Biochemical characterization of bovine alpha-fetoprotein and comparison with human alpha-fetoprotein

Yongsheng He, Brooks Allen Keel

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This study compares the molecular, charge and lectin microheterogeneity of bovine alpha-fetoprotein (bAFP) with human (h) AFP. Molecular weights of bAFP (81 kDa) and hAFP (69 kDa) were detected by Western immunoblotting. Marked crossreactivity was found between bAFP and hAFP by Western immunoblotting but no crossreactivity was noticed by radioimmunoassays. At least seven charge isoforms of bAFP and three isoforms of hAFP were consistently detected by chromatofocusing in a mixture of fetal bovine serum (FBS) and human cord blood (hCB), while only three isoforms of bAFP and hAFP were detected in a mixture of bovine (bAF) and human amniotic fluid (hAF). Using concanavalin A (Con A) chromatography, 50% of bAFP was Con A reactive and 50% non-reactive, while more than 98% of hAFP was Con A reactive in a mixture of FBS and hCB. However, in AFs, 72% of bAFP was Con A reactive, while 89% of hAFP was Con A reactive. These data indicate that marked differences exist in both the charge and lectin microheterogeneity of bovine and human AFP.

Original languageEnglish (US)
Pages (from-to)327-336
Number of pages10
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume108
Issue number3
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Fingerprint

alpha-Fetoproteins
Concanavalin A
Protein Isoforms
Lectins
Blood
Fetal Blood
Chromatography
human AFP protein
Western Blotting
Molecular weight
Amniotic Fluid
Serum
Fluids
Radioimmunoassay
Molecular Weight

Keywords

  • Amniotic fluid
  • Bovine alpha-fetoprotein
  • Human alpha-fetoprotein

ASJC Scopus subject areas

  • Biochemistry
  • Physiology

Cite this

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abstract = "This study compares the molecular, charge and lectin microheterogeneity of bovine alpha-fetoprotein (bAFP) with human (h) AFP. Molecular weights of bAFP (81 kDa) and hAFP (69 kDa) were detected by Western immunoblotting. Marked crossreactivity was found between bAFP and hAFP by Western immunoblotting but no crossreactivity was noticed by radioimmunoassays. At least seven charge isoforms of bAFP and three isoforms of hAFP were consistently detected by chromatofocusing in a mixture of fetal bovine serum (FBS) and human cord blood (hCB), while only three isoforms of bAFP and hAFP were detected in a mixture of bovine (bAF) and human amniotic fluid (hAF). Using concanavalin A (Con A) chromatography, 50{\%} of bAFP was Con A reactive and 50{\%} non-reactive, while more than 98{\%} of hAFP was Con A reactive in a mixture of FBS and hCB. However, in AFs, 72{\%} of bAFP was Con A reactive, while 89{\%} of hAFP was Con A reactive. These data indicate that marked differences exist in both the charge and lectin microheterogeneity of bovine and human AFP.",
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AB - This study compares the molecular, charge and lectin microheterogeneity of bovine alpha-fetoprotein (bAFP) with human (h) AFP. Molecular weights of bAFP (81 kDa) and hAFP (69 kDa) were detected by Western immunoblotting. Marked crossreactivity was found between bAFP and hAFP by Western immunoblotting but no crossreactivity was noticed by radioimmunoassays. At least seven charge isoforms of bAFP and three isoforms of hAFP were consistently detected by chromatofocusing in a mixture of fetal bovine serum (FBS) and human cord blood (hCB), while only three isoforms of bAFP and hAFP were detected in a mixture of bovine (bAF) and human amniotic fluid (hAF). Using concanavalin A (Con A) chromatography, 50% of bAFP was Con A reactive and 50% non-reactive, while more than 98% of hAFP was Con A reactive in a mixture of FBS and hCB. However, in AFs, 72% of bAFP was Con A reactive, while 89% of hAFP was Con A reactive. These data indicate that marked differences exist in both the charge and lectin microheterogeneity of bovine and human AFP.

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