Blood volume measurement: The comparison of pulse dye densitometry and Dill and Costill's methods

Eric Jacques Belin de Chantemele, Guillemette Gauquelin-Koch, Monique Duvareille, Natacha Pellet, Claude Gharib, Marc Antoine Custaud

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In many clinical situations, it is crucial to determine circulating blood volume (BV) easily and to repeat this measurement. The Dye DensitoGram Analyzer® (DDG, Nihon Kohden Corp) measures semi-automatically BV, using an injection of IndoCyanine Green (ICG, 10 mg), and avoiding intermittent blood samples. The DDG was used during a 90-day microgravity simulation by Head-Down-Tilt bed rest (HDT) to measure BV and compared with the calculation of the plasma volume (PV) variations according to Dill and Costill's formula (DC). Seventeen healthy volunteers were included: 8 control subjects (Co) and 9 subjects submitted to a resistive exercise counter-measure (CM). Measurements were performed, one day before HDT, on days 3 and 90 of HDT and on day 9 after HDT. A double measurement of the BV was performed to assess the repeatability of this method. On the last day of HDT a significant decrease (p < 0.05) in the PV was noted with the DDG (Co: - 12.3 ± 5.7%, CM: - 9.0 ± 5.3%) and DC; (Co: - 4.7 ± 1.8%, CM: - 6.8 ± 2.5%). A good repeatability of the technique was shown with a low intrasubjects coefficient of variation (4.95 ± 0.95%) and an acceptable intersubjects coefficient of variation (15.30 ± 1.13%). No correlation was noted between DDG and DC (r 2 = 0.27). The DDG gives a good repeatability, not affected by the microgravity exposure. Thanks to its capacity to measure accurately the BV within 7-10 min, this device presents major advantages for clinical use and research purpose.

Original languageEnglish (US)
Pages (from-to)1564-1569
Number of pages6
JournalLife sciences
Volume78
Issue number14
DOIs
StatePublished - Feb 28 2006

Fingerprint

Anethum graveolens
Volume measurement
Densitometry
Blood Volume
Blood
Coloring Agents
Plasma Volume
Microgravity
Weightlessness Simulation
Head-Down Tilt
Weightlessness
Bed Rest
Indocyanine Green
Plasmas
Healthy Volunteers
Exercise
Equipment and Supplies
Injections
Research

Keywords

  • Blood volume
  • Dye DensitoGram Analyzer®
  • Indocyanine green
  • Microgravity simulation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Blood volume measurement : The comparison of pulse dye densitometry and Dill and Costill's methods. / Belin de Chantemele, Eric Jacques; Gauquelin-Koch, Guillemette; Duvareille, Monique; Pellet, Natacha; Gharib, Claude; Custaud, Marc Antoine.

In: Life sciences, Vol. 78, No. 14, 28.02.2006, p. 1564-1569.

Research output: Contribution to journalArticle

Belin de Chantemele, Eric Jacques ; Gauquelin-Koch, Guillemette ; Duvareille, Monique ; Pellet, Natacha ; Gharib, Claude ; Custaud, Marc Antoine. / Blood volume measurement : The comparison of pulse dye densitometry and Dill and Costill's methods. In: Life sciences. 2006 ; Vol. 78, No. 14. pp. 1564-1569.
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