Bone surface and whole bone as biomarkers for acute fluoride exposure

Marília Afonso Rabelo Buzalaf, Elide Escolástico Caroselli, Juliane Guimarães De Carvalho, Rodrigo Cardoso De Oliveira, Vanessa Eid Da Silva Cardoso, Gary M. Whitford

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This study compares fluoride concentrations ([F]) in surface and whole bone for up to 27 days following an acute oral dose of F. Four groups of rats received single oral F dose (50 mg/kg body weight), and the control group received deionized water (n = 10/group). Groups were euthanized at 1, 3, 9, or 27 days after F administration. Plasma and femurs were collected. F on the femur surface was removed from a circular area (4.52 mm2) by immersion in 0.5M HCl for 15 s. The solution was buffered with total ionic strength adjustment buffer and analyzed with an electrode. The subjacent bone was sectioned and ashed at 600°C. Ash and plasma were analyzed for F with the electrode following hexamethyldisiloxane-facilitated diffusion. Data were analyzed by Kruskall-Wallis and Dunn's test and by linear regression (p < 0.05). Peak plasma and bone surface [F] occurred on day 1 (0.26 ± 0.14 μg/mL and 1801 ± 888 μg/g, respectively). Bone surface [F] at 3, 9, and 27 were not statistically different from control. A significant increase in whole bone [F] was observed 3 days after F administration and the [F] remained relatively constant thereafter. The mean (± SD) surface/whole bone [F] ratios for the control and F groups were 2.45 ± 0.98, 3.92 ± 1.32, 1.61 ± 0.82, 1.73 ± 0.39, and 1.09 ± 0.28, respectively. Plasma and bone surface [F]s were positively correlated (r = 0.74). Thus, bone surface was found to be a suitable biomarker for acute, sublethal F exposure 1 day after F administration. Whole bone [F] were significantly increased at 3, 9, and 27 days after F administration.

Original languageEnglish (US)
Pages (from-to)810-813
Number of pages4
JournalJournal of Analytical Toxicology
Volume29
Issue number8
DOIs
StatePublished - Jan 1 2005

Fingerprint

Biomarkers
Fluorides
fluoride
biomarker
bone
Bone
Bone and Bones
Plasmas
plasma
Ashes
Femur
Electrodes
electrode
Facilitated Diffusion
exposure
Control Groups
Deionized water
Immersion
Ionic strength
Linear regression

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

Cite this

Buzalaf, M. A. R., Caroselli, E. E., De Carvalho, J. G., De Oliveira, R. C., Da Silva Cardoso, V. E., & Whitford, G. M. (2005). Bone surface and whole bone as biomarkers for acute fluoride exposure. Journal of Analytical Toxicology, 29(8), 810-813. https://doi.org/10.1093/jat/29.8.810

Bone surface and whole bone as biomarkers for acute fluoride exposure. / Buzalaf, Marília Afonso Rabelo; Caroselli, Elide Escolástico; De Carvalho, Juliane Guimarães; De Oliveira, Rodrigo Cardoso; Da Silva Cardoso, Vanessa Eid; Whitford, Gary M.

In: Journal of Analytical Toxicology, Vol. 29, No. 8, 01.01.2005, p. 810-813.

Research output: Contribution to journalArticle

Buzalaf, MAR, Caroselli, EE, De Carvalho, JG, De Oliveira, RC, Da Silva Cardoso, VE & Whitford, GM 2005, 'Bone surface and whole bone as biomarkers for acute fluoride exposure', Journal of Analytical Toxicology, vol. 29, no. 8, pp. 810-813. https://doi.org/10.1093/jat/29.8.810
Buzalaf MAR, Caroselli EE, De Carvalho JG, De Oliveira RC, Da Silva Cardoso VE, Whitford GM. Bone surface and whole bone as biomarkers for acute fluoride exposure. Journal of Analytical Toxicology. 2005 Jan 1;29(8):810-813. https://doi.org/10.1093/jat/29.8.810
Buzalaf, Marília Afonso Rabelo ; Caroselli, Elide Escolástico ; De Carvalho, Juliane Guimarães ; De Oliveira, Rodrigo Cardoso ; Da Silva Cardoso, Vanessa Eid ; Whitford, Gary M. / Bone surface and whole bone as biomarkers for acute fluoride exposure. In: Journal of Analytical Toxicology. 2005 ; Vol. 29, No. 8. pp. 810-813.
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abstract = "This study compares fluoride concentrations ([F]) in surface and whole bone for up to 27 days following an acute oral dose of F. Four groups of rats received single oral F dose (50 mg/kg body weight), and the control group received deionized water (n = 10/group). Groups were euthanized at 1, 3, 9, or 27 days after F administration. Plasma and femurs were collected. F on the femur surface was removed from a circular area (4.52 mm2) by immersion in 0.5M HCl for 15 s. The solution was buffered with total ionic strength adjustment buffer and analyzed with an electrode. The subjacent bone was sectioned and ashed at 600°C. Ash and plasma were analyzed for F with the electrode following hexamethyldisiloxane-facilitated diffusion. Data were analyzed by Kruskall-Wallis and Dunn's test and by linear regression (p < 0.05). Peak plasma and bone surface [F] occurred on day 1 (0.26 ± 0.14 μg/mL and 1801 ± 888 μg/g, respectively). Bone surface [F] at 3, 9, and 27 were not statistically different from control. A significant increase in whole bone [F] was observed 3 days after F administration and the [F] remained relatively constant thereafter. The mean (± SD) surface/whole bone [F] ratios for the control and F groups were 2.45 ± 0.98, 3.92 ± 1.32, 1.61 ± 0.82, 1.73 ± 0.39, and 1.09 ± 0.28, respectively. Plasma and bone surface [F]s were positively correlated (r = 0.74). Thus, bone surface was found to be a suitable biomarker for acute, sublethal F exposure 1 day after F administration. Whole bone [F] were significantly increased at 3, 9, and 27 days after F administration.",
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