Brain-derived neurotrophic factor and collagen tubulization enhance functional recovery after peripheral nerve transection and repair

David S. Utley, Sheryl L. Lewin, Elbert T. Cheng, A. Neil Verity, David Sierra, David J Terris

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Objective: To investigate the effect of brain-derived neurotrophic factor (BDNF) and collagen tubulization (CT) on the regeneration of transected peripheral nerves. Methods and Design: The left sciatic nerve of 40 Sprague- Dawley rats was transected then repaired using one of four techniques: epineurial coaptation, CT, CT with BDNF delivered by an osmotic pump to the repair site, or CT with BDNF covalently cross-linked to the collagen matrix (CT/linked-BDNF). Sciatic functional indices were measured preoperatively and then postoperatively at 10-day intervals for 90 days. Segments of the sciatic nerves proximal and distal to the repair site were harvested at 90 days for histologic and morphometric evaluation. Results: Animals repaired by CT/linked-BDNF (n=10) demonstrated the most favorable functional recovery of all groups, with statistically significant differences seen compared with animals repaired by CT (n=10, P=.05) and epineurial coaptation (n=9, P<.001). Animals repaired by CT with BDNF delivered by an osmotic pump (n=8) and CT also showed statistically superior functional recovery compared with those repaired by epineurial coaptation (P=.005 and P=.02, respectively). Nerves repaired by CT/linked-BDNF had the largest mean axon diameters proximal and distal to the repair site. Conclusions: Brain-derived neurotrophic factor and CT improve the rate and the degree to which recovery of sciatic function occurs after nerve transection and repair. Animals repaired by CT/BDNF- linked demonstrated the most favorable functional recovery of all groups. Animals whose repair technique included BDNF had the largest mean axon diameters of all groups.

Original languageEnglish (US)
Pages (from-to)407-413
Number of pages7
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume122
Issue number4
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Fingerprint

Brain-Derived Neurotrophic Factor
Peripheral Nerves
Collagen
Sciatic Nerve
Axons
Recovery of Function
Sprague Dawley Rats
Regeneration

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

Cite this

Brain-derived neurotrophic factor and collagen tubulization enhance functional recovery after peripheral nerve transection and repair. / Utley, David S.; Lewin, Sheryl L.; Cheng, Elbert T.; Verity, A. Neil; Sierra, David; Terris, David J.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 122, No. 4, 01.01.1996, p. 407-413.

Research output: Contribution to journalArticle

Utley, David S. ; Lewin, Sheryl L. ; Cheng, Elbert T. ; Verity, A. Neil ; Sierra, David ; Terris, David J. / Brain-derived neurotrophic factor and collagen tubulization enhance functional recovery after peripheral nerve transection and repair. In: Archives of Otolaryngology - Head and Neck Surgery. 1996 ; Vol. 122, No. 4. pp. 407-413.
@article{765a3564e6534d079d958a3fe02fc02f,
title = "Brain-derived neurotrophic factor and collagen tubulization enhance functional recovery after peripheral nerve transection and repair",
abstract = "Objective: To investigate the effect of brain-derived neurotrophic factor (BDNF) and collagen tubulization (CT) on the regeneration of transected peripheral nerves. Methods and Design: The left sciatic nerve of 40 Sprague- Dawley rats was transected then repaired using one of four techniques: epineurial coaptation, CT, CT with BDNF delivered by an osmotic pump to the repair site, or CT with BDNF covalently cross-linked to the collagen matrix (CT/linked-BDNF). Sciatic functional indices were measured preoperatively and then postoperatively at 10-day intervals for 90 days. Segments of the sciatic nerves proximal and distal to the repair site were harvested at 90 days for histologic and morphometric evaluation. Results: Animals repaired by CT/linked-BDNF (n=10) demonstrated the most favorable functional recovery of all groups, with statistically significant differences seen compared with animals repaired by CT (n=10, P=.05) and epineurial coaptation (n=9, P<.001). Animals repaired by CT with BDNF delivered by an osmotic pump (n=8) and CT also showed statistically superior functional recovery compared with those repaired by epineurial coaptation (P=.005 and P=.02, respectively). Nerves repaired by CT/linked-BDNF had the largest mean axon diameters proximal and distal to the repair site. Conclusions: Brain-derived neurotrophic factor and CT improve the rate and the degree to which recovery of sciatic function occurs after nerve transection and repair. Animals repaired by CT/BDNF- linked demonstrated the most favorable functional recovery of all groups. Animals whose repair technique included BDNF had the largest mean axon diameters of all groups.",
author = "Utley, {David S.} and Lewin, {Sheryl L.} and Cheng, {Elbert T.} and Verity, {A. Neil} and David Sierra and Terris, {David J}",
year = "1996",
month = "1",
day = "1",
doi = "10.1001/archotol.1996.01890160047009",
language = "English (US)",
volume = "122",
pages = "407--413",
journal = "JAMA Otolaryngology - Head and Neck Surgery",
issn = "2168-6181",
publisher = "American Medical Association",
number = "4",

