Abstract
This study compares the molecular, charge and lectin microheterogeneity of bovine alpha-fetoprotein (bAFP) with human (h) AFP. Molecular weights of bAFP (81 kDa) and hAFP (69 kDa) were detected by Western immunoblotting. Marked crossreactivity was found between bAFP and hAFP by Western immunoblotting but no crossreactivity was noticed by radioimmunoassays. At least seven charge isoforms of bAFP and three isoforms of hAFP were consistently detected by chromatofocusing in a mixture of fetal bovine serum (FBS) and human cord blood (hCB), while only three isoforms of bAFP and hAFP were detected in a mixture of bovine (bAF) and human amniotic fluid (hAF). Using concanavalin A (Con A) chromatography, 50% of bAFP was Con A reactive and 50% non-reactive, while more than 98% of hAFP was Con A reactive in a mixture of FBS and hCB. However, in AFs, 72% of bAFP was Con A reactive, while 89% of hAFP was Con A reactive. These data indicate that marked differences exist in both the charge and lectin microheterogeneity of bovine and human AFP.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 327-336 |
| Number of pages | 10 |
| Journal | Comparative Biochemistry and Physiology -- Part B: Biochemistry and |
| Volume | 108 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jul 1994 |
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Keywords
- Amniotic fluid
- Bovine alpha-fetoprotein
- Human alpha-fetoprotein
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Molecular Biology
Cite this
Biochemical characterization of bovine alpha-fetoprotein and comparison with human alpha-fetoprotein. / He, Yongsheng; Keel, Brooks A.
In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 108, No. 3, 07.1994, p. 327-336.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biochemical characterization of bovine alpha-fetoprotein and comparison with human alpha-fetoprotein
AU - He, Yongsheng
AU - Keel, Brooks A.
PY - 1994/7
Y1 - 1994/7
N2 - This study compares the molecular, charge and lectin microheterogeneity of bovine alpha-fetoprotein (bAFP) with human (h) AFP. Molecular weights of bAFP (81 kDa) and hAFP (69 kDa) were detected by Western immunoblotting. Marked crossreactivity was found between bAFP and hAFP by Western immunoblotting but no crossreactivity was noticed by radioimmunoassays. At least seven charge isoforms of bAFP and three isoforms of hAFP were consistently detected by chromatofocusing in a mixture of fetal bovine serum (FBS) and human cord blood (hCB), while only three isoforms of bAFP and hAFP were detected in a mixture of bovine (bAF) and human amniotic fluid (hAF). Using concanavalin A (Con A) chromatography, 50% of bAFP was Con A reactive and 50% non-reactive, while more than 98% of hAFP was Con A reactive in a mixture of FBS and hCB. However, in AFs, 72% of bAFP was Con A reactive, while 89% of hAFP was Con A reactive. These data indicate that marked differences exist in both the charge and lectin microheterogeneity of bovine and human AFP.
AB - This study compares the molecular, charge and lectin microheterogeneity of bovine alpha-fetoprotein (bAFP) with human (h) AFP. Molecular weights of bAFP (81 kDa) and hAFP (69 kDa) were detected by Western immunoblotting. Marked crossreactivity was found between bAFP and hAFP by Western immunoblotting but no crossreactivity was noticed by radioimmunoassays. At least seven charge isoforms of bAFP and three isoforms of hAFP were consistently detected by chromatofocusing in a mixture of fetal bovine serum (FBS) and human cord blood (hCB), while only three isoforms of bAFP and hAFP were detected in a mixture of bovine (bAF) and human amniotic fluid (hAF). Using concanavalin A (Con A) chromatography, 50% of bAFP was Con A reactive and 50% non-reactive, while more than 98% of hAFP was Con A reactive in a mixture of FBS and hCB. However, in AFs, 72% of bAFP was Con A reactive, while 89% of hAFP was Con A reactive. These data indicate that marked differences exist in both the charge and lectin microheterogeneity of bovine and human AFP.
KW - Amniotic fluid
KW - Bovine alpha-fetoprotein
KW - Human alpha-fetoprotein
UR - http://www.scopus.com/inward/record.url?scp=0028124236&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028124236&partnerID=8YFLogxK
U2 - 10.1016/0305-0491(94)90084-1
DO - 10.1016/0305-0491(94)90084-1
M3 - Article
C2 - 7521727
AN - SCOPUS:0028124236
VL - 108
SP - 327
EP - 336
JO - Comparative Biochemistry and Physiology -- Part B: Biochemistry and
JF - Comparative Biochemistry and Physiology -- Part B: Biochemistry and
SN - 0305-0491
IS - 3
ER -