Biochemical Study of Heterosis for Brain Myelin Content in Mice

H. Ebato, T. N. Seyfried, Robert K Yu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Abstract: Heterosis (hybrid vigor) for brain myelin content has been examined in detail in (C57BL/6J × DBA/ 2J)F1 hybrid mice at 17 days of age. The amount of myelin isolated from the F1 hybrid brain is greater than that isolated from either parental strain. In addition, the total protein content in the myelin of the three genotypes showed the following trend: F1 > DBA > C57. However, no discernible differences in myelin protein compositions could be detected by sodium dodecyl sulfate‐poly‐acrylamide gel electrophoresis. Analysis of the whole brain for several myelin‐associated constituents such as GM1 ganglioside, 2′, 3′‐cyclic nucleotide 3′‐phosphohy‐drolase (CNPase), 5′‐nucleotidase and carbonic anhy‐drase indicated that heterosis exists for these components. No heterosis was found for such nonmyelin constituents as gangliosides GDIa, GT, GQ, RNA, DNA and choline acetyltransferase. A developmental study of the whole brain CNPase indicated that the heterotic effect was greatest during the most active period of myelination (17–30 days). We conclude that the heterotic effect is specific for myelin content and is probably the result of an accelerated myelin synthesis. The heterotic effect should have great potential as a new model for studying aspects of myelinogenesis.

Original languageEnglish (US)
Pages (from-to)440-446
Number of pages7
JournalJournal of Neurochemistry
Volume40
Issue number2
DOIs
StatePublished - Jan 1 1983
Externally publishedYes

Fingerprint

Hybrid Vigor
Myelin Sheath
2',3'-Cyclic-Nucleotide Phosphodiesterases
Brain
G(M1) Ganglioside
Myelin Proteins
Carbonic Anhydrases
Choline O-Acetyltransferase
Gangliosides
Electrophoresis
Nucleotides
Gels
Sodium
RNA
Genotype
DNA
Chemical analysis
Proteins

Keywords

  • 2′‐3′‐Cyclic nucleotide 3′‐phosphohydro‐lase
  • 5′‐Nucleotidase
  • Carbonic anhydrase
  • Ganglioside
  • Heterosis
  • Myelin

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Biochemical Study of Heterosis for Brain Myelin Content in Mice. / Ebato, H.; Seyfried, T. N.; Yu, Robert K.

In: Journal of Neurochemistry, Vol. 40, No. 2, 01.01.1983, p. 440-446.

Research output: Contribution to journalArticle

Ebato, H. ; Seyfried, T. N. ; Yu, Robert K. / Biochemical Study of Heterosis for Brain Myelin Content in Mice. In: Journal of Neurochemistry. 1983 ; Vol. 40, No. 2. pp. 440-446.
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