Biomimetic remineralization as a progressive dehydration mechanism of collagen matrices - Implications in the aging of resin-dentin bonds

Young Kyung Kim, Sui Mai, Annalisa Mazzoni, Yan Liu, Arzu Tezvergil-Mutluay, Kei Takahashi, Kai Zhang, David H. Pashley, Franklin R. Tay

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Biomineralization is a dehydration process in which water from the intrafibrillar compartments of collagen fibrils are progressively replaced by apatites. As water is an important element that induces a lack of durability of resin-dentin bonds, this study has examined the use of a biomimetic remineralization strategy as a progressive dehydration mechanism to preserve joint integrity and maintain adhesive strength after ageing. Human dentin surfaces were bonded with dentin adhesives, restored with resin composites and sectioned into sticks containing the adhesive joint. Experimental specimens were aged in a biomimetic analog-containing remineralizing medium and control specimens in simulated body fluid for up to 12 months. Specimens retrieved after the designated periods were examined by transmission electron microscopy for the presence of water-rich regions using a silver tracer and for collagen degradation within the adhesive joints. Tensile testing was performed to determine the potential loss of bond integrity after ageing. Control specimens exhibited severe collagen degradation within the adhesive joint after ageing. Remineralized specimens exhibited progressive dehydration, as manifested by silver tracer reduction and partial remineralization of water-filled microchannels within the adhesive joint, as well as intrafibrillar remineralization of collagen fibrils that were demineralized initially as part of the bonding procedure. Biomimetic remineralization as a progressive dehydration mechanism of water-rich, resin-sparse collagen matrices enables these adhesive joints to resist degradation over a 12-month ageing period, as verified by the conservation of their tensile bond strength. The ability of the proof of concept biomimetic remineralization strategy to prevent bond degradation warrants further development of clinically relevant delivery systems.

Original languageEnglish (US)
Pages (from-to)3729-3739
Number of pages11
JournalActa biomaterialia
Volume6
Issue number9
DOIs
StatePublished - Jan 1 2010

Fingerprint

Biomimetics
Adhesive joints
Dentin
Dehydration
Collagen
Adhesives
Resins
Aging of materials
Joints
Water
Degradation
Silver
Biomineralization
Apatites
Composite Resins
Body fluids
Tensile testing
Apatite
Microchannels
Conservation

Keywords

  • Biomimetics
  • Dehydration
  • Dentin adhesive
  • Intrafibrillar
  • Remineralization

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Biomimetic remineralization as a progressive dehydration mechanism of collagen matrices - Implications in the aging of resin-dentin bonds. / Kim, Young Kyung; Mai, Sui; Mazzoni, Annalisa; Liu, Yan; Tezvergil-Mutluay, Arzu; Takahashi, Kei; Zhang, Kai; Pashley, David H.; Tay, Franklin R.

In: Acta biomaterialia, Vol. 6, No. 9, 01.01.2010, p. 3729-3739.

Research output: Contribution to journalArticle

Kim, Young Kyung ; Mai, Sui ; Mazzoni, Annalisa ; Liu, Yan ; Tezvergil-Mutluay, Arzu ; Takahashi, Kei ; Zhang, Kai ; Pashley, David H. ; Tay, Franklin R. / Biomimetic remineralization as a progressive dehydration mechanism of collagen matrices - Implications in the aging of resin-dentin bonds. In: Acta biomaterialia. 2010 ; Vol. 6, No. 9. pp. 3729-3739.
@article{9e64f4dffc934fd1a786bab44d95584d,
title = "Biomimetic remineralization as a progressive dehydration mechanism of collagen matrices - Implications in the aging of resin-dentin bonds",
abstract = "Biomineralization is a dehydration process in which water from the intrafibrillar compartments of collagen fibrils are progressively replaced by apatites. As water is an important element that induces a lack of durability of resin-dentin bonds, this study has examined the use of a biomimetic remineralization strategy as a progressive dehydration mechanism to preserve joint integrity and maintain adhesive strength after ageing. Human dentin surfaces were bonded with dentin adhesives, restored with resin composites and sectioned into sticks containing the adhesive joint. Experimental specimens were aged in a biomimetic analog-containing remineralizing medium and control specimens in simulated body fluid for up to 12 months. Specimens retrieved after the designated periods were examined by transmission electron microscopy for the presence of water-rich regions using a silver tracer and for collagen degradation within the adhesive joints. Tensile testing was performed to determine the potential loss of bond integrity after ageing. Control specimens exhibited severe collagen degradation within the adhesive joint after ageing. Remineralized specimens exhibited progressive dehydration, as manifested by silver tracer reduction and partial remineralization of water-filled microchannels within the adhesive joint, as well as intrafibrillar remineralization of collagen fibrils that were demineralized initially as part of the bonding procedure. Biomimetic remineralization as a progressive dehydration mechanism of water-rich, resin-sparse collagen matrices enables these adhesive joints to resist degradation over a 12-month ageing period, as verified by the conservation of their tensile bond strength. The ability of the proof of concept biomimetic remineralization strategy to prevent bond degradation warrants further development of clinically relevant delivery systems.",
keywords = "Biomimetics, Dehydration, Dentin adhesive, Intrafibrillar, Remineralization",
author = "Kim, {Young Kyung} and Sui Mai and Annalisa Mazzoni and Yan Liu and Arzu Tezvergil-Mutluay and Kei Takahashi and Kai Zhang and Pashley, {David H.} and Tay, {Franklin R.}",
year = "2010",
month = "1",
day = "1",
doi = "10.1016/j.actbio.2010.03.021",
language = "English (US)",
volume = "6",
pages = "3729--3739",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",
number = "9",

