Bone generation in the reconstruction of a critical size calvarial defect in an experimental model

Yong Chen Por, C. Raul Barceló, Kenneth E. Salyer, David G. Genecov, Karen Troxel, El Gendler, Mohammed Elsayed Elsalanty, Lynne A. Opperman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

This study was designed to investigate the optimal combination of known osteogenic biomaterials with shape conforming struts to achieve calvarial vault reconstruction, using a canine model. Eighteen adolescent beagles were divided equally into 6 groups. A critical-size defect of 6 x 2 cm traversed the sagittal suture. The biomaterials used for calvarial reconstruction were demineralized perforated bone matrix (DBM), recombinant human bone morphogenetic protein 2 (rhBMP2), and autogenous platelet-rich plasma (PRP). The struts used were cobalt chrome (metal) or resorbable plate. The groupings were as follows: 1) DBM + metal, 2) DBM + PRP + metal, 3) DBM + PRP + resorbable plate, 4) DBM + rhBMP2 + metal, 5) DBM + rhBMP2 + PRP + metal, and 6) DBM + rhBMP2 + resorbable plate. Animals were killed at 3 months after surgery. There was no mortality or major complications. Analysis was performed macroscopically and histologically and with computed tomography. There was complete bony regeneration in the rhBMP2 groups only. Non-rhBMP2 groups had minimal bony ingrowth from the defect edges and on the dural surface, a finding confirmed by computed tomographic scan and histology. Platelet-rich plasma did not enhance bone regeneration. Shape conformation was good with both metal and resorbable plate. rhBMP2, but not PRP, accelerated calvarial regeneration in 3 months. The DBMs in the rhBMP2 groups were substituted by new trabecular bone. Shape molding was good with both metal and resorbable plate.

Original languageEnglish (US)
Pages (from-to)383-392
Number of pages10
JournalJournal of Craniofacial Surgery
Volume19
Issue number2
DOIs
StatePublished - Mar 1 2008

Fingerprint

Bone Matrix
Platelet-Rich Plasma
Theoretical Models
Metals
Bone and Bones
Biocompatible Materials
Regeneration
Bone Regeneration
Cobalt
recombinant human bone morphogenetic protein-2
Sutures
Canidae
Histology
Tomography
Mortality

Keywords

  • Cranial vault reconstruction
  • Critical size calvarial defect
  • Metal struts
  • Resorbable plates
  • rhBMP2

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

Cite this

Por, Y. C., Barceló, C. R., Salyer, K. E., Genecov, D. G., Troxel, K., Gendler, E., ... Opperman, L. A. (2008). Bone generation in the reconstruction of a critical size calvarial defect in an experimental model. Journal of Craniofacial Surgery, 19(2), 383-392. https://doi.org/10.1097/SCS.0b013e318163e415

Bone generation in the reconstruction of a critical size calvarial defect in an experimental model. / Por, Yong Chen; Barceló, C. Raul; Salyer, Kenneth E.; Genecov, David G.; Troxel, Karen; Gendler, El; Elsalanty, Mohammed Elsayed; Opperman, Lynne A.

In: Journal of Craniofacial Surgery, Vol. 19, No. 2, 01.03.2008, p. 383-392.

Research output: Contribution to journalArticle

Por, YC, Barceló, CR, Salyer, KE, Genecov, DG, Troxel, K, Gendler, E, Elsalanty, ME & Opperman, LA 2008, 'Bone generation in the reconstruction of a critical size calvarial defect in an experimental model', Journal of Craniofacial Surgery, vol. 19, no. 2, pp. 383-392. https://doi.org/10.1097/SCS.0b013e318163e415
Por, Yong Chen ; Barceló, C. Raul ; Salyer, Kenneth E. ; Genecov, David G. ; Troxel, Karen ; Gendler, El ; Elsalanty, Mohammed Elsayed ; Opperman, Lynne A. / Bone generation in the reconstruction of a critical size calvarial defect in an experimental model. In: Journal of Craniofacial Surgery. 2008 ; Vol. 19, No. 2. pp. 383-392.
@article{aef17640825e494884ceb69ed97a5d45,
title = "Bone generation in the reconstruction of a critical size calvarial defect in an experimental model",
abstract = "This study was designed to investigate the optimal combination of known osteogenic biomaterials with shape conforming struts to achieve calvarial vault reconstruction, using a canine model. Eighteen adolescent beagles were divided equally into 6 groups. A critical-size defect of 6 x 2 cm traversed the sagittal suture. The biomaterials used for calvarial reconstruction were demineralized perforated bone matrix (DBM), recombinant human bone morphogenetic protein 2 (rhBMP2), and autogenous platelet-rich plasma (PRP). The struts used were cobalt chrome (metal) or resorbable plate. The groupings were as follows: 1) DBM + metal, 2) DBM + PRP + metal, 3) DBM + PRP + resorbable plate, 4) DBM + rhBMP2 + metal, 5) DBM + rhBMP2 + PRP + metal, and 6) DBM + rhBMP2 + resorbable plate. Animals were killed at 3 months after surgery. There was no mortality or major complications. Analysis was performed macroscopically and histologically and with computed tomography. There was complete bony regeneration in the rhBMP2 groups only. Non-rhBMP2 groups had minimal bony ingrowth from the defect edges and on the dural surface, a finding confirmed by computed tomographic scan and histology. Platelet-rich plasma did not enhance bone regeneration. Shape conformation was good with both metal and resorbable plate. rhBMP2, but not PRP, accelerated calvarial regeneration in 3 months. The DBMs in the rhBMP2 groups were substituted by new trabecular bone. Shape molding was good with both metal and resorbable plate.",
keywords = "Cranial vault reconstruction, Critical size calvarial defect, Metal struts, Resorbable plates, rhBMP2",
author = "Por, {Yong Chen} and Barcel{\'o}, {C. Raul} and Salyer, {Kenneth E.} and Genecov, {David G.} and Karen Troxel and El Gendler and Elsalanty, {Mohammed Elsayed} and Opperman, {Lynne A.}",
year = "2008",
month = "3",
day = "1",
doi = "10.1097/SCS.0b013e318163e415",
language = "English (US)",
volume = "19",
pages = "383--392",
journal = "Journal of Craniofacial Surgery",
issn = "1049-2275",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Bone generation in the reconstruction of a critical size calvarial defect in an experimental model

