Biodegradable polymer scaffolds loaded with low-dose BMP-2 stimulate periodontal ligament cell differentiation

Anders Skodje, Shaza Bushra M Idris, Yang Sun, Sushma Bartaula, Kamal Mustafa, Anna Finne-Wistrand, Ulf M E Wikesjö, Knut N. Leknes

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Poly(l-lactide)-co-(epsilon-caprolactone) [poly(LLA-co-CL)] and poly(l-lactide)-co-(1,5-dioxepan-2-one) [poly(LLA-co-DXO)] are being considered candidate scaffolds for bone tissue engineering. We evaluated the bioactive potential of poly(LLA-co-CL) and poly(LLA-co-DXO) scaffolds loaded with low-dose bone morphogenetic protein-2 (BMP-2). Periodontal ligament (PDL) cells were cultured onto the various scaffolds loaded with 1 μg BMP-2 or without BMP-2 (control). Cell viability, attachment, and proliferation were determined using a methylthiazol tetrazolium (MTT) colorimetric assay at day 1, 3, and 7. Scanning electron microscopy was used to analyze cell morphology at day 7. Cell differentiation was evaluated assaying alkaline phosphatase (ALP) activity at day 7, 14, and 21. Real-time PCR was used to evaluate the mRNA expression of periostin, ALP, type I collagen, bone sialoprotein and BMP-2. A commercially available enzyme-linked immunosorbent assay was used to assess BMP-2 production. Surface analysis disclosed excellent cell attachment, spread, and penetration into the porous scaffolds. The MTT assay indicated that scaffolds loaded with low concentration of BMP-2 did not influence the viability of cells. Cells grown on the modified scaffolds expressed higher levels of osteogenic markers than the nonmodified scaffolds (p<0.05). Poly(LLA-co-CL) and poly(LLA-co-DXO) scaffolds loaded with low-dose BMP-2 exhibited a significant effect stimulating PDL differentiation suggesting a continued evaluation in relevant in vivo models.

Original languageEnglish (US)
Pages (from-to)1991-1998
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume103
Issue number6
DOIs
StatePublished - Jun 1 2015

Fingerprint

Bone Morphogenetic Protein 2
Periodontal Ligament
Biodegradable polymers
Ligaments
Scaffolds (biology)
Scaffolds
Cell Differentiation
Polymers
Bone
Proteins
Assays
Cells
Alkaline Phosphatase
Phosphatases
Cell Survival
Integrin-Binding Sialoprotein
Bone and Bones
Immunosorbents
Bioelectric potentials
Tissue Engineering

Keywords

  • BMP-2
  • bioengineering
  • cell biology
  • differentiation
  • polymer scaffolds

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Skodje, A., Idris, S. B. M., Sun, Y., Bartaula, S., Mustafa, K., Finne-Wistrand, A., ... Leknes, K. N. (2015). Biodegradable polymer scaffolds loaded with low-dose BMP-2 stimulate periodontal ligament cell differentiation. Journal of Biomedical Materials Research - Part A, 103(6), 1991-1998. https://doi.org/10.1002/jbm.a.35334

Biodegradable polymer scaffolds loaded with low-dose BMP-2 stimulate periodontal ligament cell differentiation. / Skodje, Anders; Idris, Shaza Bushra M; Sun, Yang; Bartaula, Sushma; Mustafa, Kamal; Finne-Wistrand, Anna; Wikesjö, Ulf M E; Leknes, Knut N.

In: Journal of Biomedical Materials Research - Part A, Vol. 103, No. 6, 01.06.2015, p. 1991-1998.

Research output: Contribution to journalArticle

Skodje, A, Idris, SBM, Sun, Y, Bartaula, S, Mustafa, K, Finne-Wistrand, A, Wikesjö, UME & Leknes, KN 2015, 'Biodegradable polymer scaffolds loaded with low-dose BMP-2 stimulate periodontal ligament cell differentiation', Journal of Biomedical Materials Research - Part A, vol. 103, no. 6, pp. 1991-1998. https://doi.org/10.1002/jbm.a.35334
Skodje, Anders ; Idris, Shaza Bushra M ; Sun, Yang ; Bartaula, Sushma ; Mustafa, Kamal ; Finne-Wistrand, Anna ; Wikesjö, Ulf M E ; Leknes, Knut N. / Biodegradable polymer scaffolds loaded with low-dose BMP-2 stimulate periodontal ligament cell differentiation. In: Journal of Biomedical Materials Research - Part A. 2015 ; Vol. 103, No. 6. pp. 1991-1998.
@article{d5012f727f7943cdb65f5a7afadbe90d,
title = "Biodegradable polymer scaffolds loaded with low-dose BMP-2 stimulate periodontal ligament cell differentiation",
abstract = "Poly(l-lactide)-co-(epsilon-caprolactone) [poly(LLA-co-CL)] and poly(l-lactide)-co-(1,5-dioxepan-2-one) [poly(LLA-co-DXO)] are being considered candidate scaffolds for bone tissue engineering. We evaluated the bioactive potential of poly(LLA-co-CL) and poly(LLA-co-DXO) scaffolds loaded with low-dose bone morphogenetic protein-2 (BMP-2). Periodontal ligament (PDL) cells were cultured onto the various scaffolds loaded with 1 μg BMP-2 or without BMP-2 (control). Cell viability, attachment, and proliferation were determined using a methylthiazol tetrazolium (MTT) colorimetric assay at day 1, 3, and 7. Scanning electron microscopy was used to analyze cell morphology at day 7. Cell differentiation was evaluated assaying alkaline phosphatase (ALP) activity at day 7, 14, and 21. Real-time PCR was used to evaluate the mRNA expression of periostin, ALP, type I collagen, bone sialoprotein and BMP-2. A commercially available enzyme-linked immunosorbent assay was used to assess BMP-2 production. Surface analysis disclosed excellent cell attachment, spread, and penetration into the porous scaffolds. The MTT assay indicated that scaffolds loaded with low concentration of BMP-2 did not influence the viability of cells. Cells grown on the modified scaffolds expressed higher levels of osteogenic markers than the nonmodified scaffolds (p<0.05). Poly(LLA-co-CL) and poly(LLA-co-DXO) scaffolds loaded with low-dose BMP-2 exhibited a significant effect stimulating PDL differentiation suggesting a continued evaluation in relevant in vivo models.",
keywords = "BMP-2, bioengineering, cell biology, differentiation, polymer scaffolds",
author = "Anders Skodje and Idris, {Shaza Bushra M} and Yang Sun and Sushma Bartaula and Kamal Mustafa and Anna Finne-Wistrand and Wikesj{\"o}, {Ulf M E} and Leknes, {Knut N.}",
year = "2015",
month = "6",
day = "1",
doi = "10.1002/jbm.a.35334",
language = "English (US)",
volume = "103",
pages = "1991--1998",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "0021-9304",
publisher = "Heterocorporation",
number = "6",

