Bosentan inhibits oxidative and nitrosative stress and rescues occlusive pulmonaryhypertension

Olga Rafikova, Ruslan Rafikov, Sanjiv Kumar, Shruti Sharma, Saurabh Aggarwal, Frank Schneider, Danny Jonigk, Stephen Matthew Black, Stevan P. Tofovic

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Pulmonary arterial hypertension (PH) is a fatal disease marked by excessive pulmonary vascular cell proliferation. Patients with idiopathic PH express endothelin-1 (ET-1) at high levels in their lungs. As the activation of both types of ET-1 receptor (ETA and ETB) leads to increased generation of superoxide and hydrogen peroxide, this may contribute to the severe oxidative stress found in PH patients. As a number of pathways may induce oxidative stress, the particular role of ET-1 remains unclear. The aim of this study was to determine whether inhibition of ET-1 signaling could reduce pulmonary oxidative stress and attenuate the progression of disease in rats with occlusive-angioproliferative PH induced by a single dose of SU5416 (200 mg/kg) and subsequent exposure to hypoxia for 21 days. Using this regimen, animals developed severe PH as evidenced by a progressive increase in right-ventricle (RV) peak systolic pressure (RVPSP), severe RV hypertrophy, and pulmonary endothelial and smooth muscle cell proliferation, resulting in plexiform vasculopathy. PH rats also had increased oxidative stress, correlating with endothelial nitric oxide synthase uncoupling and NADPH oxidase activation, leading to enhanced protein nitration and increases in markers of vascular remodeling. Treatment with the combined ET receptor antagonist bosentan (250 mg/kg/day; day 10 to 21) prevented further increase in RVPSP and RV hypertrophy, decreased ETA/ETB protein levels, reduced oxidative stress and protein nitration, and resulted in marked attenuation of pulmonary vascular cell proliferation. We conclude that inhibition of ET-1 signaling significantly attenuates the oxidative and nitrosative stress associated with PH and prevents its progression.

Original languageEnglish (US)
Pages (from-to)28-43
Number of pages16
JournalFree Radical Biology and Medicine
Volume56
DOIs
StatePublished - Mar 1 2013

Fingerprint

Oxidative stress
Pulmonary Hypertension
Endothelin-1
Oxidative Stress
Cell proliferation
Nitration
Lung
Heart Ventricles
Cell Proliferation
Rats
Hypertrophy
Chemical activation
Blood Vessels
Endothelin A Receptors
Nitric Oxide Synthase Type III
NADPH Oxidase
Blood Pressure
Heat-Shock Proteins
Superoxides
Hydrogen Peroxide

Keywords

  • Endothelin
  • Free radicals
  • NADPH oxidase
  • Pulmonary hypertension
  • Uncoupled eNOS

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Bosentan inhibits oxidative and nitrosative stress and rescues occlusive pulmonaryhypertension. / Rafikova, Olga; Rafikov, Ruslan; Kumar, Sanjiv; Sharma, Shruti; Aggarwal, Saurabh; Schneider, Frank; Jonigk, Danny; Black, Stephen Matthew; Tofovic, Stevan P.

In: Free Radical Biology and Medicine, Vol. 56, 01.03.2013, p. 28-43.

Research output: Contribution to journalArticle

Rafikova, Olga ; Rafikov, Ruslan ; Kumar, Sanjiv ; Sharma, Shruti ; Aggarwal, Saurabh ; Schneider, Frank ; Jonigk, Danny ; Black, Stephen Matthew ; Tofovic, Stevan P. / Bosentan inhibits oxidative and nitrosative stress and rescues occlusive pulmonaryhypertension. In: Free Radical Biology and Medicine. 2013 ; Vol. 56. pp. 28-43.
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