Biochemical study of individual zonae from human oocytes that failed to undergo fertilization in intracytoplasmic sperm injection

Vera Gross, Anil Dubey, Alan S. Penzias, Lawrence C Layman, Richard Reindollar, Tom Ducibella

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Successful intracytoplasmic sperm injection (ICSI) is dependent upon the competence of the oocyte to respond to the injection of a spermatozoon in the presence of calcium. This study determined if oocytes that failed to become fertilized (absence of any pronuclei) failed to undergo cytoplasmic activation, as ascertained by an electrophoretic shift in the zona pellucida (zona) protein, huZP2. Of 48 zonae individually analysed, 58% did not have a detectable huZP2 shift. Three patterns were observed. In seven patients, none of the unfertilized oocyte huZP2 shifted (16 out of 48); in two, all zonae exhibited a huZP2 shift (five out of 48); in eight, there was a mixture of shifted and unshifted (27 out of 48) in each case. There was no clear relationship between the presence of the huZP2 shift and maternal age, pregnancy outcome, male-factor infertility, or fertilization rate. However, in six couples diagnosed as having no detectable male-factor infertility, 73% (11 out of 15) of the zonae had unshifted huZP2, suggesting that an oocyte defect is involved in some cases of failed fertilization after ICSI. One likely cause for the absence of the huZP2 shift is a failure of cytoplasmic activation. Since oocytes were injected at metaphase II, we hypothesize that some oocytes that failed to fertilize have completed nuclear (meiotic) without cytoplasmic maturation.

Original languageEnglish (US)
Pages (from-to)959-965
Number of pages7
JournalMolecular Human Reproduction
Volume2
Issue number12
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Fingerprint

Intracytoplasmic Sperm Injections
Herpes Zoster
Fertilization
Oocytes
Male Infertility
Zona Pellucida
Maternal Age
Pregnancy Outcome
Metaphase
Mental Competency
Spermatozoa
Calcium
Injections
Proteins

Keywords

  • Calcium
  • Human
  • Oocytes
  • Spermatozoa
  • Zona pellucida

ASJC Scopus subject areas

  • Reproductive Medicine
  • Embryology
  • Molecular Biology
  • Genetics
  • Obstetrics and Gynecology
  • Developmental Biology
  • Cell Biology

Cite this

Biochemical study of individual zonae from human oocytes that failed to undergo fertilization in intracytoplasmic sperm injection. / Gross, Vera; Dubey, Anil; Penzias, Alan S.; Layman, Lawrence C; Reindollar, Richard; Ducibella, Tom.

In: Molecular Human Reproduction, Vol. 2, No. 12, 01.01.1996, p. 959-965.

Research output: Contribution to journalArticle

Gross, Vera ; Dubey, Anil ; Penzias, Alan S. ; Layman, Lawrence C ; Reindollar, Richard ; Ducibella, Tom. / Biochemical study of individual zonae from human oocytes that failed to undergo fertilization in intracytoplasmic sperm injection. In: Molecular Human Reproduction. 1996 ; Vol. 2, No. 12. pp. 959-965.
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