Blocking myristoylation of Src inhibits its kinase activity and suppresses prostate cancer progression

Sungjin Kim, Omar Awad Alsaidan, Octavia Goodwin, Qianjin Li, Essilvo Sulejmani, Zhen Han, Aiping Bai, Thomas Albers, Zanna Beharry, Y. George Zheng, James S. Norris, Zdzislaw M. Szulc, Alicja Bielawska, Iryna Lebedyeva, Scott D. Pegan, Houjian Cai

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Protein N-myristoylation enables localization to membranes and helps maintain protein conformation and function. N-myristoyltransferases (NMT) catalyze co- or posttranslational myristoylation of Src family kinases and other oncogenic proteins, thereby regulating their function. In this study, we provide genetic and pharmacologic evidence that inhibiting the N-myristoyltransferase NMT1 suppresses cell-cycle progression, proliferation, and malignant growth of prostate cancer cells. Loss of myristoylation abolished the tumorigenic potential of Src and its synergy with androgen receptor in mediating tumor invasion. We identified the myristoyl-CoA analogue B13 as a small-molecule inhibitor of NMT1 enzymatic activity. B13 exposure blocked Src myristoylation and Src localization to the cytoplasmic membrane, attenuating Src-mediated oncogenic signaling. B13 exerted its anti-invasive and antitumor effects against prostate cancer cells, with minimal toxic side-effects in vivo. Structural optimization based on structure–activity relationships enabled the chemical synthesis of LCL204, with enhanced inhibitory potency against NMT1. Collectively, our results offer a preclinical proof of concept for the use of protein myristoylation inhibitors as a strategy to block prostate cancer progression.

Original languageEnglish (US)
Pages (from-to)6950-6952
Number of pages3
JournalCancer Research
Volume77
Issue number24
DOIs
StatePublished - Dec 15 2017

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Kim, S., Alsaidan, O. A., Goodwin, O., Li, Q., Sulejmani, E., Han, Z., Bai, A., Albers, T., Beharry, Z., Zheng, Y. G., Norris, J. S., Szulc, Z. M., Bielawska, A., Lebedyeva, I., Pegan, S. D., & Cai, H. (2017). Blocking myristoylation of Src inhibits its kinase activity and suppresses prostate cancer progression. Cancer Research, 77(24), 6950-6952. https://doi.org/10.1158/0008-5472.CAN-17-0981