Bone marrow as a source of endothelial cells and NeuN-expressing cells after stroke

David C Hess, William D Hill, Angeline Martin-Studdard, James Edwin Carroll, Joanna Brailer, Jo Carothers

Research output: Contribution to journalArticle

240 Citations (Scopus)

Abstract

Background and Purpose - After an ischemic event, bone marrow-derived cells may be involved in reparative processes. There is increasing evidence that bone marrow-derived stem cells may be a source of endothelial cells and organ-specific cells. Our objectives were to determine whether bone marrow-derived cells were a source of endothelial cells and neurons after cerebral ischemia. Methods - We transplanted bone marrow from male C57 BL/6-TgN (ACTbEGFP)1Osb mice, which express green fluorescent protein (GFP), into female C57 BL/6J mice. The recipient mice then underwent suture occlusion of the middle cerebral artery (MCA), and bone marrow- derived cells were tracked by GFP epifluorescence and Y chromosome probe. Results - Within 3 days and at 7 and 14 days after MCA occlusion, bone marrow-derived cells incorporated into the vasculature in the ischemic zone and expressed an endothelial cell phenotype. Few bone marrow-derived cells incorporated into the vasculature 24 hours after MCA occlusion. Some bone marrow-derived cells also expressed the neuronal marker NeuN at 7 and 14 days after ischemia. Conclusions - Postnatal vasculogenesis occurs in the brain in the setting of a cerebral infarction. Bone marrow-derived cells are a source of endothelial cells and NeuN-expressing cells after cerebral infarction. This plasticity may be exploited in the future to enhance recovery after stroke.

Original languageEnglish (US)
Pages (from-to)1362-1368
Number of pages7
JournalStroke
Volume33
Issue number5
DOIs
StatePublished - May 27 2002

Fingerprint

Bone Marrow Cells
Endothelial Cells
Bone Marrow
Stroke
Middle Cerebral Artery Infarction
Cerebral Infarction
Green Fluorescent Proteins
Y Chromosome
Brain Ischemia
Sutures
Stem Cells
Ischemia
Phenotype
Neurons
Brain

Keywords

  • Bone marrow
  • Cerebral ischemia
  • Endothelial
  • Regeneration

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Neuroscience(all)

Cite this

Bone marrow as a source of endothelial cells and NeuN-expressing cells after stroke. / Hess, David C; Hill, William D; Martin-Studdard, Angeline; Carroll, James Edwin; Brailer, Joanna; Carothers, Jo.

In: Stroke, Vol. 33, No. 5, 27.05.2002, p. 1362-1368.

Research output: Contribution to journalArticle

Hess, David C ; Hill, William D ; Martin-Studdard, Angeline ; Carroll, James Edwin ; Brailer, Joanna ; Carothers, Jo. / Bone marrow as a source of endothelial cells and NeuN-expressing cells after stroke. In: Stroke. 2002 ; Vol. 33, No. 5. pp. 1362-1368.
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