Biosynthesis of glycosphingolipids is reduced in the absence of a vimentin intermediate filament network

B. K. Gillard, L. T. Thurmon, R. G. Harrell, Y. Capetanaki, M. Saito, Robert K Yu, D. M. Marcus

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Our previous observations on the immunocytochemical colocalization of intermediate filaments and glycosphingolipids led us to analyze the role of filaments in the biosynthesis and intracellular transport of glycosphingolipids. Cells with (vim+) and without (vim-) vimentin intermediate filaments were cloned from the adrenal carcinoma cell line SW13. There was no difference between vim+ and vim- cells in the proportion of newly synthesized C6-NBD-glucosylceramide transported to the plasma membrane. The vim+ cells synthesized glycosphingolipids, especially lactosylceramide and globotriosylceramide, and to a lesser extent G(M3) ganglioside, more rapidly than vim- cells. The altered rate of biosynthesis did not result from differences in the levels of the glycosyltransferases that synthesize those compounds. To determine whether the presence of a vimentin network was responsible for the differences in biosynthesis, mouse vimentin cDNA was transfected into vim- cells. Transfected cells that expressed a mouse vimentin network demonstrated a twofold or greater increase in the rate of biosynthesis of neutral glycosphingolipids and gangliosides. There was no difference between vim+ and vim- cells in the synthesis of ceramide or sphingomyelin, or in their content of phospholipids or cholesterol. The nature of the biochemical defect(s) underlying the diminished incorporation of radiolabeled sugars into glycosphingolipids is unclear. Possibilities include alterations in the ultrastructure of the Golgi and/or abnormalities in a portion of the endocytic pathway.

Original languageEnglish (US)
Pages (from-to)3545-3555
Number of pages11
JournalJournal of Cell Science
Volume107
Issue number12
StatePublished - Dec 1 1994
Externally publishedYes

Fingerprint

Glycosphingolipids
Intermediate Filaments
Vimentin
Neutral Glycosphingolipids
G(M3) Ganglioside
Glucosylceramides
Glycosyltransferases
Sphingomyelins
Gangliosides
Ceramides
Phospholipids
Complementary DNA
Cholesterol
Cell Membrane
Carcinoma
Cell Line

Keywords

  • Glycosphingolipid biosynthesis
  • Intermediate filament function
  • Vimentin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Gillard, B. K., Thurmon, L. T., Harrell, R. G., Capetanaki, Y., Saito, M., Yu, R. K., & Marcus, D. M. (1994). Biosynthesis of glycosphingolipids is reduced in the absence of a vimentin intermediate filament network. Journal of Cell Science, 107(12), 3545-3555.

Biosynthesis of glycosphingolipids is reduced in the absence of a vimentin intermediate filament network. / Gillard, B. K.; Thurmon, L. T.; Harrell, R. G.; Capetanaki, Y.; Saito, M.; Yu, Robert K; Marcus, D. M.

In: Journal of Cell Science, Vol. 107, No. 12, 01.12.1994, p. 3545-3555.

Research output: Contribution to journalArticle

Gillard, BK, Thurmon, LT, Harrell, RG, Capetanaki, Y, Saito, M, Yu, RK & Marcus, DM 1994, 'Biosynthesis of glycosphingolipids is reduced in the absence of a vimentin intermediate filament network', Journal of Cell Science, vol. 107, no. 12, pp. 3545-3555.
Gillard BK, Thurmon LT, Harrell RG, Capetanaki Y, Saito M, Yu RK et al. Biosynthesis of glycosphingolipids is reduced in the absence of a vimentin intermediate filament network. Journal of Cell Science. 1994 Dec 1;107(12):3545-3555.
Gillard, B. K. ; Thurmon, L. T. ; Harrell, R. G. ; Capetanaki, Y. ; Saito, M. ; Yu, Robert K ; Marcus, D. M. / Biosynthesis of glycosphingolipids is reduced in the absence of a vimentin intermediate filament network. In: Journal of Cell Science. 1994 ; Vol. 107, No. 12. pp. 3545-3555.
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