Biomimetic analogs for collagen biomineralization

L. Gu, Y. K. Kim, Y. Liu, H. Ryou, C. E. Wimmer, L. Dai, D. D. Arola, Stephen Warwick Looney, David Henry Pashley, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Inability of chemical phosphorylation of sodium trimetaphosphate to induce intrafibrillar mineralization of type I collagen may be due to the failure to incorporate a biomimetic analog to stabilize amorphous calcium phosphates (ACP) as nanoprecursors. This study investigated adsorption/desorption characteristics of hydrolyzed and pH-adjusted sodium trimetaphosphate (HPA-Na3P 3O9) to collagen. Based on those results, a 5-minute treatment time with 2.8 wt% HPA-Na3P3O9 was used in a single-layer reconstituted collagen model to confirm that both the ACP-stabilization analog and matrix phosphoprotein analog must be present for intrafibrillar mineralization. The results of that model were further validated by complete remineralization of phosphoric-acid-etched dentin treated with the matrix phosphoprotein analog and lined with a remineralizing lining composite, and with the ACP-stabilization analog supplied in simulated body fluid. An understanding of the basic processes involved in intrafibrillar mineralization of reconstituted collagen fibrils facilitates the design of novel tissue engineering materials for hard tissue repair and regeneration.

Original languageEnglish (US)
Pages (from-to)82-87
Number of pages6
JournalJournal of Dental Research
Volume90
Issue number1
DOIs
StatePublished - Jan 1 2011

Fingerprint

Biomimetics
Collagen
Phosphoproteins
Body Fluids
Dentin
Tissue Engineering
Collagen Type I
Adsorption
Regeneration
Phosphorylation
amorphous calcium phosphate
trimetaphosphoric acid

Keywords

  • biomimetic
  • biomineralization
  • chemical phosphorylation
  • collagen
  • specific binding

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Biomimetic analogs for collagen biomineralization. / Gu, L.; Kim, Y. K.; Liu, Y.; Ryou, H.; Wimmer, C. E.; Dai, L.; Arola, D. D.; Looney, Stephen Warwick; Pashley, David Henry; Tay, Franklin Chi Meng.

In: Journal of Dental Research, Vol. 90, No. 1, 01.01.2011, p. 82-87.

Research output: Contribution to journalArticle

Gu, L, Kim, YK, Liu, Y, Ryou, H, Wimmer, CE, Dai, L, Arola, DD, Looney, SW, Pashley, DH & Tay, FCM 2011, 'Biomimetic analogs for collagen biomineralization', Journal of Dental Research, vol. 90, no. 1, pp. 82-87. https://doi.org/10.1177/0022034510385241
Gu L, Kim YK, Liu Y, Ryou H, Wimmer CE, Dai L et al. Biomimetic analogs for collagen biomineralization. Journal of Dental Research. 2011 Jan 1;90(1):82-87. https://doi.org/10.1177/0022034510385241
Gu, L. ; Kim, Y. K. ; Liu, Y. ; Ryou, H. ; Wimmer, C. E. ; Dai, L. ; Arola, D. D. ; Looney, Stephen Warwick ; Pashley, David Henry ; Tay, Franklin Chi Meng. / Biomimetic analogs for collagen biomineralization. In: Journal of Dental Research. 2011 ; Vol. 90, No. 1. pp. 82-87.
@article{b7e35d0c6d9d47559f08fb00dee86b4b,
title = "Biomimetic analogs for collagen biomineralization",
abstract = "Inability of chemical phosphorylation of sodium trimetaphosphate to induce intrafibrillar mineralization of type I collagen may be due to the failure to incorporate a biomimetic analog to stabilize amorphous calcium phosphates (ACP) as nanoprecursors. This study investigated adsorption/desorption characteristics of hydrolyzed and pH-adjusted sodium trimetaphosphate (HPA-Na3P 3O9) to collagen. Based on those results, a 5-minute treatment time with 2.8 wt{\%} HPA-Na3P3O9 was used in a single-layer reconstituted collagen model to confirm that both the ACP-stabilization analog and matrix phosphoprotein analog must be present for intrafibrillar mineralization. The results of that model were further validated by complete remineralization of phosphoric-acid-etched dentin treated with the matrix phosphoprotein analog and lined with a remineralizing lining composite, and with the ACP-stabilization analog supplied in simulated body fluid. An understanding of the basic processes involved in intrafibrillar mineralization of reconstituted collagen fibrils facilitates the design of novel tissue engineering materials for hard tissue repair and regeneration.",
keywords = "biomimetic, biomineralization, chemical phosphorylation, collagen, specific binding",
author = "L. Gu and Kim, {Y. K.} and Y. Liu and H. Ryou and Wimmer, {C. E.} and L. Dai and Arola, {D. D.} and Looney, {Stephen Warwick} and Pashley, {David Henry} and Tay, {Franklin Chi Meng}",
year = "2011",
month = "1",
day = "1",
doi = "10.1177/0022034510385241",
language = "English (US)",
volume = "90",
pages = "82--87",
journal = "Journal of Dental Research",
issn = "0022-0345",
publisher = "SAGE Publications Inc.",
number = "1",