AU - Por, Yong Chen

AU - Barceló, C. Raul

AU - Salyer, Kenneth E.

AU - Genecov, David G.

AU - Troxel, Karen

AU - Gendler, El

AU - Elsalanty, Mohammed Elsayed

AU - Opperman, Lynne A.

PY - 2008/3/1

Y1 - 2008/3/1

N2 - This study was designed to investigate the optimal combination of known osteogenic biomaterials with shape conforming struts to achieve calvarial vault reconstruction, using a canine model. Eighteen adolescent beagles were divided equally into 6 groups. A critical-size defect of 6 x 2 cm traversed the sagittal suture. The biomaterials used for calvarial reconstruction were demineralized perforated bone matrix (DBM), recombinant human bone morphogenetic protein 2 (rhBMP2), and autogenous platelet-rich plasma (PRP). The struts used were cobalt chrome (metal) or resorbable plate. The groupings were as follows: 1) DBM + metal, 2) DBM + PRP + metal, 3) DBM + PRP + resorbable plate, 4) DBM + rhBMP2 + metal, 5) DBM + rhBMP2 + PRP + metal, and 6) DBM + rhBMP2 + resorbable plate. Animals were killed at 3 months after surgery. There was no mortality or major complications. Analysis was performed macroscopically and histologically and with computed tomography. There was complete bony regeneration in the rhBMP2 groups only. Non-rhBMP2 groups had minimal bony ingrowth from the defect edges and on the dural surface, a finding confirmed by computed tomographic scan and histology. Platelet-rich plasma did not enhance bone regeneration. Shape conformation was good with both metal and resorbable plate. rhBMP2, but not PRP, accelerated calvarial regeneration in 3 months. The DBMs in the rhBMP2 groups were substituted by new trabecular bone. Shape molding was good with both metal and resorbable plate.

AB - This study was designed to investigate the optimal combination of known osteogenic biomaterials with shape conforming struts to achieve calvarial vault reconstruction, using a canine model. Eighteen adolescent beagles were divided equally into 6 groups. A critical-size defect of 6 x 2 cm traversed the sagittal suture. The biomaterials used for calvarial reconstruction were demineralized perforated bone matrix (DBM), recombinant human bone morphogenetic protein 2 (rhBMP2), and autogenous platelet-rich plasma (PRP). The struts used were cobalt chrome (metal) or resorbable plate. The groupings were as follows: 1) DBM + metal, 2) DBM + PRP + metal, 3) DBM + PRP + resorbable plate, 4) DBM + rhBMP2 + metal, 5) DBM + rhBMP2 + PRP + metal, and 6) DBM + rhBMP2 + resorbable plate. Animals were killed at 3 months after surgery. There was no mortality or major complications. Analysis was performed macroscopically and histologically and with computed tomography. There was complete bony regeneration in the rhBMP2 groups only. Non-rhBMP2 groups had minimal bony ingrowth from the defect edges and on the dural surface, a finding confirmed by computed tomographic scan and histology. Platelet-rich plasma did not enhance bone regeneration. Shape conformation was good with both metal and resorbable plate. rhBMP2, but not PRP, accelerated calvarial regeneration in 3 months. The DBMs in the rhBMP2 groups were substituted by new trabecular bone. Shape molding was good with both metal and resorbable plate.

KW - Cranial vault reconstruction

KW - Critical size calvarial defect

KW - Metal struts

KW - Resorbable plates

KW - rhBMP2

UR - http://www.scopus.com/inward/record.url?scp=42049106205&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42049106205&partnerID=8YFLogxK

U2 - 10.1097/SCS.0b013e318163e415

DO - 10.1097/SCS.0b013e318163e415

M3 - Article

C2 - 18362715

AN - SCOPUS:42049106205

VL - 19

SP - 383

EP - 392

JO - Journal of Craniofacial Surgery

JF - Journal of Craniofacial Surgery

SN - 1049-2275

IS - 2

ER -