TY - JOUR
T1 - Biofilm formation of clinically important microorganisms on 2D and 3D poly (methyl methacrylate) substrates
T2 - A surface-enhanced Raman scattering study
AU - Keleştemur, Seda
AU - Çobandede, Zehra
AU - Çulha, Mustafa
N1 - Funding Information:
The authors acknowledge the financial support of The Scientific and Technological Research Council of Turkey (TUBITAK Project no: 214Z129) and Yeditepe University. We also acknowledge the support of the COST action BM1401 European Network on Raman-Based Applications for Clinical Diagnostics (Raman4clinics).
Funding Information:
The authors acknowledge the financial support of The Scientific and Technological Research Council of Turkey (TUBITAK Project no: 214Z129) and Yeditepe University . We also acknowledge the support of the COST action BM1401 European Network on Raman-Based Applications for Clinical Diagnostics (Raman4clinics). Appendix A
Publisher Copyright:
© 2019
PY - 2020/4
Y1 - 2020/4
N2 - Clinically relevant microorganisms threaten patient's health often through biofilm formation on polymeric medical devices and implants. Poly (methyl methacrylate) is a commonly used polymer in medical implants and dental devices. In this study, biofilm characteristics of model microorganisms, Pseudomonas aeruginosa, Staphylococcus epidermidis and Candida albicans, were investigated at molecular level on 2-dimensional (2D) and 3-dimensional (3D) PMMA substrates to understand the influence of surface structures on biofilm formation and also to demonstrate the discrimination of microorganisms according to their metabolic activities by utilizing surface-enhanced Raman scattering (SERS). It was found that the fibrous 3D structure enhanced the assembly of microorganisms and enriched the biofilm structure while smooth polymeric surface decreased the biofilm formation rate and variety of biofilm content. Among the studied microorganisms, Pseudomonas aeruginosa and Candida albicans had a higher tendency to form biofilm on both 2D and 3D PMMA substrates. Although Staphlylococcus epidermidis showed slow adaption on PMMA surfaces, the 3D porous surfaces increased its biofilm formation rate significantly compared to 2D surface.
AB - Clinically relevant microorganisms threaten patient's health often through biofilm formation on polymeric medical devices and implants. Poly (methyl methacrylate) is a commonly used polymer in medical implants and dental devices. In this study, biofilm characteristics of model microorganisms, Pseudomonas aeruginosa, Staphylococcus epidermidis and Candida albicans, were investigated at molecular level on 2-dimensional (2D) and 3-dimensional (3D) PMMA substrates to understand the influence of surface structures on biofilm formation and also to demonstrate the discrimination of microorganisms according to their metabolic activities by utilizing surface-enhanced Raman scattering (SERS). It was found that the fibrous 3D structure enhanced the assembly of microorganisms and enriched the biofilm structure while smooth polymeric surface decreased the biofilm formation rate and variety of biofilm content. Among the studied microorganisms, Pseudomonas aeruginosa and Candida albicans had a higher tendency to form biofilm on both 2D and 3D PMMA substrates. Although Staphlylococcus epidermidis showed slow adaption on PMMA surfaces, the 3D porous surfaces increased its biofilm formation rate significantly compared to 2D surface.
KW - Bacteria
KW - Biofilm formation
KW - Poly (methyl methacrylate)
KW - SERS
KW - Yeast
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U2 - 10.1016/j.colsurfb.2019.110765
DO - 10.1016/j.colsurfb.2019.110765
M3 - Article
C2 - 31955016
AN - SCOPUS:85077795215
SN - 0927-7765
VL - 188
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
M1 - 110765
ER -