}

TY - JOUR

T1 - Biomimetic analogs for collagen biomineralization

AU - Gu, L.

AU - Kim, Y. K.

AU - Liu, Y.

AU - Ryou, H.

AU - Wimmer, C. E.

AU - Dai, L.

AU - Arola, D. D.

AU - Looney, Stephen Warwick

AU - Pashley, David Henry

AU - Tay, Franklin Chi Meng

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Inability of chemical phosphorylation of sodium trimetaphosphate to induce intrafibrillar mineralization of type I collagen may be due to the failure to incorporate a biomimetic analog to stabilize amorphous calcium phosphates (ACP) as nanoprecursors. This study investigated adsorption/desorption characteristics of hydrolyzed and pH-adjusted sodium trimetaphosphate (HPA-Na3P 3O9) to collagen. Based on those results, a 5-minute treatment time with 2.8 wt% HPA-Na3P3O9 was used in a single-layer reconstituted collagen model to confirm that both the ACP-stabilization analog and matrix phosphoprotein analog must be present for intrafibrillar mineralization. The results of that model were further validated by complete remineralization of phosphoric-acid-etched dentin treated with the matrix phosphoprotein analog and lined with a remineralizing lining composite, and with the ACP-stabilization analog supplied in simulated body fluid. An understanding of the basic processes involved in intrafibrillar mineralization of reconstituted collagen fibrils facilitates the design of novel tissue engineering materials for hard tissue repair and regeneration.

AB - Inability of chemical phosphorylation of sodium trimetaphosphate to induce intrafibrillar mineralization of type I collagen may be due to the failure to incorporate a biomimetic analog to stabilize amorphous calcium phosphates (ACP) as nanoprecursors. This study investigated adsorption/desorption characteristics of hydrolyzed and pH-adjusted sodium trimetaphosphate (HPA-Na3P 3O9) to collagen. Based on those results, a 5-minute treatment time with 2.8 wt% HPA-Na3P3O9 was used in a single-layer reconstituted collagen model to confirm that both the ACP-stabilization analog and matrix phosphoprotein analog must be present for intrafibrillar mineralization. The results of that model were further validated by complete remineralization of phosphoric-acid-etched dentin treated with the matrix phosphoprotein analog and lined with a remineralizing lining composite, and with the ACP-stabilization analog supplied in simulated body fluid. An understanding of the basic processes involved in intrafibrillar mineralization of reconstituted collagen fibrils facilitates the design of novel tissue engineering materials for hard tissue repair and regeneration.

KW - biomimetic

KW - biomineralization

KW - chemical phosphorylation

KW - collagen

KW - specific binding

UR - http://www.scopus.com/inward/record.url?scp=78650972269&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650972269&partnerID=8YFLogxK

U2 - 10.1177/0022034510385241

DO - 10.1177/0022034510385241

M3 - Article

C2 - 20940362

AN - SCOPUS:78650972269

VL - 90

SP - 82

EP - 87

JO - Journal of Dental Research

JF - Journal of Dental Research

SN - 0022-0345

IS - 1

ER -