Bioinformatic analysis of circadian gene oscillation in mouse aorta

R. Daniel Rudic, Peter McNamara, Dermot Reilly, Tilo Grosser, Anne Marie Curtis, Thomas S. Price, Satchidananda Panda, John B. Hogenesch, Garret A. FitzGerald

Research output: Contribution to journalArticle

104 Scopus citations

Abstract

Background - Circadian rhythmicity of many aspects of cardiovascular function-blood pressure, coagulation and contractile function-is well established, as is diurnal variation in important clinical events, such as myocardial infarction and stroke. Here, we undertake studies to globally assess circadian gene expression in murine aorta. Methods and Results - Aortae from mice were harvested at 4-hour intervals for 2 circadian cycles (48 hours). Gene expression was assessed by expression profiling and subjected to a gene ontology bioinformatics analysis. Three hundred thirty transcripts exhibited a circadian pattern of oscillation in mouse aorta, including those intrinsic to the function of the molecular clock. In addition, many genes relevant to protein folding, protein degradation, glucose and lipid metabolism, adipocyte maturation, vascular integrity, and the response to injury are also included in this subset of roughly 7000 genes screened for circadian oscillation. Conclusions - Detection of functional cassettes of vascular genes that exhibit circadian regulation in the mouse will facilitate elucidation of the mechanisms by which the molecular clock may interact with environmental variables to modulate cardiovascular function and the response to therapeutic interventions.

Original languageEnglish (US)
Pages (from-to)2716-2724
Number of pages9
JournalCirculation
Volume112
Issue number17
DOIs
StatePublished - Oct 25 2005
Externally publishedYes

Keywords

  • Aorta
  • Circadian rhythm
  • Genes
  • Metabolism
  • Vasculature

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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  • Cite this

    Rudic, R. D., McNamara, P., Reilly, D., Grosser, T., Curtis, A. M., Price, T. S., Panda, S., Hogenesch, J. B., & FitzGerald, G. A. (2005). Bioinformatic analysis of circadian gene oscillation in mouse aorta. Circulation, 112(17), 2716-2724. https://doi.org/10.1161/CIRCULATIONAHA.105.568626