}

TY - JOUR

T1 - Biodegradable polymer scaffolds loaded with low-dose BMP-2 stimulate periodontal ligament cell differentiation

AU - Skodje, Anders

AU - Idris, Shaza Bushra M

AU - Sun, Yang

AU - Bartaula, Sushma

AU - Mustafa, Kamal

AU - Finne-Wistrand, Anna

AU - Wikesjö, Ulf M E

AU - Leknes, Knut N.

PY - 2015/6/1

Y1 - 2015/6/1

N2 - Poly(l-lactide)-co-(epsilon-caprolactone) [poly(LLA-co-CL)] and poly(l-lactide)-co-(1,5-dioxepan-2-one) [poly(LLA-co-DXO)] are being considered candidate scaffolds for bone tissue engineering. We evaluated the bioactive potential of poly(LLA-co-CL) and poly(LLA-co-DXO) scaffolds loaded with low-dose bone morphogenetic protein-2 (BMP-2). Periodontal ligament (PDL) cells were cultured onto the various scaffolds loaded with 1 μg BMP-2 or without BMP-2 (control). Cell viability, attachment, and proliferation were determined using a methylthiazol tetrazolium (MTT) colorimetric assay at day 1, 3, and 7. Scanning electron microscopy was used to analyze cell morphology at day 7. Cell differentiation was evaluated assaying alkaline phosphatase (ALP) activity at day 7, 14, and 21. Real-time PCR was used to evaluate the mRNA expression of periostin, ALP, type I collagen, bone sialoprotein and BMP-2. A commercially available enzyme-linked immunosorbent assay was used to assess BMP-2 production. Surface analysis disclosed excellent cell attachment, spread, and penetration into the porous scaffolds. The MTT assay indicated that scaffolds loaded with low concentration of BMP-2 did not influence the viability of cells. Cells grown on the modified scaffolds expressed higher levels of osteogenic markers than the nonmodified scaffolds (p<0.05). Poly(LLA-co-CL) and poly(LLA-co-DXO) scaffolds loaded with low-dose BMP-2 exhibited a significant effect stimulating PDL differentiation suggesting a continued evaluation in relevant in vivo models.

AB - Poly(l-lactide)-co-(epsilon-caprolactone) [poly(LLA-co-CL)] and poly(l-lactide)-co-(1,5-dioxepan-2-one) [poly(LLA-co-DXO)] are being considered candidate scaffolds for bone tissue engineering. We evaluated the bioactive potential of poly(LLA-co-CL) and poly(LLA-co-DXO) scaffolds loaded with low-dose bone morphogenetic protein-2 (BMP-2). Periodontal ligament (PDL) cells were cultured onto the various scaffolds loaded with 1 μg BMP-2 or without BMP-2 (control). Cell viability, attachment, and proliferation were determined using a methylthiazol tetrazolium (MTT) colorimetric assay at day 1, 3, and 7. Scanning electron microscopy was used to analyze cell morphology at day 7. Cell differentiation was evaluated assaying alkaline phosphatase (ALP) activity at day 7, 14, and 21. Real-time PCR was used to evaluate the mRNA expression of periostin, ALP, type I collagen, bone sialoprotein and BMP-2. A commercially available enzyme-linked immunosorbent assay was used to assess BMP-2 production. Surface analysis disclosed excellent cell attachment, spread, and penetration into the porous scaffolds. The MTT assay indicated that scaffolds loaded with low concentration of BMP-2 did not influence the viability of cells. Cells grown on the modified scaffolds expressed higher levels of osteogenic markers than the nonmodified scaffolds (p<0.05). Poly(LLA-co-CL) and poly(LLA-co-DXO) scaffolds loaded with low-dose BMP-2 exhibited a significant effect stimulating PDL differentiation suggesting a continued evaluation in relevant in vivo models.

KW - BMP-2

KW - bioengineering

KW - cell biology

KW - differentiation

KW - polymer scaffolds

UR - http://www.scopus.com/inward/record.url?scp=84928584903&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928584903&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.35334

DO - 10.1002/jbm.a.35334

M3 - Article

VL - 103

SP - 1991

EP - 1998

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 0021-9304

IS - 6

ER -