Brain nitric oxide synthase levels increase in response to antenatal ethanol exposure

Maria L. Dizon, Lou Ann Bronw, Stephen Matthew Black

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Aims: Our previous in vitro data have indicated that ethanol can increase nitric oxide synthase (NOS) expression. Thus, the Aims of this study were to determine whether ethanol produces the same effect in vivo. Methods: To accomplish this, we utilized the well-established prenatal ethanol (EtOH) exposure model in the guinea pig to examine the effect on brain NOS expression and activity. Results: Brain homogenates isolated from offspring of guinea pigs fed EtOH exhibited an increase in NOS protein expression and NOS activity compared to controls. Increased expression of neuronal NOS was observed only in soluble fractions of brain homogenates (P < 0.05 vs. control). Increased expression of a ∼60 kDa band was detected in the soluble fraction that was immunoreactive against an antiserum raised against inducible NOS. In addition, an immunoreactive band of the correct predicted molecular weight for iNOS was found in the particulate fraction although the expression was unchanged between control and EtOH-treated animals. Endothelial NOS protein expression could not be detected in either soluble or particulate fractions from control or EtOH-treated animals. Conclusions: These results suggest that EtOH may exert its toxic effects antenatally via a mechanism of altered nitric oxide availability from NOS.

Original languageEnglish (US)
Pages (from-to)101-105
Number of pages5
JournalAlcohol and Alcoholism
Volume39
Issue number2
DOIs
StatePublished - Mar 1 2004

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Nitric Oxide Synthase
Brain
Ethanol
Guinea Pigs
Animals
Nitric Oxide Synthase Type I
Nitric Oxide Synthase Type III
Poisons
Nitric Oxide Synthase Type II
Immune Sera
Nitric Oxide
Proteins
Molecular Weight
Molecular weight
Availability

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Medicine(all)
  • Toxicology
  • Psychiatry and Mental health

Cite this

Brain nitric oxide synthase levels increase in response to antenatal ethanol exposure. / Dizon, Maria L.; Bronw, Lou Ann; Black, Stephen Matthew.

In: Alcohol and Alcoholism, Vol. 39, No. 2, 01.03.2004, p. 101-105.

Research output: Contribution to journalArticle

Dizon, Maria L. ; Bronw, Lou Ann ; Black, Stephen Matthew. / Brain nitric oxide synthase levels increase in response to antenatal ethanol exposure. In: Alcohol and Alcoholism. 2004 ; Vol. 39, No. 2. pp. 101-105.
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