Bonding performance of experimental bioactive/biomimetic self-etch adhesives doped with calcium-phosphate fillers and biomimetic analogs of phosphoproteins

Gabriel Abuna, Victor P. Feitosa, Americo Bortolazzo Correr, Giuseppe Cama, Marcelo Giannini, Mario A. Sinhoreti, David Henry Pashley, Salvatore Sauro

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objectives This study examined the bonding performance and dentin remineralization potential of an experimental adhesive containing calcium-phosphate (Ca/P) micro-fillers, and self-etching primers doped with phosphoprotein biomimetic analogs (polyacrylic acid-(PAA) and/or sodium trimetaphosphate-(TMP)). Methods Experimental self-etching primers doped with biomimetic analogs (PAA and/or TMP), and an adhesive containing Ca2+, PO4 −3-releasing micro-fillers (Ca/P) were formulated. Sound human dentin specimens were bonded and cut into sticks after aging (24 h or 6 months) under simulated pulpal pressure (20 cm H2O), and tested for microtensile bond strength (μTBS). Results were analyzed using two-way ANOVA and Tukey's test (p < 0.05). Interfacial silver nanoleakage was assessed using SEM. Remineralization of EDTA-demineralized dentin was assessed through FTIR and TEM ultrastructural analysis. Results Application of the Ca/P-doped adhesive with or without dentin pre-treatments with the primer containing both biomimetic analogs (PAA and TMP) promoted stable μTBS over 6 months. Conversely, μTBS of the control primer and filler-free adhesive significantly decreased after 6 months. Nanoleakage decreased within the resin-dentin interfaces created using the Ca/P-doped adhesives. EDTA-demineralized dentin specimens treated the Ca/P-doped adhesive and the primer containing PAA and TMP showed phosphate uptake (FTIR analysis), as well as deposition of needle-like crystallites at intrafibrillar level (TEM analysis). Significance The use of Ca/P-doped self-etching adhesives applied in combination with analogs of phosphoproteins provides durable resin-dentin bonds. This approach may represent a suitable bonding strategy for remineralization of intrafibrillar dentin collagen within the resin-dentin interface.

Original languageEnglish (US)
Pages (from-to)79-86
Number of pages8
JournalJournal of Dentistry
Volume52
DOIs
StatePublished - Sep 1 2016

Fingerprint

Biomimetics
Phosphoproteins
Dentin
Adhesives
Thymidine Monophosphate
carbopol 940
Fourier Transform Infrared Spectroscopy
Edetic Acid
calcium phosphate
Silver
Needles
Analysis of Variance
Collagen
Phosphates
Pressure

Keywords

  • Adhesive
  • Biomimetic remineralization
  • Collagen
  • Dentin
  • Self-etching

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Bonding performance of experimental bioactive/biomimetic self-etch adhesives doped with calcium-phosphate fillers and biomimetic analogs of phosphoproteins. / Abuna, Gabriel; Feitosa, Victor P.; Correr, Americo Bortolazzo; Cama, Giuseppe; Giannini, Marcelo; Sinhoreti, Mario A.; Pashley, David Henry; Sauro, Salvatore.

In: Journal of Dentistry, Vol. 52, 01.09.2016, p. 79-86.

Research output: Contribution to journalArticle

Abuna, Gabriel ; Feitosa, Victor P. ; Correr, Americo Bortolazzo ; Cama, Giuseppe ; Giannini, Marcelo ; Sinhoreti, Mario A. ; Pashley, David Henry ; Sauro, Salvatore. / Bonding performance of experimental bioactive/biomimetic self-etch adhesives doped with calcium-phosphate fillers and biomimetic analogs of phosphoproteins. In: Journal of Dentistry. 2016 ; Vol. 52. pp. 79-86.
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abstract = "Objectives This study examined the bonding performance and dentin remineralization potential of an experimental adhesive containing calcium-phosphate (Ca/P) micro-fillers, and self-etching primers doped with phosphoprotein biomimetic analogs (polyacrylic acid-(PAA) and/or sodium trimetaphosphate-(TMP)). Methods Experimental self-etching primers doped with biomimetic analogs (PAA and/or TMP), and an adhesive containing Ca2+, PO4 −3-releasing micro-fillers (Ca/P) were formulated. Sound human dentin specimens were bonded and cut into sticks after aging (24 h or 6 months) under simulated pulpal pressure (20 cm H2O), and tested for microtensile bond strength (μTBS). Results were analyzed using two-way ANOVA and Tukey's test (p < 0.05). Interfacial silver nanoleakage was assessed using SEM. Remineralization of EDTA-demineralized dentin was assessed through FTIR and TEM ultrastructural analysis. Results Application of the Ca/P-doped adhesive with or without dentin pre-treatments with the primer containing both biomimetic analogs (PAA and TMP) promoted stable μTBS over 6 months. Conversely, μTBS of the control primer and filler-free adhesive significantly decreased after 6 months. Nanoleakage decreased within the resin-dentin interfaces created using the Ca/P-doped adhesives. EDTA-demineralized dentin specimens treated the Ca/P-doped adhesive and the primer containing PAA and TMP showed phosphate uptake (FTIR analysis), as well as deposition of needle-like crystallites at intrafibrillar level (TEM analysis). Significance The use of Ca/P-doped self-etching adhesives applied in combination with analogs of phosphoproteins provides durable resin-dentin bonds. This approach may represent a suitable bonding strategy for remineralization of intrafibrillar dentin collagen within the resin-dentin interface.",
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author = "Gabriel Abuna and Feitosa, {Victor P.} and Correr, {Americo Bortolazzo} and Giuseppe Cama and Marcelo Giannini and Sinhoreti, {Mario A.} and Pashley, {David Henry} and Salvatore Sauro",
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T1 - Bonding performance of experimental bioactive/biomimetic self-etch adhesives doped with calcium-phosphate fillers and biomimetic analogs of phosphoproteins

