Biological effects of polychromatic light

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Predicting the effects of polychromatic light on biological systems is a central goal of environmental photobiology. If the dose-response function for a process is a linear function of the light incident on a system at each wavelength within the spectrum, the effect of a polychromatic spectrum is obtained by integrating the product of the cross section for the reaction at each wavelength and the spectral irradiance at that wavelength over both wavelength and time. This procedure cannot be used, however, if the dose-response functions for an effect are not linear functions of photon dose. Although many photochemical reactions are linear within the biologically relevant range of doses, many biological end points are not. I describe procedures for calculating the effects of polychromatic irradiations on systems that exhibit certain classes of dose-response functions, including power law responses typical of mutation induction and exponential dose-responses typical of cell survival. I also present an approach to predict the effects of polychromatic spectra on systems in which the ultraviolet components form pyrimidine dimers, and the longer-wavelength ultraviolet and visible components remove them by photoreactivation, thus generating complex dose-response functions for these coupled light-driven reactions.

Original languageEnglish (US)
Pages (from-to)164-170
Number of pages7
JournalPhotochemistry and Photobiology
Volume76
Issue number2
DOIs
StatePublished - Aug 1 2002
Externally publishedYes

Fingerprint

biological effects
Light
dosage
Wavelength
Photobiology
Pyrimidine Dimers
Cross Reactions
wavelengths
Photons
Dosimetry
Cell Survival
Mutation
Photochemical reactions
Biological systems
pyrimidines
mutations
irradiance
photochemical reactions
Cells
Irradiation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Biological effects of polychromatic light. / Sutherland, John C.

In: Photochemistry and Photobiology, Vol. 76, No. 2, 01.08.2002, p. 164-170.

Research output: Contribution to journalArticle

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