Bradykinin-stimulated protein tyrosine phosphorylation promotes endothelial nitric oxide synthase translocation to the cytoskeleton

Virginia J. Venema, Mario B. Marrero, Richard C. Venema

Research output: Contribution to journalArticle

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Abstract

Stimulation of bovine aortic endothelial cells (BAEC) with bradykinin produces cycles of tyrosine phosphorylation/dephosphorylation of a 90 kDa endothelial nitric oxide synthase (eNOS)-associated protein which we have termed ENAP-1 (for endothelial nitric oxide synthase-associated protein 1). ENAP-1 interacts specifically and tightly with eNOS in BAEC and is co-immunoprecipitated from cell lysates with anti-eNOS antibodies. In addition, anti-phosphotyrosine antibodies co-precipitate eNOS. Bradykinin-stimulated tyrosine phosphorylation of ENAP-1 is blocked by the tyrosine kinase inhibitor, tyrphostin. Dephosphorylation is blocked by the tyrosine phosphatase inhibitor, orthovanadate. Treatment of BAEC with bradykinin or the tyrosine phosphatase inhibitor, phenylarsine oxide promotes tyrosine phosphorylation of detergent-insoluble, cytoskeletal proteins accompanied by translocation of eNOS to the cytoskeletal subcellular compartment. Translocation is blocked by the tyrosine kinase inhibitor, geldanamycin and does not appear to alter enzyme catalytic activity. Tyrosine phosphorylation-dependent association of eNOS with the cytoskeleton may have a role in targeting NO production to specific subcellular locations.

Original languageEnglish (US)
Pages (from-to)703-710
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume226
Issue number3
DOIs
StatePublished - Sep 24 1996

Fingerprint

Phosphorylation
Nitric Oxide Synthase Type III
Bradykinin
Cytoskeleton
Tyrosine
Proteins
Endothelial cells
Endothelial Cells
Phosphoric Monoester Hydrolases
Protein-Tyrosine Kinases
Tyrphostins
Phosphotyrosine
Cytoskeletal Proteins
Vanadates
Antibodies
Protein Transport
Detergents
Precipitates
Anti-Idiotypic Antibodies
Catalyst activity

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Bradykinin-stimulated protein tyrosine phosphorylation promotes endothelial nitric oxide synthase translocation to the cytoskeleton. / Venema, Virginia J.; Marrero, Mario B.; Venema, Richard C.

In: Biochemical and Biophysical Research Communications, Vol. 226, No. 3, 24.09.1996, p. 703-710.

Research output: Contribution to journalArticle

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