Abstract
Previous studies have reported that blue light (400-500 nm) inhibits cell mitochondrial activity. We investigated the hypothesis that cells with high energy consumption are most susceptible to blue-light-induced mitochondrial inhibition. We estimated cell energy consumption by population doubling time, and cell survival and growth by succinate dehydrogenase (SDH) activity. Six cell types were exposed to 5 or 60 J/cm2 of blue light from quartz-tungsten-halogen (QTH), plasma-arc (PAC), or argon laser sources in monolayer culture. Post-light SDH activity correlated positively with population doubling time (R2 = 0.91 for PAC, 0.76 for QTH, 0.68 for laser); SDH activity increased for cell types with the longest doubling times and was suppressed for cell types with shorter doubling times. Thus, light-induced exposure differentially affects SDH activity, cell survival, and growth, depending on cell energy consumption. Blue light may be useful as a therapeutic modulator of cell growth and survival.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 104-108 |
| Number of pages | 5 |
| Journal | Journal of Dental Research |
| Volume | 83 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 2004 |
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Keywords
- Fibroblasts
- Keratinocytes
- MTT
- Visible light
- in vitro
ASJC Scopus subject areas
- Dentistry(all)
Cite this
Blue light differentially modulates cell survival and growth. / Wataha, J. C.; Lewis, J. B.; Lockwood, P. E.; Hsu, Stephen; Messer, Regina L W; Rueggeberg, Frederick; Bouillaguet, S.
In: Journal of Dental Research, Vol. 83, No. 2, 01.01.2004, p. 104-108.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Blue light differentially modulates cell survival and growth
AU - Wataha, J. C.
AU - Lewis, J. B.
AU - Lockwood, P. E.
AU - Hsu, Stephen
AU - Messer, Regina L W
AU - Rueggeberg, Frederick
AU - Bouillaguet, S.
PY - 2004/1/1
Y1 - 2004/1/1
N2 - Previous studies have reported that blue light (400-500 nm) inhibits cell mitochondrial activity. We investigated the hypothesis that cells with high energy consumption are most susceptible to blue-light-induced mitochondrial inhibition. We estimated cell energy consumption by population doubling time, and cell survival and growth by succinate dehydrogenase (SDH) activity. Six cell types were exposed to 5 or 60 J/cm2 of blue light from quartz-tungsten-halogen (QTH), plasma-arc (PAC), or argon laser sources in monolayer culture. Post-light SDH activity correlated positively with population doubling time (R2 = 0.91 for PAC, 0.76 for QTH, 0.68 for laser); SDH activity increased for cell types with the longest doubling times and was suppressed for cell types with shorter doubling times. Thus, light-induced exposure differentially affects SDH activity, cell survival, and growth, depending on cell energy consumption. Blue light may be useful as a therapeutic modulator of cell growth and survival.
AB - Previous studies have reported that blue light (400-500 nm) inhibits cell mitochondrial activity. We investigated the hypothesis that cells with high energy consumption are most susceptible to blue-light-induced mitochondrial inhibition. We estimated cell energy consumption by population doubling time, and cell survival and growth by succinate dehydrogenase (SDH) activity. Six cell types were exposed to 5 or 60 J/cm2 of blue light from quartz-tungsten-halogen (QTH), plasma-arc (PAC), or argon laser sources in monolayer culture. Post-light SDH activity correlated positively with population doubling time (R2 = 0.91 for PAC, 0.76 for QTH, 0.68 for laser); SDH activity increased for cell types with the longest doubling times and was suppressed for cell types with shorter doubling times. Thus, light-induced exposure differentially affects SDH activity, cell survival, and growth, depending on cell energy consumption. Blue light may be useful as a therapeutic modulator of cell growth and survival.
KW - Fibroblasts
KW - Keratinocytes
KW - MTT
KW - Visible light
KW - in vitro
UR - http://www.scopus.com/inward/record.url?scp=4143141887&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4143141887&partnerID=8YFLogxK
U2 - 10.1177/154405910408300204
DO - 10.1177/154405910408300204
M3 - Article
C2 - 14742645
AN - SCOPUS:4143141887
VL - 83
SP - 104
EP - 108
JO - Journal of Dental Research
JF - Journal of Dental Research
SN - 0022-0345
IS - 2
ER -