}

TY - JOUR

T1 - Brain-derived neurotrophic factor and collagen tubulization enhance functional recovery after peripheral nerve transection and repair

AU - Utley, David S.

AU - Lewin, Sheryl L.

AU - Cheng, Elbert T.

AU - Verity, A. Neil

AU - Sierra, David

AU - Terris, David J

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Objective: To investigate the effect of brain-derived neurotrophic factor (BDNF) and collagen tubulization (CT) on the regeneration of transected peripheral nerves. Methods and Design: The left sciatic nerve of 40 Sprague- Dawley rats was transected then repaired using one of four techniques: epineurial coaptation, CT, CT with BDNF delivered by an osmotic pump to the repair site, or CT with BDNF covalently cross-linked to the collagen matrix (CT/linked-BDNF). Sciatic functional indices were measured preoperatively and then postoperatively at 10-day intervals for 90 days. Segments of the sciatic nerves proximal and distal to the repair site were harvested at 90 days for histologic and morphometric evaluation. Results: Animals repaired by CT/linked-BDNF (n=10) demonstrated the most favorable functional recovery of all groups, with statistically significant differences seen compared with animals repaired by CT (n=10, P=.05) and epineurial coaptation (n=9, P<.001). Animals repaired by CT with BDNF delivered by an osmotic pump (n=8) and CT also showed statistically superior functional recovery compared with those repaired by epineurial coaptation (P=.005 and P=.02, respectively). Nerves repaired by CT/linked-BDNF had the largest mean axon diameters proximal and distal to the repair site. Conclusions: Brain-derived neurotrophic factor and CT improve the rate and the degree to which recovery of sciatic function occurs after nerve transection and repair. Animals repaired by CT/BDNF- linked demonstrated the most favorable functional recovery of all groups. Animals whose repair technique included BDNF had the largest mean axon diameters of all groups.

AB - Objective: To investigate the effect of brain-derived neurotrophic factor (BDNF) and collagen tubulization (CT) on the regeneration of transected peripheral nerves. Methods and Design: The left sciatic nerve of 40 Sprague- Dawley rats was transected then repaired using one of four techniques: epineurial coaptation, CT, CT with BDNF delivered by an osmotic pump to the repair site, or CT with BDNF covalently cross-linked to the collagen matrix (CT/linked-BDNF). Sciatic functional indices were measured preoperatively and then postoperatively at 10-day intervals for 90 days. Segments of the sciatic nerves proximal and distal to the repair site were harvested at 90 days for histologic and morphometric evaluation. Results: Animals repaired by CT/linked-BDNF (n=10) demonstrated the most favorable functional recovery of all groups, with statistically significant differences seen compared with animals repaired by CT (n=10, P=.05) and epineurial coaptation (n=9, P<.001). Animals repaired by CT with BDNF delivered by an osmotic pump (n=8) and CT also showed statistically superior functional recovery compared with those repaired by epineurial coaptation (P=.005 and P=.02, respectively). Nerves repaired by CT/linked-BDNF had the largest mean axon diameters proximal and distal to the repair site. Conclusions: Brain-derived neurotrophic factor and CT improve the rate and the degree to which recovery of sciatic function occurs after nerve transection and repair. Animals repaired by CT/BDNF- linked demonstrated the most favorable functional recovery of all groups. Animals whose repair technique included BDNF had the largest mean axon diameters of all groups.

UR - http://www.scopus.com/inward/record.url?scp=0029960960&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029960960&partnerID=8YFLogxK

U2 - 10.1001/archotol.1996.01890160047009

DO - 10.1001/archotol.1996.01890160047009

M3 - Article

C2 - 8600926

AN - SCOPUS:0029960960

VL - 122

SP - 407

EP - 413

JO - JAMA Otolaryngology - Head and Neck Surgery

JF - JAMA Otolaryngology - Head and Neck Surgery

SN - 2168-6181

IS - 4

ER -