AU - Abuna, Gabriel

AU - Feitosa, Victor P.

AU - Correr, Americo Bortolazzo

AU - Cama, Giuseppe

AU - Giannini, Marcelo

AU - Sinhoreti, Mario A.

AU - Pashley, David Henry

AU - Sauro, Salvatore

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N2 - Objectives This study examined the bonding performance and dentin remineralization potential of an experimental adhesive containing calcium-phosphate (Ca/P) micro-fillers, and self-etching primers doped with phosphoprotein biomimetic analogs (polyacrylic acid-(PAA) and/or sodium trimetaphosphate-(TMP)). Methods Experimental self-etching primers doped with biomimetic analogs (PAA and/or TMP), and an adhesive containing Ca2+, PO4 −3-releasing micro-fillers (Ca/P) were formulated. Sound human dentin specimens were bonded and cut into sticks after aging (24 h or 6 months) under simulated pulpal pressure (20 cm H2O), and tested for microtensile bond strength (μTBS). Results were analyzed using two-way ANOVA and Tukey's test (p < 0.05). Interfacial silver nanoleakage was assessed using SEM. Remineralization of EDTA-demineralized dentin was assessed through FTIR and TEM ultrastructural analysis. Results Application of the Ca/P-doped adhesive with or without dentin pre-treatments with the primer containing both biomimetic analogs (PAA and TMP) promoted stable μTBS over 6 months. Conversely, μTBS of the control primer and filler-free adhesive significantly decreased after 6 months. Nanoleakage decreased within the resin-dentin interfaces created using the Ca/P-doped adhesives. EDTA-demineralized dentin specimens treated the Ca/P-doped adhesive and the primer containing PAA and TMP showed phosphate uptake (FTIR analysis), as well as deposition of needle-like crystallites at intrafibrillar level (TEM analysis). Significance The use of Ca/P-doped self-etching adhesives applied in combination with analogs of phosphoproteins provides durable resin-dentin bonds. This approach may represent a suitable bonding strategy for remineralization of intrafibrillar dentin collagen within the resin-dentin interface.

AB - Objectives This study examined the bonding performance and dentin remineralization potential of an experimental adhesive containing calcium-phosphate (Ca/P) micro-fillers, and self-etching primers doped with phosphoprotein biomimetic analogs (polyacrylic acid-(PAA) and/or sodium trimetaphosphate-(TMP)). Methods Experimental self-etching primers doped with biomimetic analogs (PAA and/or TMP), and an adhesive containing Ca2+, PO4 −3-releasing micro-fillers (Ca/P) were formulated. Sound human dentin specimens were bonded and cut into sticks after aging (24 h or 6 months) under simulated pulpal pressure (20 cm H2O), and tested for microtensile bond strength (μTBS). Results were analyzed using two-way ANOVA and Tukey's test (p < 0.05). Interfacial silver nanoleakage was assessed using SEM. Remineralization of EDTA-demineralized dentin was assessed through FTIR and TEM ultrastructural analysis. Results Application of the Ca/P-doped adhesive with or without dentin pre-treatments with the primer containing both biomimetic analogs (PAA and TMP) promoted stable μTBS over 6 months. Conversely, μTBS of the control primer and filler-free adhesive significantly decreased after 6 months. Nanoleakage decreased within the resin-dentin interfaces created using the Ca/P-doped adhesives. EDTA-demineralized dentin specimens treated the Ca/P-doped adhesive and the primer containing PAA and TMP showed phosphate uptake (FTIR analysis), as well as deposition of needle-like crystallites at intrafibrillar level (TEM analysis). Significance The use of Ca/P-doped self-etching adhesives applied in combination with analogs of phosphoproteins provides durable resin-dentin bonds. This approach may represent a suitable bonding strategy for remineralization of intrafibrillar dentin collagen within the resin-dentin interface.

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KW - Collagen

KW - Dentin

KW - Self-etching

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