp. 1403-1410.
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abstract = "Because its expression appears to be largely restricted to human melanomas, 9–0-acetyl-GD3 is a candidate antigen for vaccine construction. Searching for potential sources, we compared chemically 0-acety-lated calf brain GD3 and 9–0-acetyl-GD3 extracted from bovine buttermilk with 9–0-acetyl-GD3 from human melanoma. Three fractions (Fl-F3) of chemically 0-acetylated GD3 differed in the number and position of 0-acetyl groups. 0-Acetylation sites were the lactose portion in Fl and lactose as well as sialic acid in F2 and F3. Natural (melanoma- or buttermilk-derived) 9–0-acetyl-GD3 was O-acetylated solely on the sialic acid moiety. While Fl was not reactive with monoclonal antibodies against 9–0-acetyl-GD3, F2 and F3 were as reactive as the natural products. Immunization with the natural products induced high-titer antibodies against natural 9–0-acetyl-GD3 as well as F2 and F3. In contrast, mice immunized with the synthetic fractions produced antibodies only against the immunogen but not against natural 9–0-acetyl-GD3. Only immunization with the natural products induced production of antibodies reactive with surface antigens of melanoma cells expressing 9–0-acetyl-GD3. The findings suggest (a) that C-9 of the subterminal sialic acid is the site of chemical 0-acetylation in F2 and F3, as opposed to C-9 of the terminal sialic acid in the natural products; (b) that 0-acetylation of both the terminal and subterminal sialic acid moieties of GD3 results in recognition by three murine monoclonal antibodies (D1.1, ME 311, and Jones) reactive with human melanoma cells; (c) that 0-acetylation of the terminal sialic acid is critical, on the other hand, for inducing an immune response against melanoma 9–0-acetyl-GD3; and (",
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