}

TY - JOUR

T1 - Biomimetic remineralization as a progressive dehydration mechanism of collagen matrices - Implications in the aging of resin-dentin bonds

AU - Kim, Young Kyung

AU - Mai, Sui

AU - Mazzoni, Annalisa

AU - Liu, Yan

AU - Tezvergil-Mutluay, Arzu

AU - Takahashi, Kei

AU - Zhang, Kai

AU - Pashley, David H.

AU - Tay, Franklin R.

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Biomineralization is a dehydration process in which water from the intrafibrillar compartments of collagen fibrils are progressively replaced by apatites. As water is an important element that induces a lack of durability of resin-dentin bonds, this study has examined the use of a biomimetic remineralization strategy as a progressive dehydration mechanism to preserve joint integrity and maintain adhesive strength after ageing. Human dentin surfaces were bonded with dentin adhesives, restored with resin composites and sectioned into sticks containing the adhesive joint. Experimental specimens were aged in a biomimetic analog-containing remineralizing medium and control specimens in simulated body fluid for up to 12 months. Specimens retrieved after the designated periods were examined by transmission electron microscopy for the presence of water-rich regions using a silver tracer and for collagen degradation within the adhesive joints. Tensile testing was performed to determine the potential loss of bond integrity after ageing. Control specimens exhibited severe collagen degradation within the adhesive joint after ageing. Remineralized specimens exhibited progressive dehydration, as manifested by silver tracer reduction and partial remineralization of water-filled microchannels within the adhesive joint, as well as intrafibrillar remineralization of collagen fibrils that were demineralized initially as part of the bonding procedure. Biomimetic remineralization as a progressive dehydration mechanism of water-rich, resin-sparse collagen matrices enables these adhesive joints to resist degradation over a 12-month ageing period, as verified by the conservation of their tensile bond strength. The ability of the proof of concept biomimetic remineralization strategy to prevent bond degradation warrants further development of clinically relevant delivery systems.

AB - Biomineralization is a dehydration process in which water from the intrafibrillar compartments of collagen fibrils are progressively replaced by apatites. As water is an important element that induces a lack of durability of resin-dentin bonds, this study has examined the use of a biomimetic remineralization strategy as a progressive dehydration mechanism to preserve joint integrity and maintain adhesive strength after ageing. Human dentin surfaces were bonded with dentin adhesives, restored with resin composites and sectioned into sticks containing the adhesive joint. Experimental specimens were aged in a biomimetic analog-containing remineralizing medium and control specimens in simulated body fluid for up to 12 months. Specimens retrieved after the designated periods were examined by transmission electron microscopy for the presence of water-rich regions using a silver tracer and for collagen degradation within the adhesive joints. Tensile testing was performed to determine the potential loss of bond integrity after ageing. Control specimens exhibited severe collagen degradation within the adhesive joint after ageing. Remineralized specimens exhibited progressive dehydration, as manifested by silver tracer reduction and partial remineralization of water-filled microchannels within the adhesive joint, as well as intrafibrillar remineralization of collagen fibrils that were demineralized initially as part of the bonding procedure. Biomimetic remineralization as a progressive dehydration mechanism of water-rich, resin-sparse collagen matrices enables these adhesive joints to resist degradation over a 12-month ageing period, as verified by the conservation of their tensile bond strength. The ability of the proof of concept biomimetic remineralization strategy to prevent bond degradation warrants further development of clinically relevant delivery systems.

KW - Biomimetics

KW - Dehydration

KW - Dentin adhesive

KW - Intrafibrillar

KW - Remineralization

UR - http://www.scopus.com/inward/record.url?scp=77956727114&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956727114&partnerID=8YFLogxK

U2 - 10.1016/j.actbio.2010.03.021

DO - 10.1016/j.actbio.2010.03.021

M3 - Article

C2 - 20304110

AN - SCOPUS:77956727114

VL - 6

SP - 3729

EP - 3739

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

IS - 9

ER -