Bone response to fluoride exposure is influenced by genetics

Cláudia A.N. Kobayashi, Aline L. Leite, Camila Peres-Buzalaf, Juliane G. Carvalho, Gary M. Whitford, Eric T. Everett, Walter L. Siqueira, Marília A.R. Buzalaf

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Genetic factors influence the effects of fluoride (F) on amelogenesis and bone homeostasis but the underlying molecular mechanisms remain undefined. A label-free proteomics approach was employed to identify and evaluate changes in bone protein expression in two mouse strains having different susceptibilities to develop dental fluorosis and to alter bone quality. In vivo bone formation and histomorphometry after F intake were also evaluated and related to the proteome. Resistant 129P3/J and susceptible A/J mice were assigned to three groups given low-F food and water containing 0, 10 or 50 ppmF for 8 weeks. Plasma was evaluated for alkaline phosphatase activity. Femurs, tibiae and lumbar vertebrae were evaluated using micro-CT analysis and mineral apposition rate (MAR) was measured in cortical bone. For quantitative proteomic analysis, bone proteins were extracted and analyzed using liquid chromatography-electrospray ionizationtandem mass spectrometry (LC-ESI-MS/MS), followed by label-free semiquantitative differential expression analysis. Alterations in several bone proteins were found among the F treatment groups within each mouse strain and between the strains for each F treatment group (ratio ≥1.5 or ≤0.5; p<0.05). Although F treatment had no significant effects on BMD or bone histomorphometry in either strain, MAR was higher in the 50 ppmF 129P3/J mice than in the 50 ppmF A/J mice treated with 50 ppmF showing that F increased bone formation in a strain-specific manner. Also, F exposure was associated with dose-specific and strain-specific alterations in expression of proteins involved in osteogenesis and osteoclastogenesis. In conclusion, our findings confirm a genetic influence in bone response to F exposure and point to several proteins that may act as targets for the differential F responses in this tissue.

Original languageEnglish (US)
Article numbere114343
JournalPloS one
Volume9
Issue number12
DOIs
StatePublished - Dec 11 2014

Fingerprint

fluorides
Fluorides
Bone
bones
Bone and Bones
Osteogenesis
bone formation
mice
Proteins
Proteomics
Minerals
proteomics
Amelogenesis
Dental Fluorosis
protein synthesis
Lumbar Vertebrae
Labels
minerals
fluorosis
Electrospray Ionization Mass Spectrometry

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Kobayashi, C. A. N., Leite, A. L., Peres-Buzalaf, C., Carvalho, J. G., Whitford, G. M., Everett, E. T., ... Buzalaf, M. A. R. (2014). Bone response to fluoride exposure is influenced by genetics. PloS one, 9(12), [e114343]. https://doi.org/10.1371/journal.pone.0114343

Bone response to fluoride exposure is influenced by genetics. / Kobayashi, Cláudia A.N.; Leite, Aline L.; Peres-Buzalaf, Camila; Carvalho, Juliane G.; Whitford, Gary M.; Everett, Eric T.; Siqueira, Walter L.; Buzalaf, Marília A.R.

In: PloS one, Vol. 9, No. 12, e114343, 11.12.2014.

Research output: Contribution to journalArticle

Kobayashi, CAN, Leite, AL, Peres-Buzalaf, C, Carvalho, JG, Whitford, GM, Everett, ET, Siqueira, WL & Buzalaf, MAR 2014, 'Bone response to fluoride exposure is influenced by genetics', PloS one, vol. 9, no. 12, e114343. https://doi.org/10.1371/journal.pone.0114343
Kobayashi CAN, Leite AL, Peres-Buzalaf C, Carvalho JG, Whitford GM, Everett ET et al. Bone response to fluoride exposure is influenced by genetics. PloS one. 2014 Dec 11;9(12). e114343. https://doi.org/10.1371/journal.pone.0114343
Kobayashi, Cláudia A.N. ; Leite, Aline L. ; Peres-Buzalaf, Camila ; Carvalho, Juliane G. ; Whitford, Gary M. ; Everett, Eric T. ; Siqueira, Walter L. ; Buzalaf, Marília A.R. / Bone response to fluoride exposure is influenced by genetics. In: PloS one. 2014 ; Vol. 9, No. 12.
@article{e13850db014e40f2a938805d3eae6e8b,
title = "Bone response to fluoride exposure is influenced by genetics",
abstract = "Genetic factors influence the effects of fluoride (F) on amelogenesis and bone homeostasis but the underlying molecular mechanisms remain undefined. A label-free proteomics approach was employed to identify and evaluate changes in bone protein expression in two mouse strains having different susceptibilities to develop dental fluorosis and to alter bone quality. In vivo bone formation and histomorphometry after F intake were also evaluated and related to the proteome. Resistant 129P3/J and susceptible A/J mice were assigned to three groups given low-F food and water containing 0, 10 or 50 ppmF for 8 weeks. Plasma was evaluated for alkaline phosphatase activity. Femurs, tibiae and lumbar vertebrae were evaluated using micro-CT analysis and mineral apposition rate (MAR) was measured in cortical bone. For quantitative proteomic analysis, bone proteins were extracted and analyzed using liquid chromatography-electrospray ionizationtandem mass spectrometry (LC-ESI-MS/MS), followed by label-free semiquantitative differential expression analysis. Alterations in several bone proteins were found among the F treatment groups within each mouse strain and between the strains for each F treatment group (ratio ≥1.5 or ≤0.5; p<0.05). Although F treatment had no significant effects on BMD or bone histomorphometry in either strain, MAR was higher in the 50 ppmF 129P3/J mice than in the 50 ppmF A/J mice treated with 50 ppmF showing that F increased bone formation in a strain-specific manner. Also, F exposure was associated with dose-specific and strain-specific alterations in expression of proteins involved in osteogenesis and osteoclastogenesis. In conclusion, our findings confirm a genetic influence in bone response to F exposure and point to several proteins that may act as targets for the differential F responses in this tissue.",
author = "Kobayashi, {Cl{\'a}udia A.N.} and Leite, {Aline L.} and Camila Peres-Buzalaf and Carvalho, {Juliane G.} and Whitford, {Gary M.} and Everett, {Eric T.} and Siqueira, {Walter L.} and Buzalaf, {Mar{\'i}lia A.R.}",
year = "2014",
month = "12",
day = "11",
doi = "10.1371/journal.pone.0114343",
language = "English (US)",
volume = "9",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",

