Biodegradable mesoporous delivery system for biomineralization precursors

Hong Ye Yang, Li Na Niu, Jin Long Sun, Xue Qing Huang, Dan Dan Pei, Cui Huang, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Scaffold supplements such as nanoparticles, components of the extracellular matrix, or growth factors have been incorporated in conventional scaffold materials to produce smart scaffolds for tissue engineering of damaged hard tissues. Due to increasing concerns on the clinical side effects of using large doses of recombinant bone-morphogenetic protein-2 in bone surgery, it is desirable to develop an alternative nanoscale scaffold supplement that is not only osteoinductive, but is also multifunctional in that it can perform other significant bone regenerative roles apart from stimulation of osteogenic differentiation. Because both amorphous calcium phosphate (ACP) and silica are osteoinductive, a biodegradable, nonfunctionalized, expanded-pore mesoporous silica nanoparticle carrier was developed for loading, storage, and sustained release of a novel, biosilicification-inspired, polyamine-stabilized liquid precursor phase of ACP for collagen biomineralization and for release of orthosilicic acid, both of which are conducive to bone growth. Positively charged poly(allylamine)-stabilized ACP (PAH-ACP) could be effectively loaded and released from nonfunctionalized expanded-pore mesoporous silica nanoparticles (pMSN). The PAH-ACP released from loaded pMSN still retained its ability to infiltrate and mineralize collagen fibrils. Complete degradation of pMSN occurred following unloading of their PAH-ACP cargo. Because PAH-ACP loaded pMSN possesses relatively low cytotoxicity to human bone marrow-derived mesenchymal stem cells, these nanoparticles may be blended with any osteoconductive scaffold with macro- and microporosities as a versatile scaffold supplement to enhance bone regeneration.

Original languageEnglish (US)
Pages (from-to)839-854
Number of pages16
JournalInternational journal of nanomedicine
Volume12
DOIs
StatePublished - Jan 25 2017

Fingerprint

Biomineralization
Calcium phosphate
Nanoparticles
Silicon Dioxide
Bone
Silica
Polycyclic aromatic hydrocarbons
Scaffolds (biology)
Scaffolds
Collagen
Allylamine
Bone and Bones
Bone Morphogenetic Protein 2
Microporosity
Bone Regeneration
Bone Development
Polyamines
Tissue Engineering
Cytotoxicity
amorphous calcium phosphate

Keywords

  • Amorphous calcium phosphate
  • Biomineralization
  • Collagen
  • Mesoporous silica
  • Osteoinductive
  • Poly(allylamine)

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

Cite this

Biodegradable mesoporous delivery system for biomineralization precursors. / Yang, Hong Ye; Niu, Li Na; Sun, Jin Long; Huang, Xue Qing; Pei, Dan Dan; Huang, Cui; Tay, Franklin Chi Meng.

In: International journal of nanomedicine, Vol. 12, 25.01.2017, p. 839-854.

Research output: Contribution to journalArticle

Yang, Hong Ye ; Niu, Li Na ; Sun, Jin Long ; Huang, Xue Qing ; Pei, Dan Dan ; Huang, Cui ; Tay, Franklin Chi Meng. / Biodegradable mesoporous delivery system for biomineralization precursors. In: International journal of nanomedicine. 2017 ; Vol. 12. pp. 839-854.
@article{df1eb985282d42c6bd1b6a3bd43a767b,
title = "Biodegradable mesoporous delivery system for biomineralization precursors",
abstract = "Scaffold supplements such as nanoparticles, components of the extracellular matrix, or growth factors have been incorporated in conventional scaffold materials to produce smart scaffolds for tissue engineering of damaged hard tissues. Due to increasing concerns on the clinical side effects of using large doses of recombinant bone-morphogenetic protein-2 in bone surgery, it is desirable to develop an alternative nanoscale scaffold supplement that is not only osteoinductive, but is also multifunctional in that it can perform other significant bone regenerative roles apart from stimulation of osteogenic differentiation. Because both amorphous calcium phosphate (ACP) and silica are osteoinductive, a biodegradable, nonfunctionalized, expanded-pore mesoporous silica nanoparticle carrier was developed for loading, storage, and sustained release of a novel, biosilicification-inspired, polyamine-stabilized liquid precursor phase of ACP for collagen biomineralization and for release of orthosilicic acid, both of which are conducive to bone growth. Positively charged poly(allylamine)-stabilized ACP (PAH-ACP) could be effectively loaded and released from nonfunctionalized expanded-pore mesoporous silica nanoparticles (pMSN). The PAH-ACP released from loaded pMSN still retained its ability to infiltrate and mineralize collagen fibrils. Complete degradation of pMSN occurred following unloading of their PAH-ACP cargo. Because PAH-ACP loaded pMSN possesses relatively low cytotoxicity to human bone marrow-derived mesenchymal stem cells, these nanoparticles may be blended with any osteoconductive scaffold with macro- and microporosities as a versatile scaffold supplement to enhance bone regeneration.",
keywords = "Amorphous calcium phosphate, Biomineralization, Collagen, Mesoporous silica, Osteoinductive, Poly(allylamine)",
author = "Yang, {Hong Ye} and Niu, {Li Na} and Sun, {Jin Long} and Huang, {Xue Qing} and Pei, {Dan Dan} and Cui Huang and Tay, {Franklin Chi Meng}",
year = "2017",
month = "1",
day = "25",
doi = "10.2147/IJN.S128792",
language = "English (US)",
volume = "12",
pages = "839--854",
journal = "International Journal of Nanomedicine",
issn = "1176-9114",
publisher = "Dove Medical Press Ltd.",

