Bradykinin stimulates the tyrosine phosphorylation and bradykinin B2 receptor association of phospholipase Cγ1 in vascular endothelial cells

Virginia J. Venema, Hong Ju, Jimin Sun, Douglas C. Eaton, Mario B Marrero, Richard C Venema

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Bradykinin (BK) B2 receptor signaling involves activation of phospholipase C (PLC). PLC activation by other receptors consists of either allosteric activation of PLCβ isoforms by G-proteins or tyrosine phosphorylation of PLCγ isoforms. Because the B2 receptor is a G-protein-coupled receptor, it has been assumed that the receptor signals through PLCβ. In the present study, however, we have found that BK stimulation of IP3 production and the Ca2+ signal in endothelial cells is dependent on tyrosine phosphorylation. Furthermore, stimulation of B2 receptors in these cells is accompanied by a transient tyrosine phosphorylation of PLCγ1. Phosphorylation is correlated with increased IP3 production and association of PLCγ1 with the C-terminal intracellular domain of the B2 receptor. The B2 receptor can thus physically associate with intracellular proteins other than G-proteins. Activation of PLCγ isoforms, rather than PLCβ isoforms, may, therefore, be primarily responsible for BK-stimulated IP3 generation in endothelial cells.

Original languageEnglish (US)
Pages (from-to)70-75
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume246
Issue number1
DOIs
StatePublished - May 8 1998

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Bradykinin B2 Receptors
Phosphorylation
Endothelial cells
Type C Phospholipases
Bradykinin
Endothelial Cells
Association reactions
Protein Isoforms
Chemical activation
Tyrosine
GTP-Binding Proteins
G-Protein-Coupled Receptors
phospholipase C1
Tyr-bradykinin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Bradykinin stimulates the tyrosine phosphorylation and bradykinin B2 receptor association of phospholipase Cγ1 in vascular endothelial cells. / Venema, Virginia J.; Ju, Hong; Sun, Jimin; Eaton, Douglas C.; Marrero, Mario B; Venema, Richard C.

In: Biochemical and Biophysical Research Communications, Vol. 246, No. 1, 08.05.1998, p. 70-75.

Research output: Contribution to journalArticle

Venema, Virginia J. ; Ju, Hong ; Sun, Jimin ; Eaton, Douglas C. ; Marrero, Mario B ; Venema, Richard C. / Bradykinin stimulates the tyrosine phosphorylation and bradykinin B2 receptor association of phospholipase Cγ1 in vascular endothelial cells. In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 246, No. 1. pp. 70-75.
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