Biological evaluations of zinc hexyl vanillate cement using two in vivo test methods

J. C. Keller, Barry Dale Hammond, K. K. Kowalyk, G. M. Brauer

Research output: Contribution to journalArticle

Abstract

The cellular and tissue responses to 3 dental cements were studied by 2 methodologies, the connective tissue implantation technique (CTI), recommended by the ADA, and the peritoneal cavity implantation technique (PCI), which has emerged as a method to quantitatively study the cellular response to implanted materials. A mixed inflammatory cell response consisting of neutrophils and macrophages occurred at most of the time periods for all the cements examined in the CTI study. Similar cell populations, including lymphocytes, were observed with the PCI study using differential staining techniques. The histopathological observations from both procedures were similar. Each cement elicited a chronic foreign body reaction containing few chronic cells and mature fibroblastic activity. The intensity of the observed tissue reactions for each cement, as determined by capsule thickness in the CTI study and the overall cell counts in the PCI study, did not differ significantly with increasing time. The cellular and connective tissue reactions to the zinc hexyl vanillate (ZHV) cement approximated those of zinc oxide-eugenol (ZOE) and zinc phosphate (ZP) cements for both methodologies utilized in this work. While similar histopathological results were obtained for the implantation of cements using both methodologies, the PCI technique offers a more thorough investigation of cellular and tissue responses to implanted materials. In addition to histopathological evaluations, the PCI techniques allows quantitative investigation of the specific cells responding to the implants, and provides a mechanism, using chemical analysis techniques, to quantify the concentration of specific degradative products within the retrieved cells and host tissue. Finally, the results from these 2 methodologies demonstrated the acceptable biological performance of ZHV cement compared with the clinically acceptable ZP and ZOE cement formulations.

Original languageEnglish (US)
Pages (from-to)341-350
Number of pages10
JournalDental Materials
Volume4
Issue number6
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

Fingerprint

Peritoneal Cavity
Zinc
Cements
Connective Tissue
Tissue
Zinc Oxide-Eugenol Cement
Zinc Phosphate Cement
Dental Cements
Zinc Oxide
Foreign-Body Reaction
Eugenol
Zinc oxide
Phosphates
Dental cement
Capsules
Neutrophils
Cell Count
Macrophages
Lymphocytes
Staining and Labeling

Keywords

  • dental cement
  • zinc hexyl vanillate
  • zinc oxide-eugenol
  • zinc phosphate

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Biological evaluations of zinc hexyl vanillate cement using two in vivo test methods. / Keller, J. C.; Hammond, Barry Dale; Kowalyk, K. K.; Brauer, G. M.

In: Dental Materials, Vol. 4, No. 6, 01.01.1988, p. 341-350.

Research output: Contribution to journalArticle

Keller, J. C. ; Hammond, Barry Dale ; Kowalyk, K. K. ; Brauer, G. M. / Biological evaluations of zinc hexyl vanillate cement using two in vivo test methods. In: Dental Materials. 1988 ; Vol. 4, No. 6. pp. 341-350.
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