Biodegradable polymer scaffolds loaded with low-dose BMP-2 stimulate periodontal ligament cell differentiation

Anders Skodje, Shaza Bushra M Idris, Yang Sun, Sushma Bartaula, Kamal Mustafa, Anna Finne-Wistrand, Ulf M E Wikesjö, Knut N. Leknes

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Poly(l-lactide)-co-(epsilon-caprolactone) [poly(LLA-co-CL)] and poly(l-lactide)-co-(1,5-dioxepan-2-one) [poly(LLA-co-DXO)] are being considered candidate scaffolds for bone tissue engineering. We evaluated the bioactive potential of poly(LLA-co-CL) and poly(LLA-co-DXO) scaffolds loaded with low-dose bone morphogenetic protein-2 (BMP-2). Periodontal ligament (PDL) cells were cultured onto the various scaffolds loaded with 1 μg BMP-2 or without BMP-2 (control). Cell viability, attachment, and proliferation were determined using a methylthiazol tetrazolium (MTT) colorimetric assay at day 1, 3, and 7. Scanning electron microscopy was used to analyze cell morphology at day 7. Cell differentiation was evaluated assaying alkaline phosphatase (ALP) activity at day 7, 14, and 21. Real-time PCR was used to evaluate the mRNA expression of periostin, ALP, type I collagen, bone sialoprotein and BMP-2. A commercially available enzyme-linked immunosorbent assay was used to assess BMP-2 production. Surface analysis disclosed excellent cell attachment, spread, and penetration into the porous scaffolds. The MTT assay indicated that scaffolds loaded with low concentration of BMP-2 did not influence the viability of cells. Cells grown on the modified scaffolds expressed higher levels of osteogenic markers than the nonmodified scaffolds (p<0.05). Poly(LLA-co-CL) and poly(LLA-co-DXO) scaffolds loaded with low-dose BMP-2 exhibited a significant effect stimulating PDL differentiation suggesting a continued evaluation in relevant in vivo models.

Original languageEnglish (US)
Pages (from-to)1991-1998
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume103
Issue number6
DOIs
Publication statusPublished - Jun 1 2015

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Keywords

  • BMP-2
  • bioengineering
  • cell biology
  • differentiation
  • polymer scaffolds

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Skodje, A., Idris, S. B. M., Sun, Y., Bartaula, S., Mustafa, K., Finne-Wistrand, A., ... Leknes, K. N. (2015). Biodegradable polymer scaffolds loaded with low-dose BMP-2 stimulate periodontal ligament cell differentiation. Journal of Biomedical Materials Research - Part A, 103(6), 1991-1998. https://doi.org/10.1002/jbm.a.35334