Biofilm formation of clinically important microorganisms on 2D and 3D poly (methyl methacrylate) substrates: A surface-enhanced Raman scattering study

Seda Keleştemur, Zehra Çobandede, Mustafa Çulha

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Clinically relevant microorganisms threaten patient's health often through biofilm formation on polymeric medical devices and implants. Poly (methyl methacrylate) is a commonly used polymer in medical implants and dental devices. In this study, biofilm characteristics of model microorganisms, Pseudomonas aeruginosa, Staphylococcus epidermidis and Candida albicans, were investigated at molecular level on 2-dimensional (2D) and 3-dimensional (3D) PMMA substrates to understand the influence of surface structures on biofilm formation and also to demonstrate the discrimination of microorganisms according to their metabolic activities by utilizing surface-enhanced Raman scattering (SERS). It was found that the fibrous 3D structure enhanced the assembly of microorganisms and enriched the biofilm structure while smooth polymeric surface decreased the biofilm formation rate and variety of biofilm content. Among the studied microorganisms, Pseudomonas aeruginosa and Candida albicans had a higher tendency to form biofilm on both 2D and 3D PMMA substrates. Although Staphlylococcus epidermidis showed slow adaption on PMMA surfaces, the 3D porous surfaces increased its biofilm formation rate significantly compared to 2D surface.

Original languageEnglish (US)
Article number110765
JournalColloids and Surfaces B: Biointerfaces
Volume188
DOIs
StatePublished - Apr 2020
Externally publishedYes

Keywords

  • Bacteria
  • Biofilm formation
  • Poly (methyl methacrylate)
  • SERS
  • Yeast

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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