Breaking biological barriers with a toothbrush

K. Amano, K. Miyake, J. L. Borke, Paul L McNeil

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Toothbrushing exposes epithelia and other tissues of the oral cavity to mechanical stress. Here, we investigated whether brushing induces cell wounding - plasma membrane disruption - in epithelial and other cell types in the oral cavity. Brushing of the gingivae and tongues of rats resulted in a striking increase in the number of cells positive for a marker of disruption injury. These cells included those in all strata of the gingival epithelium, and in the skeletal muscle of the tongue, Additionally, we found that brushing resulted in an increase in c-fos expression by junctional epithelial and skeletal muscle cells. Epithelial barrier function, however, was not overtly affected by brushing, despite the observed individual injuries to cells. We concluded that brushing disrupts cell plasma membrane barriers in the oral cavity and activates gene expression events that may lead to local adaptive changes in tissue architecture beneficial to gingival health.

Original languageEnglish (US)
Pages (from-to)769-774
Number of pages6
JournalJournal of Dental Research
Volume86
Issue number8
DOIs
StatePublished - Aug 1 2007

Fingerprint

Mouth
Cell Membrane
Tongue
Skeletal Muscle
Epithelium
Toothbrushing
Mechanical Stress
Wounds and Injuries
Gingiva
Muscle Cells
Cell Count
Epithelial Cells
Gene Expression
Health

Keywords

  • C-fos
  • Cell injury
  • Oral epithelium
  • Plasma membrane disruption

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Amano, K., Miyake, K., Borke, J. L., & McNeil, P. L. (2007). Breaking biological barriers with a toothbrush. Journal of Dental Research, 86(8), 769-774. https://doi.org/10.1177/154405910708600816

Breaking biological barriers with a toothbrush. / Amano, K.; Miyake, K.; Borke, J. L.; McNeil, Paul L.

In: Journal of Dental Research, Vol. 86, No. 8, 01.08.2007, p. 769-774.

Research output: Contribution to journalArticle

Amano, K, Miyake, K, Borke, JL & McNeil, PL 2007, 'Breaking biological barriers with a toothbrush', Journal of Dental Research, vol. 86, no. 8, pp. 769-774. https://doi.org/10.1177/154405910708600816
Amano, K. ; Miyake, K. ; Borke, J. L. ; McNeil, Paul L. / Breaking biological barriers with a toothbrush. In: Journal of Dental Research. 2007 ; Vol. 86, No. 8. pp. 769-774.
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