Biochemical characterization of autologous fibrinogen adhesive

Robert A. Weisman, Arthur J Torsiglieri, Alan D. Schreiber, Gordon H. Epstein

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Fibrinogen-based adhesive, derived from pooled human plasma, has been used in Europe with great success in otologic surgery, but has not been approved for use in the U.S. because of the risk of transmitting hepatitis. Autologous fibrinogen, derived by polyethylene glycol precipitation from the blood of an individual patient would avoid this risk, and has been shown to be relatively safe to the ear in animal studies. A study of the biochemical composition of this autologous fibrinogen concentrate derived from 15 human volunteer donors was performed. The mean starting plasma fibrinogen was 2.12 mg/ml (range 1.59–3.22 mg/ml). When 10% polyethylene glycol was used to precipitate the fibrinogen, the concentrate contained, on the average, 31.8 mg of fibrinogen/ml. The percent yield averaged 54.9%, and the protein in the final product was 91.9% fibrinogen. Increasing the polyethylene glycol concentration in the precipitation process to as high as 15% resulted in an increased yield as high as 91%, but the protein in the final product was only 42.5% fibrinogen. Polyacrylamide gel electrophoresis confirmed that the predominant protein in the 10% polyethylene glycol precipitate was fibrinogen. These data suggest that highly concentrated fibrinogen can be derived with relative ease from single donor human plasma, and that the product is relatively pure. When combined with thrombin and calcium chloride, this concentrate should provide an adhesive that avoids the risks associated with fibrinogen adhesive derived from pooled blood.

Original languageEnglish (US)
Pages (from-to)1186-1190
Number of pages5
JournalLaryngoscope
Volume97
Issue number10
StatePublished - Jan 1 1987

Fingerprint

Fibrin Tissue Adhesive
Fibrinogen
Tissue Donors
Calcium Chloride
Proteins
Thrombin
Adhesives
Hepatitis
Ear
Polyacrylamide Gel Electrophoresis
Volunteers

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Weisman, R. A., Torsiglieri, A. J., Schreiber, A. D., & Epstein, G. H. (1987). Biochemical characterization of autologous fibrinogen adhesive. Laryngoscope, 97(10), 1186-1190.

Biochemical characterization of autologous fibrinogen adhesive. / Weisman, Robert A.; Torsiglieri, Arthur J; Schreiber, Alan D.; Epstein, Gordon H.

In: Laryngoscope, Vol. 97, No. 10, 01.01.1987, p. 1186-1190.

Research output: Contribution to journalArticle

Weisman, RA, Torsiglieri, AJ, Schreiber, AD & Epstein, GH 1987, 'Biochemical characterization of autologous fibrinogen adhesive', Laryngoscope, vol. 97, no. 10, pp. 1186-1190.
Weisman RA, Torsiglieri AJ, Schreiber AD, Epstein GH. Biochemical characterization of autologous fibrinogen adhesive. Laryngoscope. 1987 Jan 1;97(10):1186-1190.
Weisman, Robert A. ; Torsiglieri, Arthur J ; Schreiber, Alan D. ; Epstein, Gordon H. / Biochemical characterization of autologous fibrinogen adhesive. In: Laryngoscope. 1987 ; Vol. 97, No. 10. pp. 1186-1190.
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