Abstract
Background. Brief ischemic episodes that induce stunning of the myocardium may also induce stunning of the coronary endothelium. To test this hypothesis, we examined both in vivo and in vitro responses of canine coronary arteries exposed to brief ischemia. Methods and Results. Functional recovery of the endothelium was examined in vivo during reperfusion after 15 minutes of ischemia. Vasodilatory responses to acetylcholine were severely impaired during the first hour of reperfusion but gradually improved over a 90-minute period after ischemia. The vasoconstrictive response to U46619 was enhanced for the first 30 minutes of reperfusion and returned to normal within 60 minutes. In vitro vasomotor responses to potassium chloride, acetylcholine, bradykinin, and sodium nitroprusside were examined in isolated segments of canine coronary arteries preexposed in vivo to brief ischemia (10-30 minutes) and 20 minutes of reperfusion. The results showed enhanced contractile responses and blunted endothelium-dependent but not endothelium-independent vasodilatory responses of arterial rings subjected to 10 minutes of ischemia. Twenty and 30 minutes of ischemia completely impaired endothelium-dependent vasodilation. When reperfusion was extended to 120 minutes after 15 minutes of ischemia, vasodilatory responses to acetylcholine had recovered by almost 90%. Examination of endothelial integrity by transmission electron microscopy after 10-15 minutes of ischemia revealed no evidence of structural damage. Twenty and 30 minutes of ischemia induced cytoplasmic vacuolation, partial detachment of endothelium, and swelling of cytoplasmic organelles. Conclusions. These data support the hypothesis that brief ischemia-reperfusion induces stunning of endothelium in which endothelium-dependent vasodilatory function is impaired temporarily without morphological damage.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1473-1482 |
| Number of pages | 10 |
| Journal | Circulation |
| Volume | 85 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jan 1 1992 |
| Externally published | Yes |
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Keywords
- Endothelium-derived relaxing factor
- Myocardial ischemia
- Vascular smooth muscle
- Vasodilators
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Physiology (medical)
Cite this
Brief ischemia-reperfusion induces stunning of endothelium in canine coronary artery. / Kim, Young D.; Fomsgaard, Jonna S.; Heim, Kurt F.; Ramwell, Peter W.; Thomas, George; Kagan, Elliott; Moore, Stephen P.; Coughlin, Steven Scott; Kuwahara, Maki; Analouei, Ali; Myers, Adam K.
In: Circulation, Vol. 85, No. 4, 01.01.1992, p. 1473-1482.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Brief ischemia-reperfusion induces stunning of endothelium in canine coronary artery
AU - Kim, Young D.
AU - Fomsgaard, Jonna S.
AU - Heim, Kurt F.
AU - Ramwell, Peter W.
AU - Thomas, George
AU - Kagan, Elliott
AU - Moore, Stephen P.
AU - Coughlin, Steven Scott
AU - Kuwahara, Maki
AU - Analouei, Ali
AU - Myers, Adam K.
PY - 1992/1/1
Y1 - 1992/1/1
N2 - Background. Brief ischemic episodes that induce stunning of the myocardium may also induce stunning of the coronary endothelium. To test this hypothesis, we examined both in vivo and in vitro responses of canine coronary arteries exposed to brief ischemia. Methods and Results. Functional recovery of the endothelium was examined in vivo during reperfusion after 15 minutes of ischemia. Vasodilatory responses to acetylcholine were severely impaired during the first hour of reperfusion but gradually improved over a 90-minute period after ischemia. The vasoconstrictive response to U46619 was enhanced for the first 30 minutes of reperfusion and returned to normal within 60 minutes. In vitro vasomotor responses to potassium chloride, acetylcholine, bradykinin, and sodium nitroprusside were examined in isolated segments of canine coronary arteries preexposed in vivo to brief ischemia (10-30 minutes) and 20 minutes of reperfusion. The results showed enhanced contractile responses and blunted endothelium-dependent but not endothelium-independent vasodilatory responses of arterial rings subjected to 10 minutes of ischemia. Twenty and 30 minutes of ischemia completely impaired endothelium-dependent vasodilation. When reperfusion was extended to 120 minutes after 15 minutes of ischemia, vasodilatory responses to acetylcholine had recovered by almost 90%. Examination of endothelial integrity by transmission electron microscopy after 10-15 minutes of ischemia revealed no evidence of structural damage. Twenty and 30 minutes of ischemia induced cytoplasmic vacuolation, partial detachment of endothelium, and swelling of cytoplasmic organelles. Conclusions. These data support the hypothesis that brief ischemia-reperfusion induces stunning of endothelium in which endothelium-dependent vasodilatory function is impaired temporarily without morphological damage.
AB - Background. Brief ischemic episodes that induce stunning of the myocardium may also induce stunning of the coronary endothelium. To test this hypothesis, we examined both in vivo and in vitro responses of canine coronary arteries exposed to brief ischemia. Methods and Results. Functional recovery of the endothelium was examined in vivo during reperfusion after 15 minutes of ischemia. Vasodilatory responses to acetylcholine were severely impaired during the first hour of reperfusion but gradually improved over a 90-minute period after ischemia. The vasoconstrictive response to U46619 was enhanced for the first 30 minutes of reperfusion and returned to normal within 60 minutes. In vitro vasomotor responses to potassium chloride, acetylcholine, bradykinin, and sodium nitroprusside were examined in isolated segments of canine coronary arteries preexposed in vivo to brief ischemia (10-30 minutes) and 20 minutes of reperfusion. The results showed enhanced contractile responses and blunted endothelium-dependent but not endothelium-independent vasodilatory responses of arterial rings subjected to 10 minutes of ischemia. Twenty and 30 minutes of ischemia completely impaired endothelium-dependent vasodilation. When reperfusion was extended to 120 minutes after 15 minutes of ischemia, vasodilatory responses to acetylcholine had recovered by almost 90%. Examination of endothelial integrity by transmission electron microscopy after 10-15 minutes of ischemia revealed no evidence of structural damage. Twenty and 30 minutes of ischemia induced cytoplasmic vacuolation, partial detachment of endothelium, and swelling of cytoplasmic organelles. Conclusions. These data support the hypothesis that brief ischemia-reperfusion induces stunning of endothelium in which endothelium-dependent vasodilatory function is impaired temporarily without morphological damage.
KW - Endothelium-derived relaxing factor
KW - Myocardial ischemia
KW - Vascular smooth muscle
KW - Vasodilators
UR - http://www.scopus.com/inward/record.url?scp=0026608336&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026608336&partnerID=8YFLogxK
U2 - 10.1161/01.CIR.85.4.1473
DO - 10.1161/01.CIR.85.4.1473
M3 - Article
C2 - 1555288
AN - SCOPUS:0026608336
VL - 85
SP - 1473
EP - 1482
JO - Circulation
JF - Circulation
SN - 0009-7322
IS - 4
ER -