}

TY - JOUR

T1 - Biodegradable mesoporous delivery system for biomineralization precursors

AU - Yang, Hong Ye

AU - Niu, Li Na

AU - Sun, Jin Long

AU - Huang, Xue Qing

AU - Pei, Dan Dan

AU - Huang, Cui

AU - Tay, Franklin Chi Meng

PY - 2017/1/25

Y1 - 2017/1/25

N2 - Scaffold supplements such as nanoparticles, components of the extracellular matrix, or growth factors have been incorporated in conventional scaffold materials to produce smart scaffolds for tissue engineering of damaged hard tissues. Due to increasing concerns on the clinical side effects of using large doses of recombinant bone-morphogenetic protein-2 in bone surgery, it is desirable to develop an alternative nanoscale scaffold supplement that is not only osteoinductive, but is also multifunctional in that it can perform other significant bone regenerative roles apart from stimulation of osteogenic differentiation. Because both amorphous calcium phosphate (ACP) and silica are osteoinductive, a biodegradable, nonfunctionalized, expanded-pore mesoporous silica nanoparticle carrier was developed for loading, storage, and sustained release of a novel, biosilicification-inspired, polyamine-stabilized liquid precursor phase of ACP for collagen biomineralization and for release of orthosilicic acid, both of which are conducive to bone growth. Positively charged poly(allylamine)-stabilized ACP (PAH-ACP) could be effectively loaded and released from nonfunctionalized expanded-pore mesoporous silica nanoparticles (pMSN). The PAH-ACP released from loaded pMSN still retained its ability to infiltrate and mineralize collagen fibrils. Complete degradation of pMSN occurred following unloading of their PAH-ACP cargo. Because PAH-ACP loaded pMSN possesses relatively low cytotoxicity to human bone marrow-derived mesenchymal stem cells, these nanoparticles may be blended with any osteoconductive scaffold with macro- and microporosities as a versatile scaffold supplement to enhance bone regeneration.

AB - Scaffold supplements such as nanoparticles, components of the extracellular matrix, or growth factors have been incorporated in conventional scaffold materials to produce smart scaffolds for tissue engineering of damaged hard tissues. Due to increasing concerns on the clinical side effects of using large doses of recombinant bone-morphogenetic protein-2 in bone surgery, it is desirable to develop an alternative nanoscale scaffold supplement that is not only osteoinductive, but is also multifunctional in that it can perform other significant bone regenerative roles apart from stimulation of osteogenic differentiation. Because both amorphous calcium phosphate (ACP) and silica are osteoinductive, a biodegradable, nonfunctionalized, expanded-pore mesoporous silica nanoparticle carrier was developed for loading, storage, and sustained release of a novel, biosilicification-inspired, polyamine-stabilized liquid precursor phase of ACP for collagen biomineralization and for release of orthosilicic acid, both of which are conducive to bone growth. Positively charged poly(allylamine)-stabilized ACP (PAH-ACP) could be effectively loaded and released from nonfunctionalized expanded-pore mesoporous silica nanoparticles (pMSN). The PAH-ACP released from loaded pMSN still retained its ability to infiltrate and mineralize collagen fibrils. Complete degradation of pMSN occurred following unloading of their PAH-ACP cargo. Because PAH-ACP loaded pMSN possesses relatively low cytotoxicity to human bone marrow-derived mesenchymal stem cells, these nanoparticles may be blended with any osteoconductive scaffold with macro- and microporosities as a versatile scaffold supplement to enhance bone regeneration.

KW - Amorphous calcium phosphate

KW - Biomineralization

KW - Collagen

KW - Mesoporous silica

KW - Osteoinductive

KW - Poly(allylamine)

UR - http://www.scopus.com/inward/record.url?scp=85011537371&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85011537371&partnerID=8YFLogxK

U2 - 10.2147/IJN.S128792

DO - 10.2147/IJN.S128792

M3 - Article

C2 - 28182119

AN - SCOPUS:85011537371

VL - 12

SP - 839

EP - 854

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

SN - 1176-9114

ER -