}

TY - JOUR

T1 - Bone response to fluoride exposure is influenced by genetics

AU - Kobayashi, Cláudia A.N.

AU - Leite, Aline L.

AU - Peres-Buzalaf, Camila

AU - Carvalho, Juliane G.

AU - Whitford, Gary M.

AU - Everett, Eric T.

AU - Siqueira, Walter L.

AU - Buzalaf, Marília A.R.

PY - 2014/12/11

Y1 - 2014/12/11

N2 - Genetic factors influence the effects of fluoride (F) on amelogenesis and bone homeostasis but the underlying molecular mechanisms remain undefined. A label-free proteomics approach was employed to identify and evaluate changes in bone protein expression in two mouse strains having different susceptibilities to develop dental fluorosis and to alter bone quality. In vivo bone formation and histomorphometry after F intake were also evaluated and related to the proteome. Resistant 129P3/J and susceptible A/J mice were assigned to three groups given low-F food and water containing 0, 10 or 50 ppmF for 8 weeks. Plasma was evaluated for alkaline phosphatase activity. Femurs, tibiae and lumbar vertebrae were evaluated using micro-CT analysis and mineral apposition rate (MAR) was measured in cortical bone. For quantitative proteomic analysis, bone proteins were extracted and analyzed using liquid chromatography-electrospray ionizationtandem mass spectrometry (LC-ESI-MS/MS), followed by label-free semiquantitative differential expression analysis. Alterations in several bone proteins were found among the F treatment groups within each mouse strain and between the strains for each F treatment group (ratio ≥1.5 or ≤0.5; p<0.05). Although F treatment had no significant effects on BMD or bone histomorphometry in either strain, MAR was higher in the 50 ppmF 129P3/J mice than in the 50 ppmF A/J mice treated with 50 ppmF showing that F increased bone formation in a strain-specific manner. Also, F exposure was associated with dose-specific and strain-specific alterations in expression of proteins involved in osteogenesis and osteoclastogenesis. In conclusion, our findings confirm a genetic influence in bone response to F exposure and point to several proteins that may act as targets for the differential F responses in this tissue.

AB - Genetic factors influence the effects of fluoride (F) on amelogenesis and bone homeostasis but the underlying molecular mechanisms remain undefined. A label-free proteomics approach was employed to identify and evaluate changes in bone protein expression in two mouse strains having different susceptibilities to develop dental fluorosis and to alter bone quality. In vivo bone formation and histomorphometry after F intake were also evaluated and related to the proteome. Resistant 129P3/J and susceptible A/J mice were assigned to three groups given low-F food and water containing 0, 10 or 50 ppmF for 8 weeks. Plasma was evaluated for alkaline phosphatase activity. Femurs, tibiae and lumbar vertebrae were evaluated using micro-CT analysis and mineral apposition rate (MAR) was measured in cortical bone. For quantitative proteomic analysis, bone proteins were extracted and analyzed using liquid chromatography-electrospray ionizationtandem mass spectrometry (LC-ESI-MS/MS), followed by label-free semiquantitative differential expression analysis. Alterations in several bone proteins were found among the F treatment groups within each mouse strain and between the strains for each F treatment group (ratio ≥1.5 or ≤0.5; p<0.05). Although F treatment had no significant effects on BMD or bone histomorphometry in either strain, MAR was higher in the 50 ppmF 129P3/J mice than in the 50 ppmF A/J mice treated with 50 ppmF showing that F increased bone formation in a strain-specific manner. Also, F exposure was associated with dose-specific and strain-specific alterations in expression of proteins involved in osteogenesis and osteoclastogenesis. In conclusion, our findings confirm a genetic influence in bone response to F exposure and point to several proteins that may act as targets for the differential F responses in this tissue.

UR - http://www.scopus.com/inward/record.url?scp=84917690492&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84917690492&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0114343

DO - 10.1371/journal.pone.0114343

M3 - Article

C2 - 25501567

AN - SCOPUS:84917690492

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 12

M1 - e114343

ER -