Bone marrow grafts restore cerebral blood flow and blood brain barrier in stroke rats

Cesario V. Borlongan, Jeffrey G. Lind, Ora Dillon-Carter, Guolong Yu, Martin Hadman, Charles Cheng, James Carroll, David C. Hess

Research output: Contribution to journalArticle

144 Scopus citations

Abstract

We monitored alterations in cerebral blood flow (CBF) and blood-brain barrier (BBB) permeability following middle cerebral artery occlusion (MCAo) and intrastriatal transplantation of mouse bone marrow stromal cells (BMSCs) or saline infusion in adult Sprague-Dawley rats. Laser Doppler and Evans Blue assay revealed that BMSC grafts dose-dependently restored CBF and BBB to near normal levels at a much earlier period (Days 4-5 post-MCAo) in transplanted stroke animals compared to stroke animals that received saline infusion (Days 11-14 post-MCAo). Xenografted BMSCs survived in the absence of immunosuppression, and elevated levels of transforming growth factor-beta superfamily of neurotrophic factors were detected in transplanted stroke animals. These data suggest that early restoration of CBF and BBB following transplantation of BMSCs could mediate the reported functional outcomes in stroke animals.

Original languageEnglish (US)
Pages (from-to)108-116
Number of pages9
JournalBrain Research
Volume1010
Issue number1-2
DOIs
StatePublished - Jun 4 2004

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Keywords

  • Cerebral ischemia
  • Green fluorescent protein
  • Immunosuppression
  • Neural transplantation
  • Neuroprotection
  • Stem cell
  • Striatum

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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