Blue light differentially modulates cell survival and growth

J. C. Wataha, J. B. Lewis, P. E. Lockwood, Stephen Hsu, Regina L W Messer, Frederick Rueggeberg, S. Bouillaguet

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Previous studies have reported that blue light (400-500 nm) inhibits cell mitochondrial activity. We investigated the hypothesis that cells with high energy consumption are most susceptible to blue-light-induced mitochondrial inhibition. We estimated cell energy consumption by population doubling time, and cell survival and growth by succinate dehydrogenase (SDH) activity. Six cell types were exposed to 5 or 60 J/cm2 of blue light from quartz-tungsten-halogen (QTH), plasma-arc (PAC), or argon laser sources in monolayer culture. Post-light SDH activity correlated positively with population doubling time (R2 = 0.91 for PAC, 0.76 for QTH, 0.68 for laser); SDH activity increased for cell types with the longest doubling times and was suppressed for cell types with shorter doubling times. Thus, light-induced exposure differentially affects SDH activity, cell survival, and growth, depending on cell energy consumption. Blue light may be useful as a therapeutic modulator of cell growth and survival.

Original languageEnglish (US)
Pages (from-to)104-108
Number of pages5
JournalJournal of Dental Research
Volume83
Issue number2
DOIs
StatePublished - Jan 1 2004

Fingerprint

Cell Survival
Succinate Dehydrogenase
Light
Growth
Tungsten
Quartz
Halogens
Lasers
Argon
Population

Keywords

  • Fibroblasts
  • Keratinocytes
  • MTT
  • Visible light
  • in vitro

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Blue light differentially modulates cell survival and growth. / Wataha, J. C.; Lewis, J. B.; Lockwood, P. E.; Hsu, Stephen; Messer, Regina L W; Rueggeberg, Frederick; Bouillaguet, S.

In: Journal of Dental Research, Vol. 83, No. 2, 01.01.2004, p. 104-108.

Research output: Contribution to journalArticle

Wataha, J. C. ; Lewis, J. B. ; Lockwood, P. E. ; Hsu, Stephen ; Messer, Regina L W ; Rueggeberg, Frederick ; Bouillaguet, S. / Blue light differentially modulates cell survival and growth. In: Journal of Dental Research. 2004 ; Vol. 83, No. 2. pp. 104-108.
@article{c549c6449e2b4011b2f6adbab9272618,
title = "Blue light differentially modulates cell survival and growth",
abstract = "Previous studies have reported that blue light (400-500 nm) inhibits cell mitochondrial activity. We investigated the hypothesis that cells with high energy consumption are most susceptible to blue-light-induced mitochondrial inhibition. We estimated cell energy consumption by population doubling time, and cell survival and growth by succinate dehydrogenase (SDH) activity. Six cell types were exposed to 5 or 60 J/cm2 of blue light from quartz-tungsten-halogen (QTH), plasma-arc (PAC), or argon laser sources in monolayer culture. Post-light SDH activity correlated positively with population doubling time (R2 = 0.91 for PAC, 0.76 for QTH, 0.68 for laser); SDH activity increased for cell types with the longest doubling times and was suppressed for cell types with shorter doubling times. Thus, light-induced exposure differentially affects SDH activity, cell survival, and growth, depending on cell energy consumption. Blue light may be useful as a therapeutic modulator of cell growth and survival.",
keywords = "Fibroblasts, Keratinocytes, MTT, Visible light, in vitro",
author = "Wataha, {J. C.} and Lewis, {J. B.} and Lockwood, {P. E.} and Stephen Hsu and Messer, {Regina L W} and Frederick Rueggeberg and S. Bouillaguet",
year = "2004",
month = "1",
day = "1",
doi = "10.1177/154405910408300204",
language = "English (US)",
volume = "83",
pages = "104--108",
journal = "Journal of Dental Research",
issn = "0022-0345",
publisher = "SAGE Publications Inc.",
number = "2",

}

TY - JOUR

T1 - Blue light differentially modulates cell survival and growth

AU - Wataha, J. C.

AU - Lewis, J. B.

AU - Lockwood, P. E.

AU - Hsu, Stephen

AU - Messer, Regina L W

AU - Rueggeberg, Frederick

AU - Bouillaguet, S.

PY - 2004/1/1

Y1 - 2004/1/1

N2 - Previous studies have reported that blue light (400-500 nm) inhibits cell mitochondrial activity. We investigated the hypothesis that cells with high energy consumption are most susceptible to blue-light-induced mitochondrial inhibition. We estimated cell energy consumption by population doubling time, and cell survival and growth by succinate dehydrogenase (SDH) activity. Six cell types were exposed to 5 or 60 J/cm2 of blue light from quartz-tungsten-halogen (QTH), plasma-arc (PAC), or argon laser sources in monolayer culture. Post-light SDH activity correlated positively with population doubling time (R2 = 0.91 for PAC, 0.76 for QTH, 0.68 for laser); SDH activity increased for cell types with the longest doubling times and was suppressed for cell types with shorter doubling times. Thus, light-induced exposure differentially affects SDH activity, cell survival, and growth, depending on cell energy consumption. Blue light may be useful as a therapeutic modulator of cell growth and survival.

AB - Previous studies have reported that blue light (400-500 nm) inhibits cell mitochondrial activity. We investigated the hypothesis that cells with high energy consumption are most susceptible to blue-light-induced mitochondrial inhibition. We estimated cell energy consumption by population doubling time, and cell survival and growth by succinate dehydrogenase (SDH) activity. Six cell types were exposed to 5 or 60 J/cm2 of blue light from quartz-tungsten-halogen (QTH), plasma-arc (PAC), or argon laser sources in monolayer culture. Post-light SDH activity correlated positively with population doubling time (R2 = 0.91 for PAC, 0.76 for QTH, 0.68 for laser); SDH activity increased for cell types with the longest doubling times and was suppressed for cell types with shorter doubling times. Thus, light-induced exposure differentially affects SDH activity, cell survival, and growth, depending on cell energy consumption. Blue light may be useful as a therapeutic modulator of cell growth and survival.

KW - Fibroblasts

KW - Keratinocytes

KW - MTT

KW - Visible light

KW - in vitro

UR - http://www.scopus.com/inward/record.url?scp=4143141887&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4143141887&partnerID=8YFLogxK

U2 - 10.1177/154405910408300204

DO - 10.1177/154405910408300204

M3 - Article

C2 - 14742645

AN - SCOPUS:4143141887

VL - 83

SP - 104

EP - 108

JO - Journal of Dental Research

JF - Journal of Dental Research

SN - 0022-0345

IS - 2

ER -